Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Human and mouse. Other species have not been tested.
Applications:
Uses:
The antibodies included in this pack are useful for Western blot and immunoprecipitation. See individual datasheets for suggested working dilutions.
Dilutions:
Immunoprecipitation, Western Blot
Unit Size:
2 vials
Concentration:
Concentration of individual antibodies may be found on the vial label.
Notes:
1 vial each of: NB600-532 and NB600-533.
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
See individual datasheets
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.
Western Blot: Actin antibody [NB600-533] - Detection of actin in 3T3 (20 ug) lysates using NB 600-533. ECL detection 30 seconds. Lane 1 - 1:5,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution
Western Blot: Actin antibody [NB600-533] - Detection of actin in 3T3 (20ug) lysates using NB 600-532. ECL detection 30 seconds. Lane 1 - 1:15,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution