Reacts with the Z-lines of mammalian and avian skeletal and cardiac myofibrils, including human. Also reacts with alpha actinin from non-muscle cells grown in tissue culture. Does not cross react with other cellular components as determined by IF.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
ELISA (1:500 - 1:2000). Western blot (under reducing conditions against Actinin (5ug/lane), a major band was observed at 100 kD. Smaller bands where also visible, but this is likely to be due to the impurities present). Immunofluorescence (it has been reported that this antibody is suitable for use in Immunofluorescence although this has not been determined in-house). IF and WB are minimum dilutions.
Dilutions:
ELISA 1:500 - 1:2000,
immunofluorescence 1:10000,
Western Blot 1:20000,
Immunohistochemistry-Frozen
Unit Size:
1.0 ml
Concentration:
3.5 mg/ml
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.