alpha-Actinin 1 Antibody (EA-53)

Name: alpha-Actinin 1 Antibody (EA-53)
Brand: Novus Biologicals
SKU: NB600-581
Price: 299 USD
Availability: Discontinued

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Catalog Number

NB600-581
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alpha-Actinin 1 Antibody (EA-53) Summary

Immunogen	Purified rabbit skeletal alpha-actinin.
Localization	Cytoplasmic
Marker	Z-disk Marker
Specificity	This is specific for alpha-skeletal muscle actinin and alpha-cardiac actinin. It stains Z lines and dots in stress fibers of myotubes in skeletal and cardiac muscle but not in non-sarcomeric muscle elements.
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	ACTN1
Purity	Unpurified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	Immunohistochemistry 1:25-1:50 Immunohistochemistry-Paraffin 1:25-1:50
Application Notes	This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. Suggested incubation period is 60 minutes at room temperature. Depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.

Reactivity Notes

Cross-reacts with Cow, Cat, Chicken, Dog, Fish, Goat, Lizard, Mouse, Pig, Rabbit, Rat, Sheep, Snake and Xenopus laevis. Not yet tested in other species.

Packaging, Storage & Formulations

Storage	Store at 4C. Do not freeze.
Buffer	Ascites
Preservative	Sodium Azide
Purity	Unpurified

Alternate Names for alpha-Actinin 1 Antibody (EA-53)

actinin 1 smooth muscle
actinin, alpha 1
ACTN1
alpha Actinin 1
alphaActinin 1
alpha-Actinin 1
Alpha-actinin cytoskeletal isoform
alpha-actinin-1
BDPLT15
F-actin cross-linking protein
FLJ40884
FLJ54432
Non-muscle alpha-actinin-1

Background

Alpha Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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alpha-Actinin 1
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NB600-581

Species: Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gt, Rb, Sh, Xp

Applications: IHC

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Bioinformatics

Gene Symbol	ACTN1
Entrez	87(Human) 109711(Mouse) 29437(Rat)
Uniprot	P12814()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

alpha-Actinin 1 Antibody (EA-53)

Species Glossary

Images

Product Details

Order Details

alpha-Actinin 1 Antibody (EA-53) Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for alpha-Actinin 1 Antibody (EA-53)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for alpha-Actinin 1 Antibody (NB600-581) (0)

Reviews for alpha-Actinin 1 Antibody (NB600-581) (0)

Product General Protocols

FAQs for alpha-Actinin 1 Antibody (NB600-581) (0)

Secondary Antibodies

Isotype Controls

Additional alpha-Actinin 1 Products

Research Areas for alpha-Actinin 1 Antibody (NB600-581)

Blogs on alpha-Actinin 1

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics