ATG7 Antibody (NB600-1274)

ATG7 Antibody

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ATG7 Antibody Summary:
Species:Hu
Applications:ELISA, IF, WB
Clonality:Polyclonal
Gene:ATG7
Purity:Affinity purified
Host:Rabbit
Specificity:Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100% homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100% identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.
Publications:Antibody has been mentioned in at least 3 Publications.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ATG7 Antibody Details:
Isotype:IgG
Immunogen:This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 558-569 of Human APG7 protein.
Species Reactivity:

This product reacts with human, and is believed to react with chimpanzee, mouse, rat, dog, and chicken.

Applications:
Uses:The end user should determine optimal working dilutions. The expected band size in western blot is 78 kDa.
Dilutions:ELISA 1:15000-1:75000, immunofluorescence , Western Blot 1:500 - 1:2000
Unit Size:0.05 mg
Concentration:1.46 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservative:0.01% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ATG7
Entrez10533 (Human)
 
Background:

Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

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Publications (3)

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Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, Secondary Antibodies

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Peptides and Proteins
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ATG7 in Context


Research Areas related to ATG7 (1):
ATG7 in the News (1):
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