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ATG5 Antibody (NB110-74821)

ATG5 Antibody

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ATG5 Antibody Summary:
Species:Hu, Mu
Applications:IHC-Fr, IHC-P, WB
Clonality:Polyclonal
Gene:ATG5
Purity:Whole antisera
Host:Rabbit
Specificity:ATG5.
Publications:Antibody has been mentioned in at least 1 Publication.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ATG5 Antibody Details:
Immunogen:A synthetic peptide as a part of human, mouse, chicken autophagy protein 5 (ATG5, ASP, APG5, APG5L, hAPG5) conjugated to an immunogenic carrier protein has been used as the immunogen.
Species Reactivity:

Human, mouse, other species not yet tested.

Applications:
Uses:IHC-P-Fr, WB 1:300 to 1: 2000. The optimal dilution should be determined by the end user.
Dilutions:Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot 1:300 to 1:2000
Unit Size:0.15 ml
Concentration:lyoph
Positive Controls:1 Positive Controls
Notes:
Reconstitute in 0.15 ml of sterile water. Centrifuge to remove any insoluble material. When reconstituting, glycerol (1:1) may be added for an additional stability.
Packaging:
Storage:Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:Lyophilized
Preservative:No Preservative
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ATG5
Entrez9474 (Human)
11793 (Mouse)
OMIMATG5_HUMAN ;
Swiss ProtQ9H1Y0 (Human)
 
Background:

ATG5 is an important element for autophagy and may also play an important role in the apoptotic process. Following conjugation to ATG12, the conjugate participates in the formation of autophagosome. While the mRNA is ubiquitously transcribed its expression is highly upregulated in apoptotic cells.
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Publications (1)

ATG5 Antibody Product Specific:

  1. Cadwell K, Liu JY, Brown SL, et al. A key role for autophagy the autophagy gene Atg16l1 in mouse human intestinal Paneth cells. Nature 2008;456(7219):259-263.
Protocols
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