Recombinant Human Activin RIA/ALK-2/Activin Receptor Type 1 Protein

Purity is greater than 95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.

Activin RIA (also known as ACVR1 or ALK-2) is an activin type I receptor. The activin type I receptors transducer signals for a variety of members of the transforming growth factor (TGF) beta superfamily of ligands. This family of cytokine and hormones include activin, anti-mullerian hormone (AMH), bone morphogenetic proteins (BMPs) and nodal. Despite the large amount of processes that these ligands regulate, they all operate through essentially the same pathway: A ligand binds to a type II receptor, which recruits and transphosphorylates a type I receptor. The type I receptor recruits a receptor regulated SMAD (RSMAD) which it phosphorylates. The R-SMAD then translocates to the nucleus where it functions as a transcription factor. Activin RIA is expressed in normal parenchymal cells, endothelial cells, fibrobasts and tumor-derived epithelial cells. Defects in the receptor are a cause of fibrodysplasia ossificans progressive (FOP). This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Other Common Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible. Purified Activin RIA - FcB Chimera consists of 3-20% carbohydrate by weight.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Array NBP1-46037

• Activin RIA/ALK-2/Activin Receptor Type 1 Antibodies
• Activin RIA/ALK-2/Activin Receptor Type 1 ELISA Kits
• Activin RIA/ALK-2/Activin Receptor Type 1 Lysates
• Activin RIA/ALK-2/Activin Receptor Type 1 Proteins
• Activin RIA/ALK-2/Activin Receptor Type 1 Proteins and Enzymes