This reacts with the alpha smooth muscle isoform of actin. It does not react with actin from fribroblasts, striated muscle and myocardium. This reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, and ovary.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The N-terminal decapeptide of alpha-smooth muscle actin.
Localization:
Cytoplasmic
Species Reactivity:
Cross-reacts with Human, Rabbit, Rat, Pig, Mouse and Baboon. Not yet tested in other species.
Applications:
Uses:
This antibody is useful for IHC, Western Blot, immunofluorescence and ELISA. Immunohistochemistry (formalin fixed paraffin embedded or frozen): 1/50 - 1/75 (30 minutes at RT). Immunocytochemistry: 1/75 Immunofluorescence: 1/25 -1/50 (cells) and 1/100 (vessel sections) This antibody can also be used on frozen tissue sections for IHC.
Dilutions:
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Unit Size:
0.5 ml
Concentration:
This product is unpurified. Concentration is not relevant.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.