Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
A synthetic peptide as a part of human, rat, mouse autophagy-related protein 3 (ATG3, APG3, APG3L, APG3-like, hApg3, protein PC3-96) conjugated to immunogenic carrier protein has been used as the immunogen.
Species Reactivity:
Human, mouse and rat. Other species not yet tested.
Applications:
Uses:
IHC-Paraffin/Frozen, WB 1 : 200 to 1 : 5000. The optimal dilution should be determined by the end user.
Dilutions:
Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot 1:200 to 1:5000
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Lyophilized Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. When reconstituting, glycerol (1:1) may be added for an additional stability.
Preservative:
No Preservative
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy. ; FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. ; SUBCELLULAR LOCATION: Cytoplasm ; TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.