The specificity of this antibody has been confirmed by WB and IHC against the antigen.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
A synthetic peptide (NGMYYPQRQNTNKFN) corresponding to the amino acids 482-496 from human Angiopoietin 2.
Species Reactivity:
Cross reacts with Human and Rat. Predicted cross-reactivity based on sequence homology: Mouse.
Applications:
Uses:
Western Blotting (WB). Human Angiopoietin 2 (isoform 1) has a predicted length of 496 residues and MW of 57 kDa
Dilutions:
Western Blot 1.0 ug/ml
Unit Size:
0.1 mg
Concentration:
Lyoph
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Lyophilized with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4 Reconstitute in 0.1 ml of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Preservative:
0.05mg Thimerosal, 0.05mg Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Angiopoietin 2 and angiopoietin 1 have an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. The coiled coil domains mediate ligand homo-oligomerization and the fibrinogen-like domains mediate ligand activity. Recombinant human angiopoietin 2 has a molecular mass of approximately 66 kDa in SDS-PAGE under reducing and non-reducing conditions. Human and mouse angiopoietin 2 share 85% sequence identity and are 60% identical to their Ang1 homologs. Angiopoietin 2 and angiopoietin 1 are closely related endothelial cell-specific ligands of the endothelial cellspecific receptor Tie2 expressed on endothelial cells and early hematopoietic cells. Ang1, Ang2, and their receptor Tie2 play critical roles in embryogenic angiogenesis, adult vasculogenesis, and endothelial cell proliferation. Angiopoietin2, released by endothelial cells of interior tumor vessels, promotes vessel regression and disassociation from surrounding tumor cells. Both Ang1 and Ang2 induce phosphorylation of expressed receptors on NIH3T3 fibroblasts stably expressing transfected Tie2. However, only Ang1 induces a chemotactic response and Tie2 phosphorylation on endothelial cells. Ang2 inhibits Ang1 induced endothelial cell responses mediated by Tie2. Angiopoietin 2 probably acts as a natural antagonist for Ang1 and Tie2 both in vivo and in vitro. In adult mice and humans, angiopoietin 2 is expressed at sites of vascular remodeling.
This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.