ATG5 Antibody (NB300-368)
ATG5 Antibody
|
This product is fully covered under the Novus guarantee+ Policy.
Click
here for details.
|
|
Distributor Data...
|
|
anti-ATG5 antibody, anti-ATG5 antibody, anti-ASP antibody, anti-apoptosis-specific protein antibody, anti-APG5-like antibody, anti-APG5L antibody, anti-APG5 autophagy 5-like antibody, anti-APG5 antibody |
|
|
ATG5 Antibody Summary: | | Gene: | ATG5 |
|---|
| Purity: | Immunogen affinity purified |
|---|
| Host: | Chicken |
|---|
| | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional ATG5 Products ATG5 Antibody Details: | Isotype: | IgY |
|---|
| Immunogen: | Recombinant fragment, corresponding to amino acids 1-275 of Human APG5L. |
|---|
| Localization: | Cytoplasmic. Colocalizes with nonmuscle actin. |
|---|
Cross-reacts with Human, Rat and Mouse. |
| Uses: | Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Dilutions: | immunoprecipitation ,
Western Blot 1:2000,
Immunocytochemistry 1:200,
|
|---|
| Unit Size: | 0.05 mg |
|---|
| Concentration: | 1.0 mg/ml |
|---|
| Positive Controls: | 2 Positive Controls |
|---|
|
Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
|---|
| Buffer: | PBS |
|---|
| Preservative: | No Preservative |
|---|
| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
|---|
Background: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). APG5L May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
|
Images (1) Reviews (0) Related Products (33) Exploring ATG5 Working with ATG5
|
Jump to: Publications, Images, Ask a Question
|
Working with ATG5
ATG5 Antibody Images (1) Western Blot: E coli-derived recombinant protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-ATG5 ) |
|
|
|
Publications (10)ATG5 Antibody Background References:- [10] , Gr R.J.A., Milner A.E., Mustoe T., Johnson G.D., Owen D., Grant M.L., Gregory C.D. A novel protein expressed in mammalian cells undergoing apoptosis.
- [9] , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al. Generation initial analysis of more than 15,000 full-length human mouse cDNA sequences.
more... - [8] , Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., et al. The DNA sequence analysis of human chromosome 6.
- [7] , Chen Y., Piao Y.J., Jiang Y. A novel splicing isoform of human APG5.
- [6] , Hammond E.M., Brunet C.L., Johnson G.D., Parkhill J., Milner A.E., Brady G., Gregory C.D., Gr R.J.A. Homology between a human apoptosis specific protein the product of APG5, a gene involved in autophagy in yeast.
- [5] Yung,H.W., Xue,L. Tolkovsky,A.M., et al. Apoptosis-specific protein (ASP 45 kDa) is distinct from human Apg5, the homologue of the yeast autophagic gene apg5
- [4] Simonsen,A., Birkel,H.C., Gillooly,D.J., Mizushima,N., Kuma,A., Yoshimori,T., Slagsvold,T., Brech,A. Stenmark,H. Alfy, a novel FYVE-domain-containing protein associated with protein granules autophagic membranes
- [3] Suzuki,Y., Yamashita,R., Shirota,M., Sakakibara,Y., Chiba,J., Mizushima-Sugano,J., Nakai,K. Sugano,S. Sequence comparison of human mouse genes reveals a homologous block structure in the promoter regions
- [2] Pyo,J.O., Jang,M.H., Kwon,Y.K., Lee,H.J., Jun,J.I., Woo,H.N., Cho,D.H., Choi,B., Lee,H., Kim,J.H., Mizushima,N., Oshumi,Y. , et al. Essential roles of Atg5 FADD in autophagic cell death: dissection of autophagic cell death into vacuole formation cell death
- 1] Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J., Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. , et al. Transcriptome analysis of human gastric cancer
|
|
|
|
Ask a Scientist
Can't find an answer to your question about ATG5? Ask us by using live
chat or by submitting your questions below:
|
Jump to: Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
|
Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
|---|
| ATG5 Lysate | NBL1-07799 | | WB | (1) | | | | Other Positive Controls- E coli-derived recombinant protein.
|
Lysates (1)Peptides and ProteinsPrimary Antibodies (24)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
|---|
| ATG5 Antibody | NB110-53818 | Bv, Hu, Mk, Mu, Po, Rt, Xp, Ze | IF, IHC-P, WB | (3) | (9) | | | | ATG5 Antibody | R-111-100 | Hu | IF, IHC-P, WB | (3) | (2) | | | | ATG5 Antibody | R-138-500 | Hu | IF, IHC-P, WB | | (1) | | | | ATG5 Antibody | NBP1-76866 | Hu, Mu, Rt | ELISA, IHC-P, WB | (2) | | | | | ATG5 Antibody | NBP1-76992 | Hu, Mu, Rt | ELISA, IHC-P, WB | (2) | | | | | ... and 19 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
|---|
| Chicken IgY Antibody [Cy2] | NB120-6960 | Ch | FACS, ICC, IF, IHC-Fr, IHC-P | | | | | | Chicken IgG, H/L Chains Antibody [DyLight 488] | NBP1-72813 | Ch | FLISA, IF, WB | (2) | | | | | Chicken IgG, H/L Chains Antibody [DyLight 649] | NBP1-72815 | Ch | FLISA, IF, WB | (2) | | | | | Chicken IgG, H/L Chains Antibody [DyLight 680] | NBP1-72816 | Ch | FLISA, IF, WB | (2) | | | | | Chicken IgG, H/L Chains Antibody [DyLight 800] | NBP1-72817 | Ch | FLISA, IF, WB | (2) | | | | | See All » |
|
|
