ATM [p Ser1981] Antibody (EP1890Y)

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

ATM (ataxia telangiectasia mutated kinase) is the master regulator of the DNA double-strand break (DSB) repair pathway. This ubiquitously expressed serine/threonine protein kinase belongs to the PI3K-like family of proteins and responds to DSBs caused by oxidative and other genotoxic stresses (1). In addition to regulating the DNA damage response, ATM participates in vesicle and protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, cell cycle control, and acts as a tumor suppressor (2,3). Defects in ATM are associated with ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL), T-prolymphocytic leukemia (TPLL), and B-cell non-Hodgkin lymphomas (BNHL) including mantle cell lymphoma (MCL) and B-cell chronic lymphocytic leukemia (BCLL) (4).

The theoretical molecular weight of ATM is 350 kDa and it has 3 main domains: a FAT (focal adhesion targeting) domain (aa 1960-2566), a PI-3/PI-4 kinase catalytic domain (aa 2712-2962), and a C-terminal FAT domain (aa 3024-3056). ATM exists as a dimer or tetramer in its inactive state. Upon sensing DNA damage, the MRE11-RAD50-NBS1 (MRN) complex recruits ATM. The intricate process of ATM activation involves acetylation by KAT5/TIP60, autophosphorylation at Ser-1981, and dissociation into catalytically active monomers (5). Following activation, ATM phosphorylates multiple substrates such as p53/TP53 and Chk2 involved in DNA repair, checkpoint signaling, and the apoptosis pathway.

References

1. Paull TT. (2015) Mechanisms of ATM Activation. Annu Rev Biochem. 84:711-38. PMID: 25580527

2. Chaudhary MW and Al-Baradie RS. (2014) Ataxia-telangiectasia: future prospects. Appl Clin Genet. 7:159-167. PMID: 25258552

3. Stagni V, Cirotti C, and Barila D. (2018) Ataxia-Telangiectasia Mutated Kinase in the Control of Oxidative Stress, Mitochondria, and Autophagy in Cancer: A Maestro With a Large Orchestra. Front Oncol. 8:73. PMID: 29616191

4. Gumy-Pause F, Wacker P, and Sappino AP. (2004) ATM gene and lymphoid malignancies. Leukemia. 18(2):238-42. PMID: 14628072

5. Adamowicz M. (2018) Breaking up with ATM. J Immunol Sci. 2(1):26-31. PMID: 29652413

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.

Showing Publications 1 - 10 of 16. Show All 16 Publications. Collapse Publications.

Publications using NB110-66655	Applications	Species
Lesport E, Ferster A, Biver A, Roch B. Reduced recruitment of 53BP1 during interstrand crosslink repair is associated with genetically inherited attenuation of mitomycin C sensitivity in a family with Fanconi anemia. Oncotarget. 2018-01-09 [PMID: 29423082] (WB, Human)	WB	Human
Vermezovic J, Adamowicz M, Santarpia L et al. Notch is a direct negative regulator of the DNA-damage response Nat. Struct. Mol. Biol. 2015-04-20 [PMID: 25895060] (WB, IHC-P, Human)	WB, IHC-P	Human
Huang SM, Lu KT, Wang YC et al. ATM/ATR and SMAD3 pathways contribute to 3-indole-induced G? arrest in cancer cells and xenograft models. Anticancer Res 2011-01-01 [PMID: 21273599] (ICC/IF)	ICC/IF
Bensimon A, Schmidt A, Ziv Y et al. ATM-dependent and -independent dynamics of the nuclear phosphoproteome after DNA damage. Sci Signal 2010-12-01 [PMID: 21139141] (ICC/IF)	ICC/IF
Boichuk S, Hu L, Hein J, Gjoerup OV. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. J Virol 2010-08-01 [PMID: 20519379] (ICC/IF)	ICC/IF
White JS, Choi S, Bakkenist CJ et al. Transient ATM kinase inhibition disrupts DNA damage-induced sister chromatid exchange. Sci Signal 2010-06-01 [PMID: 20516478] (WB)	WB
Kobayashi J, Kato A, Ota Y et al. Bisbenzamidine derivative, pentamidine represses DNA damage response through inhibition of histone H2A acetylation. Mol Cancer 2010-02-01 [PMID: 20144237] (ICC/IF)	ICC/IF
Williamson CT, Muzik H, Turhan AG et al. ATM deficiency sensitizes mantle cell lymphoma cells to poly(ADP-ribose) polymerase-1 inhibitors. Mol Cancer Ther 2010-02-01 [PMID: 20124459] (ICC/IF)	ICC/IF
Pires IM, Bencokova Z, Milani M et al. Effects of acute versus chronic hypoxia on DNA damage responses and genomic instability. Cancer Res 2010-02-01 [PMID: 20103649] (ICC/IF)	ICC/IF
Noon AT, Shibata A, Rief N et al. 53BP1-dependent robust localized KAP-1 phosphorylation is essential for heterochromatic DNA double-strand break repair. Nat Cell Biol 2010-02-01 [PMID: 20081839] (ICC/IF)	ICC/IF
Gagou ME, Zuazua-Villar P, Meuth M et al. Enhanced H2AX phosphorylation, DNA replication fork arrest, and cell death in the absence of Chk1. Mol Biol Cell 2010-03-01 [PMID: 20053681] (ICC/IF)	ICC/IF
Beyrouthy MJ, Garner KM, Hever MP et al. High DNA methyltransferase 3B expression mediates 5-aza-deoxycytidine hypersensitivity in testicular germ cell tumors. Cancer Res 2009-12-01 [PMID: 19951990] (ICC/IF)	ICC/IF
Togano T, Sasaki M, Watanabe M et al. Induction of oncogene addiction shift to NF-kappaB by camptothecin in solid tumor cells. Biochem Biophys Res Commun 2009-12-01 [PMID: 19778522] (WB)	WB
Goff JP, Shields DS, Seki M et al. Lack of DNA polymerase theta (POLQ) radiosensitizes bone marrow stromal cells in vitro and increases reticulocyte micronuclei after total-body irradiation. Radiat Res 2009-08-01 [PMID: 19630521] (IP)	IP
Momcilovi? O, Choi S, Varum S et al. Ionizing radiation induces ataxia telangiectasia mutated-dependent checkpoint signaling and G(2) but not G(1) cell cycle arrest in pluripotent human embryonic stem cells. Stem Cells 2009-08-01 [PMID: 19544417] (ICC/IF)	ICC/IF
Ame JC, Fouquerel E, Gauthier LR et al. Radiation-induced mitotic catastrophe in PARG-deficient cells. J Cell Sci 2009-06-01 [PMID: 19454480] (ICC/IF)	ICC/IF
Show All 16 Publications. Collapse Publications.

Array NB110-66655

• ATM Antibodies
• ATM ELISA Kits
• ATM Proteins