ATG7 Antibody (R-161-100)
ATG7 Antibody
|
This product is fully covered under the Novus guarantee+ Policy.
Click
here for details.
|
|
Distributor Data...
|
|
anti-APG7-LIKE antibody, APG7-LIKE antibody, APG7L antibody, anti-ubiquitin-activating enzyme E1-like protein antibody, anti-hAGP7 antibody, anti-APG7-like antibody, anti-APG7L antibody, |
|
|
ATG7 Antibody Summary: | | Gene: | ATG7 |
|---|
| Purity: | Whole antisera |
|---|
| Host: | Rabbit |
|---|
| | Specificity: | IHC, WB and ELISA confirmed the specificity for ATG7. |
|---|
| Publications: | Antibody has been mentioned in at least 11 Publications. |
|---|
| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional ATG7 Products ATG7 Antibody Details: | Immunogen: | A synthetic peptide (DSTRDRTLDQQC) corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog). |
|---|
Human, rat. Other species not yet tested. |
| Uses: | Immunohistochemistry-P and Immunofluorescence |
|---|
| Dilutions: | Immunofluorescence 1:200 to 1:3000, Immunohistochemistry-Paraffin 1:200 to 1:3000 |
|---|
| Unit Size: | 0.1 ml |
|---|
| Concentration: | lyoph |
|---|
|
Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
|---|
| Buffer: | Lyophilized. Add diH20 to desired concentration. |
|---|
| Preservative: | No Preservative |
|---|
| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
|---|
Background: FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family.
This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.
|
Images (3) Reviews (0) Related Products (26) Working with ATG7
|
Jump to: Publications, Images, Ask a Question
|
Working with ATG7
ATG7 Antibody Images (3) Immunofluorescence: ATG7 Antibody [R-161-100] - Confocal microscopy on cytospin-isolated human blood cells. Neutrophils seem to be stained using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells stained for ATG7 appear in red. The cells were counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. Observations: No staining was observed using the Pre-immunisation serum. No staining was observed in HL60 cell line. |  Immunofluorescence: ATG7 Antibody [R-161-100] - Confocal microscopy on immunofluorescently detected APG7 in cytospin-isolated human white blood cells using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1: 100, incubated for 1 h at room temperature. The cells were double-stained for APG7 appearing in red and Myeloperoxidase (MPO) appearing in green. The cells were then counter stained with Hoechst Dye (blue colour). Here, the merged picture is presented. |  Western Blot: ATG7 Antibody [R-161-100] - Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using Rabbit antibody to ATG7: whole serum (R-161-100) at a dilution of 1:100 (ECL). |
|
|
|
Publications (11)ATG7 Antibody Product Specific:- Cheng H-C, Kim SR, Oo TF, et al. Akt suppresses retrograde degeneration of dopaminergic axons by inhibition of macroautophagy. J. Neurosci. 2011 Feb 9. [PMID: 21307249]
- chimura Y, et al. J. Biol. Chem. 279:40584-40592(2004). ;
more... - Klionsky D.J, et al. Dev. Cell 5:539-545(2003). ;
- Komatsu M, et al. J. Biol. Chem. 276:9846-9854(2001). ;
- Kim J, et al. Mol. Biol. Cell 10:1337-1351(1999). ;
- Harding T.M, et al. J. Cell Biol. 131:591-602(1995). ;
- Johnston M, et al. Science 265:2077-2082(1994). ;
- Mizushima N, et al. Nature 395:395-398(1998). ;
- Tanida I, et al. J. Biol. Chem. 277:13739-13744(2002). ;
- Tanida I, et al. Biochem. Biophys. Res. Commun. 292:256-262(2002). ;
- Yuan W, et al. Mol. Biol. Cell 10:1353-1366(1999). ;
|
|
|
|
Ask a Scientist
Can't find an answer to your question about ATG7? Ask us by using live
chat or by submitting your questions below:
|
Jump to: Lysates, Peptides and Proteins, Primary Antibodies, Secondary Antibodies
|
Related ProductsLysates (3)Peptides and ProteinsPrimary Antibodies (19)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
|---|
| ATG7 Antibody (EP1759Y) | NB110-55474 | Hu, Mu(-), Rt(-) | FACS, ICC, IHC-P, IP, WB | (2) | (3) | | | | ATG7 Antibody | NB600-1274 | Hu | ELISA, IF, WB | | (3) | | | | Atg7 Antibody (EPR6251) | NBP1-95872 | Hu, Mu, Rt | ICC, IF, WB | (2) | | | | | ATG7 Antibody | NBP1-89042 | Hu | IF, IHC-P, WB | (2) | | | | | ATG7 Antibody | NB110-74811 | Hu, Mu, Rt | IHC-Fr, IHC-P, WB | | (6) | | | | ... and 14 more. See All » |
Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
|---|
| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
|
|
