Actin Alpha 1 Cardiac Muscle Antibody (EP184E) (NB110-55441)

Actin Alpha 1 Cardiac Muscle Antibody (EP184E) (NB110-55441) (0.1 ml)

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	Catalog Number	NB110-55441
	Size(s)	0.1 ml	Price:
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Actin Alpha 1 Cardiac Muscle Antibody (EP184E) Summary:

Species:	Hu, Mu, Rt	Gene:	ACTA1
Applications:	WB, FACS, ICC, IHC-P, IP	Purity:	Tissue culture supernatant
Clonality:	Monoclonal	Host:	Rabbit
Specificity:	This Actin antibody recognizes all muscle actins.
Publications:	NB110-55441 has at least 1 Background Reference

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Actin Alpha 1 Cardiac Muscle Antibody (EP184E) Details:

Clone:	EP184E
Isotype:	IgG
Immunogen:	A synthetic peptide corresponding to residues near the N-terminus of human Actin was used as an immunogen.

Species Reactivity:

Human, Mouse, Rat. For Mouse and Rat this antibody is recommended for Western Blot only.

Applications:

Uses:	This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 42kDa.
Dilutions:	Western Blot 1:1000-10000, Immunohistochemistry-Paraffin 1:100-200, Immunocytochemistry 1:250-500, Immunoprecipitation 1:20, Flow Cytometry 1:20
Unit Size:	0.1 ml
Concentration:	This product is unpurified. Concentration is not relevant.
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Buffer:	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol
Preservative:	0.01% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: ACTA1

Entrez:	58 (Human)
	11475 (Mouse)
	81633 (Rat)
	60 (ACTB)
	71 (ACTG1)
Uniprot:	P68032 (Human)

Related Genes:

Background:

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

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Actin Alpha 1 Cardiac Muscle Antibody (EP184E) Images (4)

	Western Blot: Actin (Pan) Antibody (EP184E) [NB110-55441] - Western blot analysis on A431 cell lysate using anti-Actin (NB110-55441), dilution 1:1,000

	Immunofluorescent: Actin (Pan) Antibody (EP184E) [NB110-55441] - Immunofluorescent staining of HeLa cells using anti-Actin

	Immunohistochemistry: Actin (Pan) Antibody (EP184E) [NB110-55441] - Immunohistochemical staining of paraffin-embedded human cardiac muscle using anti-Actin

	Immunohistochemistry: Actin (Pan) Antibody (EP184E) [NB110-55441] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle using anti-Actin (Pan)