Angiopoietin-2 Antibody Summary
| Immunogen |
A synthetic peptide (NGMYYPQRQNTNKFN) corresponding to the amino acids 482-496 from human Angiopoietin 2. |
| Specificity |
The specificity of this antibody has been confirmed by WB and IHC against the antigen. |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
ANGPT2 |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western Blotting (WB). Human Angiopoietin 2 (isoform 1) has a predicted length of 496 residues and MW of 57 kDa |
Reactivity Notes
Cross reacts with Human and Rat. Predicted cross-reactivity based on sequence identity: Mouse.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
0.2 mg DiSodium Phosphate, 0.9 mg NaCl and 5 mg BSA |
| Preservative |
0.05mg Sodium Azide |
| Concentration |
LYOPH |
| Purity |
Immunogen affinity purified |
| Reconstitution Instructions |
Reconstitute with 0.1 ml sterilized distilled water to a final concentration of 1.0 mg/ml. Centrifuge to remove any insoluble material. |
Alternate Names for Angiopoietin-2 Antibody
Background
Angiopoietin 2 and angiopoietin 1 have an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. The coiled coil domains mediate ligand homo-oligomerization and the fibrinogen-like domains mediate ligand activity. Recombinant human angiopoietin 2 has a molecular mass of approximately 66 kDa in SDS-PAGE under reducing and non-reducing conditions. Human and mouse angiopoietin 2 share 85% sequence identity and are 60% identical to their Ang1 homologs. Angiopoietin 2 and angiopoietin 1 are closely related endothelial cell-specific ligands of the endothelial cellspecific receptor Tie2 expressed on endothelial cells and early hematopoietic cells. Ang1, Ang2, and their receptor Tie2 play critical roles in embryogenic angiogenesis, adult vasculogenesis, and endothelial cell proliferation. Angiopoietin2, released by endothelial cells of interior tumor vessels, promotes vessel regression and disassociation from surrounding tumor cells. Both Ang1 and Ang2 induce phosphorylation of expressed receptors on NIH3T3 fibroblasts stably expressing transfected Tie2. However, only Ang1 induces a chemotactic response and Tie2 phosphorylation on endothelial cells. Ang2 inhibits Ang1 induced endothelial cell responses mediated by Tie2. Angiopoietin 2 probably acts as a natural antagonist for Ang1 and Tie2 both in vivo and in vitro. In adult mice and humans, angiopoietin 2 is expressed at sites of vascular remodeling.
This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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