Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100% homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100% identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 558-569 of Human APG7 protein.
Species Reactivity:
This product reacts with human, and is believed to react with chimpanzee, mouse, rat, dog, and chicken.
Applications:
Uses:
The end user should determine optimal working dilutions. The expected band size in western blot is 78 kDa.
Dilutions:
ELISA 1:15000-1:75000,
immunofluorescence ,
Western Blot 1:500 - 1:2000
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and
vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating
enzymes.
Park,K. et al. A Human scFv Antibody against TRAIL Receptor 2 Induces Autophagic Cell Death in Both TRAIL-Sensitive TRAIL-Resistant Cancer Cells. Cancer Res., Aug 2007; 67: 7327 - 7334.