ATG3/APG3 Antibody Summary
| Immunogen |
A synthetic peptide as a part of human, rat, mouse autophagy-related protein 3 (ATG3, APG3, APG3L, APG3-like, hApg3, protein PC3-96) conjugated to immunogenic carrier protein has been used as the immunogen. |
| Specificity |
ATG3. |
| Clonality |
Polyclonal |
| Host |
Sheep |
| Gene |
ATG3 |
| Purity |
Unpurified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunohistochemistry-Paraffin 1:10-1:500
- Western Blot 1:200-1:5000
|
| Control |
|
Reactivity Notes
Human, mouse and rat. Other species not yet tested.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
Whole antisera |
| Preservative |
No Preservative |
| Concentration |
LYOPH |
| Purity |
Unpurified |
| Reconstitution Instructions |
Reconstitute with 0.1 ml sterilized water to desired concentration. |
Alternate Names for ATG3/APG3 Antibody
Background
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy. ; FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. ; SUBCELLULAR LOCATION: Cytoplasm ; TISSUE SPECIFICITY: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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FAQs for ATG3/APG3 Antibody (NB110-74780) (0)
Control Lysate(s)
Secondary Antibodies
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