Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
NB 300-226 reacts with human and mouse forms of the protein. There is minimal cross-reactivity with Abeta 40 (see Notes).
Applications:
Uses:
Immunoprecipitation and Western blot. The investigator should determine the optimal working dilution for a specific application. Other applications have not yet been determined. Previous customers have reported poor signal in ELISA against the Abeta peptide.
Dilutions:
ELISA (-) Not Recommended, immunoprecipitation 1:100, Western Blot 1:500-1:1000
This product is unpurified. Concentration is not relevant.
Packaging:
Storage:
Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Preservative:
0.05% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Amyloid beta-protein (Abeta) is associated with neuronal injury and death in Alzheimer's disease. These proteins are the main components of amyloid plaques. Abeta 42 plays a bigger role in deneurogeneration and is more strongly associated with Alzheimer's disease than Abeta 40.
Detection of Abeta 42 on 5 ng of peptide per lane using NB 300-226 (lot A). Lane 1: Abeta-40, lane 2: Abeta-42, lane 3: Abeta-40 and -42 mix. Band appears at approximately 6 kDa.