Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Brain acetylcholinesterase, human, purified and detergent solubilized (1).
Marker:
Early Neuronal Development Marker
Species Reactivity:
Does not react with Human.
Applications:
Uses:
ELISA and Western Blot
Dilutions:
ELISA 1:4000,
Western Blot ,
Unit Size:
0.125 mg
Concentration:
1.0 mg/ml
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl
Preservative:
15mM Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa (1,2).
Liao J, Mortensen V, Noregaard-Pedersen B, Koch C, Brodbeck U (1993) Monoclonal antibodies against brain acetylcholinesterases which recognize the subunits bearing the hydrophobic anchor. Eur J Biochem 215:333-340.
Liao J, Boschetti N, Mortensen V, Jensen SP, Koch C, Norgaard-Pedersen B, Brodbeck U (1994) Characterization of salt-soluble forms of acetylcholinesterase from bovine brain. J Neurochem 63:1446-1453.
Massoulie J, Bon S (1982) The molecular forms of cholinesterase acetylcholinesterase in vertebrates. Annu Rev Neurosci 5:57-106.
Sorensen K, Gentinetta R, Brodbeck U (1982) An amphiphile-dependent form of human brain caudate nucleus acetylcholinesterase: purification properties. J Neurochem 39:1050-1060.