Home » actin, gamma 2 » actin, gamma 2 Antibodies » actin, gamma 2 Antibody (HHF35)

We're moving to rndsystems.com. Come with us!

After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.

Register on R&D Systems

actin, gamma 2 Antibody (HHF35)

 Submit a Review

Images

 

Product Details

Summary
Product Discontinued
View other related actin, gamma 2 Primary Antibodies

Order Details


    • Catalog Number
      NB100-65713
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

actin, gamma 2 Antibody (HHF35) Summary

Immunogen
HHF35 is a mouse monoclonal IgG1 antibody derived by fusion of NS-1 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with an SDS-extracted protein fraction from human myocardium.
Specificity
HHF35 reacts with both alpha-muscle and gamma smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTG2
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:100
  • Western Blot 1:100-1:500
Application Notes
HHF35 is useful for immunohistochemistry on frozen and paraffin-embedded tissues preserved in several types of fixatives Westernblot, immuno-electron microscopy and ELISA. For immunohistochemical applications to paraffin embedded tissues it is recommended to dilute the antibody in PBS containing 50 mM EDTA..

Reactivity Notes

Cross reacts with Human, Monkey, Swine, Rabbit, Rat, Chicken and Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for actin, gamma 2 Antibody (HHF35)

  • ACT
  • ACTA3alpha-actin 3
  • ACTE
  • actin, gamma 2, smooth muscle, enteric
  • actin, gamma-enteric smooth muscle
  • ACTL3
  • ACTSGactin-like protein
  • Alpha-actin-3
  • Gamma-2-actin
  • smooth muscle gamma actin
  • Smooth muscle gamma-actin

Background

Among the six actin isoforms described in mammals, two are found in virtually all cells (beta and gamma cytoplasmic), two are detected in smooth muscle cells (alpha and beta-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. Alpha- muscle actin is present in striated as well as smooth muscle cells, and in pathological tissues derived therefrom. It has for example been detected in several types of muscle derived tumors, and also been shown to appear in stress fibers of fibroblastic cells involved in contractile phenomena such as wound healing and fibrocontractive diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Kallikrein 3/PSA
[HRP]
DKK300
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
(18 Publications)
Species: Hu
Applications: ELISA
Carboxypeptidase
B1/CPB1 Antibody - BS ...
NBP2-15698
Western Blot Carboxypeptidase B1/CPB1 Antibody - BSA Free
Western Blot Carboxypeptidase B1/CPB1 Antibody - BSA Free
Western Blot Carboxypeptidase B1/CPB1 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: IHC,  IHC-P, WB
Prothrombin
Antibody - BSA Free
NBP1-58268
Immunohistochemistry-Paraffin Prothrombin Antibody - BSA Free
Immunohistochemistry-Paraffin Prothrombin Antibody - BSA Free
Western Blot Prothrombin Antibody - BSA Free
(1 Review)
(3 Publications)
Species: Hu, Mu
Applications: IHC,  IHC-P, WB
C-Reactive
Protein/CRP [Biotin]
DY1707
N/A C-Reactive Protein/CRP [Biotin]
(75 Publications)
Species: Hu
Applications: ELISA
Serpin A3/alpha
1-Antichymotrypsin An ...
NBP2-52559
Western Blot Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) - BSA Free
ELISA Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) - BSA Free
Flow Cytometry Serpin A3/alpha 1-Antichymotrypsin Antibody (5G3C11) - BSA Free
(1 Publication)
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC,  IHC-P, WB
Apolipoprotein
E/ApoE Antibody (WUE- ...
NB110-60531
Flow (Intracellular) Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Western Blot Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Flow Cytometry Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
(2 Reviews)
(42 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC,  IHC-P, IP, WB
Serpin
C1/Antithrombin-III A ...
AF1267
Serpin C1/Antithrombin-III Antibody [Unconjugated]
(8 Publications)
Species: Hu
Applications: IP, Neut, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(716 Publications)
Species: Mu
Applications: ELISA
PSAT1 Antibody
NBP1-32920
Immunocytochemistry/ Immunofluorescence PSAT1 Antibody
Immunohistochemistry-Paraffin PSAT1 Antibody
Western Blot PSAT1 Antibody
(13 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC,  IHC-P, KD, WB
Protein S/PROS1
Antibody [Unconjugate
AF4036
Western Blot Protein S/PROS1 Antibody [Unconjugated]
Simple Western Protein S/PROS1 Antibody [Unconjugated]
(7 Publications)
Species: Hu
Applications: Simple Western, WB
PLAG1 Antibody
(3B7) - Azide and BSA ...
H00005324-M02
Immunohistochemistry PLAG1 Antibody (3B7) - Azide and BSA Free
Immunohistochemistry PLAG1 Antibody (3B7) - Azide and BSA Free
ELISA PLAG1 Antibody (3B7) - Azide and BSA Free
(20 Publications)
Species: Hu, Mu
Applications: ELISA, IHC,  IHC-P, WB

Puromycin-sensitive am ...
NBP3-48658
Western Blot Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)
Immunohistochemistry-Paraffin Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)
Immunocytochemistry/ Immunofluorescence Puromycin-sensitive aminopeptidase/NPEPPS Antibody (HL1531)
Species: Ca, Fe, Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC,  IHC-P, WB
beta Amyloid
Antibody (MOAB-2) - B ...
NBP2-13075
Immunohistochemistry-Frozen beta Amyloid Antibody (MOAB-2) - BSA Free
Immunohistochemistry beta Amyloid Antibody (MOAB-2) - BSA Free
Immunocytochemistry/ Immunofluorescence beta Amyloid Antibody (MOAB-2) - BSA Free
(2 Reviews)
(75 Publications)
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr,  IHC-P, IP, WB
CSRP1 Antibody
AF5739
Western Blot CSRP1 Antibody
Species: Hu, Mu, Rt
Applications: WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(850 Publications)
Species: Hu
Applications: BA
TFF2 Antibody
(366508) [Unconjugate
MAB4077
Immunohistochemistry TFF2 Antibody (366508) [Unconjugated]
(2 Publications)
Species: Hu
Applications: IHC, WB
Serpin A5/Protein
C Inhibitor Antibody ...
MAB1266
Intracellular Staining by Flow Cytometry Serpin A5/Protein C Inhibitor Antibody (210326) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, IP, ICFlow, WB
Serpin A3N
Antibody [Unconjugate
AF4709
Immunohistochemistry Serpin A3N Antibody [Unconjugated]
Western Blot Serpin A3N Antibody [Unconjugated]
(36 Publications)
Species: Mu
Applications: IP, WB
Presenilin-1
Antibody (APS 18)
NB100-74510
Western Blot Presenilin-1 Antibody (APS 18)
Immunocytochemistry/ Immunofluorescence Presenilin-1 Antibody (APS 18)
Immunocytochemistry/ Immunofluorescence Presenilin-1 Antibody (APS 18)
(1 Review)
(5 Publications)
Species: Hu, Mu, Pm, Rt
Applications: ELISA, ICC/IF, IHC,  IHC-P, IP, WB
actin, gamma 2
Antibody (HHF35)
NB100-65713
actin, gamma 2 Antibody (HHF35)
Species: Hu, Rt, Po, Ch, Pm, Rb, Ze
Applications: WB, ELISA, ICC/IF, IHC

Publications for actin, gamma 2 Antibody (NB100-65713) (0)

There are no publications for actin, gamma 2 Antibody (NB100-65713).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for actin, gamma 2 Antibody (NB100-65713) (0)

There are no reviews for actin, gamma 2 Antibody (NB100-65713). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for actin, gamma 2 Antibody (NB100-65713) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional actin, gamma 2 Products

Blogs on actin, gamma 2

There are no specific blogs for actin, gamma 2, but you can read our latest blog posts.

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our actin, gamma 2 Antibody (HHF35) and receive a gift card or discount.


Bioinformatics

Gene Symbol ACTG2
Entrez
Uniprot