Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1; NP_001135417.1.
Species Reactivity:
Human, Mouse, Rat, Dog
Applications:
Uses:
This antibody is useful for Peptide ELISA, Immunohistochemistry-Paraffin and Western Blot. In WB an approx 45kDa band observed in Human Duodenum lysates (calculated MW of 42.0kDa according to NP_001604.1).
Dilutions:
Immunohistochemistry-Paraffin, Peptide ELISA detection limit 1:1000, Western Blot 0.01-0.03 ug/ml
Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:
Tris-saline containing [pH7.3] 0.5% BSA
Preservative:
0.02% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Actin alpha 2, the human aortic smooth muscle actin gene, is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
Western Blot: Smooth muscle alpha-actin Antibody [NB300-97]- staining (0.1ug/ml) of Human Duodenum lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour.