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![curve: adiponectin monoclonal antibody[NB100-72984]-Serum titration curve for the assay Adn20-Adn23. Pooled normal human serum was serially diluted with phosphatebuffered saline with EGTA or w/o EGTA (10 mM K-phosphate, pH 7.4, 150 mM NaCl, 0.1% Tween-20, 10 mM EGTA).](http://images.novusbio.com/images/100-72984.jpg "curve: adiponectin monoclonal antibody[NB100-72984]-Serum titration curve for the assay Adn20-Adn23. Pooled normal human serum was serially diluted with phosphatebuffered saline with EGTA or w/o EGTA (10 mM K-phosphate, pH 7.4, 150 mM NaCl, 0.1% Tween-20, 10 mM EGTA).")
![WB: adiponectin antibody[NB100-72984]-Immunodetection of native adiponectin with anti-Adn MAbs in WB after SDS-electrophoresis in reducing conditions. Lane 1 Adn20 Lane 2 Adn23 Lane 3 Adn63 Lane 4 Adn214 Lane 5 Adn222 Lane 6 Adn243.](http://images.novusbio.com/images/100-72984_2.jpg "WB: adiponectin antibody[NB100-72984]-Immunodetection of native adiponectin with anti-Adn MAbs in WB after SDS-electrophoresis in reducing conditions. Lane 1 Adn20 Lane 2 Adn23 Lane 3 Adn63 Lane 4 Adn214 Lane 5 Adn222 Lane 6 Adn243.")
![WB: adiponectin monoclonal antibody[NB100-72984]-SDS-electrophoresis in reducing conditions and WBing of native purified adiponectin from human plasma.](http://images.novusbio.com/images/100-72984_3.jpg "WB: adiponectin monoclonal antibody[NB100-72984]-SDS-electrophoresis in reducing conditions and WBing of native purified adiponectin from human plasma.")
![curve: adiponectin monoclonal antibody[NB100-72984]-Immunoreactivity in protein fractions after size-exclusion chromatography, measured by assay Adn20-Adn23. 1 ml of normal human serum was applied onto column. Positions of oligomeric forms of adiponectin and molecular weight markers are depicted on the picture. Black line is optical density detected at 280 nm](http://images.novusbio.com/images/100-72984_4.jpg "curve: adiponectin monoclonal antibody[NB100-72984]-Immunoreactivity in protein fractions after size-exclusion chromatography, measured by assay Adn20-Adn23. 1 ml of normal human serum was applied onto column. Positions of oligomeric forms of adiponectin and molecular weight markers are depicted on the picture. Black line is optical density detected at 280 nm")
