Abeta 42 Antibody (NB300-226)

Abeta 42 Antibody

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Abeta 42 Antibody Summary:
Species:Hu, Mu
Applications:ELISA (-), IP, WB
Clonality:Polyclonal
Gene:APP
Purity:Whole antisera
Host:Rabbit
Specificity:Abeta 42
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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Abeta 42 Antibody Details:
Immunogen:Whole Abeta 42 peptide.
Localization:Cytoplasmic
Species Reactivity:

NB 300-226 reacts with human and mouse forms of the protein. There is minimal cross-reactivity with Abeta 40 (see Notes).

Applications:
Uses:Immunoprecipitation and Western blot. The investigator should determine the optimal working dilution for a specific application. Other applications have not yet been determined. Previous customers have reported poor signal in ELISA against the Abeta peptide.
Dilutions:ELISA (-) Not Recommended, immunoprecipitation 1:100, Western Blot 1:500-1:1000
Unit Size:0.1 ml (also available: 0.025 ml (sample size))
Concentration:This product is unpurified. Concentration is not relevant.
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Preservative:0.05% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: APP
Entrez351 (Human)
11820 (Mouse)
 
Background:

Amyloid beta-protein (Abeta) is associated with neuronal injury and death in Alzheimer's disease. These proteins are the main components of amyloid plaques. Abeta 42 plays a bigger role in deneurogeneration and is more strongly associated with Alzheimer's disease than Abeta 40.

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Working with Abeta 42

Abeta 42 Antibody Images (1)
Detection of Abeta 42 on 5 ng of peptide per lane using NB 300-226 (lot A). Lane 1: Abeta-40, lane 2: Abeta-42, lane 3: Abeta-40 and -42 mix. Band appears at approximately 6 kDa.
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Reviewed by:
Anonymous 5/18/2010

Application: Elisa
Sample Tested: Abeta42 peptide
Species: Rabbit
Sample Concentration: 1ng/mL - 100fg/mL dilutions
Lot: A2
Results: The Novus Ab did not work as expected. A possible explanation of the results is that the three capture antibodies used are not suitable for the assay.
Dilution Ratio: 1:100
Incubation Time: 1hr
Incubation Temperature: RT
Incubation Dilution Buffer: Cassein/PBS Diluent
Description: Goat-anti-rabbit antibody
Dilution Ratio: 1:1000
Method: HRP Substrate
Negative Control: No Abeta42 peptide added and Goat IgG
Spec Wavelength: 635

 

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Novus Explorer for Abeta 42 gene

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