Actin Alpha 2 Smooth Muscle Antibody (NB300-978)

Actin Alpha 2 Smooth Muscle Antibody

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Actin Alpha 2 Smooth Muscle Antibody Summary:
Species:Hu, Mu, Rt
Applications:IHC-P, PEP-ELISA, WB
Clonality:Polyclonal
Gene:ACTA2
Purity:Immunogen affinity purified
Host:Goat
Specificity:Variants NP_001604.1 and NP_001135417.1 encode the same protein.
Publications:Antibody has been mentioned in at least 1 Publication.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Actin Alpha 2 Smooth Muscle Antibody Details:
Isotype:IgG
Immunogen:Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1; NP_001135417.1.
Species Reactivity:

Human, Mouse, Rat, Dog

Applications:
Uses:This antibody is useful for Peptide ELISA, Immunohistochemistry-Paraffin and Western Blot. In WB an approx 45kDa band observed in Human Duodenum lysates (calculated MW of 42.0kDa according to NP_001604.1).
Dilutions:Immunohistochemistry-Paraffin, Peptide ELISA detection limit 1:1000, Western Blot 0.01-0.03 ug/ml
TMW:42 kDa
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:Tris-saline containing [pH7.3] 0.5% BSA
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ACTA2
Entrez59 (Human)
Swiss ProtP62736 (Human)
 
Background:

Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

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Actin Alpha 2 Smooth Muscle Antibody Images (1)
Western Blot: Smooth muscle alpha-actin Antibody [NB300-97]- staining (0.1ug/ml) of Human Duodenum lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour.
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Publications (1)

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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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RNAi (1)
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Secondary Antibodies
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Actin Alpha 2 Smooth Muscle in Context


Novus Explorer for Actin Alpha 2 Smooth Muscle gene

Try our bioinformatic tool that shows the relationships between ACTA2 and other genes, diseases, pathways and post-translational modifications.