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Actin Alpha 2 Smooth Muscle Antibody (NB120-18147)

Actin Alpha 2 Smooth Muscle Antibody (0.N.5)

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Actin Alpha 2 Smooth Muscle Antibody (0.N.5) Summary:
Species:Bb, Ch, Hu, Mu, Rb, Rt
Applications:IF, IHC-P
Clonality:Monoclonal
Gene:ACTA2
Purity:Protein A purified
Host:Mouse
Specificity:Recognizes the alpha-smooth muscle isoform of actin. Does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial).
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Actin Alpha 2 Smooth Muscle Antibody (0.N.5) Details:
Clone:0.N.5
Isotype:IgG2a Kappa
Immunogen:Synthetic peptide: MCEEEDSTAL, corresponding to N terminal amino acids 1-10 of alpha smooth muscle Actin.
Localization:Cytoplasmic
Epitope:Acetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth actin.
Species Reactivity:

Cross-reacts with Human, Mouse, Rat, Rabbit, Cow, Baboon and Chicken. Not yet tested in other species.

Applications:
Uses:This antibody is useful for Immunohistochemistry and Immunofluorescence.
Dilutions:immunofluorescence, Immunohistochemistry-Paraffin 1:400
Unit Size:0.25 ml
Concentration:0.02 mg/ml
Positive Controls:2 Positive Controls
Notes:
For IHC analysis, use at a dilution of 1:400 for 20 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:PBS; pH 7.4, 0.2% BSA, and 40% glycerol.
Preservative:0.09% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ACTA2
Entrez59 (Human)
109711 (Mouse)
81634 (Rat)
 
Background:

Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. This antibody reacts with tumors arising from smooth muscles and myoepithelial cells.
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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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RNAi (1)
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Actin Alpha 2 Smooth Muscle RNAiH00000059-R01Hu(1)(4)

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