ATG5 Antibody (NB110-53818)
ATG5 Antibody
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Distributor Data...
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anti-hCG_32959 antibody, anti-Homolog of S Cerevisiae autophagy 5 antibody, anti-ATG5 autophagy related 5 homolog antibody, anti-APG 5L antibody, anti-Autophagy protein 5 antibody, anti-OTTHUMP00000040507 antibody, anti-hAPG5 antibody, anti-ATG5 autophagy related 5-like antibody, anti-ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, anti-ATG5 antibody, anti-ATG 5 antibody, anti-ASP antibody, anti-apoptosis-specific protein antibody, anti-apoptosis specific protein antibody, anti-APG5-like antibody, anti-APG5L antibody, anti-APG5 antibody, anti-APG 5 antibody, |
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ATG5 Antibody Summary: | | Gene: | ATG5 |
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| Purity: | Peptide affinity purified |
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| Host: | Rabbit |
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| | Specificity: | This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing a.a. 1-79. The calpain cleaved form of ATG5 is missing a.a. 195-275. |
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| Publications: | Antibody has been mentioned in at least 9 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional ATG5 Products ATG5 Antibody Details: | Immunogen: | A synthetic peptide made to an N-terminal region (within residues 1-50) of the human ATG5 protein. [Swiss-Prot# Q9H1Y0] |
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| Localization: | Cytoplasm. Co-localizes with non-muscle actin. |
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Reacts with bovine, human, mouse, rat, cow, pig, primate, Xenopus and Zebrafish. |
| Uses: | This antibody is useful for Western blot analysis. The molecular weight of human ATG5 is ~33 kDa. |
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| Dilutions: | immunofluorescence, Immunohistochemistry-Paraffin 2.5ug/ml, Western Blot 1:500 |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 1.0 mg/ml |
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| Positive Controls: | 2 Positive Controls |
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| Conjugated Antibodies: | 5 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Buffer: | PBS plus 30% glycerol |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
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Images (3) Reviews (2) Related Products (33) Exploring ATG5 Working with ATG5
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Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
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Working with ATG5
ATG5 Antibody Images (3) Western Blot: ATG5 antibody [NB110-53818] - Detection of ATG5 in mouse wildtype ES cell lysate (Lane 1) using NB110-53818. Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). "Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center" |  Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NB110-53818] - Immunofluorescent staining of SY5Y cells using NB110-53818 at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator. |  Immunohistochemistry: ATG5 antibody [NB110-53818] - Staining of hepatocytes using NB110-53818 at 2.5ug/ml. Human liver hepatocytes 40X magnification. |
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Reviewed by:
Yu-Ting Yen 11/16/2011
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Application:
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Western Blot
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Sample Tested:
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Mouse
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Species:
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Mouse
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Dilution Ratio:
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1:1000
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Incubation Time:
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overnight
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Description:
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anti-rabbit HRP
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Reviewed by:
Lee Hsiao-Wei 12/20/2011
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Application:
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Western Blot
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Sample Tested:
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Rat Cardiomyocyte
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Species:
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Rat
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Results:
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The major band was observed, but sometimes there was a non-specific band detected around 4 KDa
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Blocking Buffer:
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5% Milk in TBST
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Blocking Time:
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1 hr.
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Blocking Temperature:
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RT
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Dilution Ratio:
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1:1000
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Incubation Time:
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O/N
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Incubation Temperature:
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4 Celsius
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Description:
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Goat anti-Rabbit
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Dilution Ratio:
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1:5000
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Publications (9)ATG5 Antibody Product Specific:- Elgendy M, Sheridan C, Brumatti G, Martin SJ. Oncogenic Ras-Induced Expression of Noxa and Beclin-1 Promotes Autophagic Cell Death and Limits Clonogenic Survival. Mol Cell. 2011 Feb 23.[PMID 21353614 ]
- Ling Y-H, Aracil M, Zou Y, et al. PM02734 Induces Caspase-Independent Cell Death Associated with Features of Autophagy, Inhibition of the Akt/mTOR Signaling Pathway, and Activation of DAP Kinase.Clin Cancer Res. 2011 Aug 15;17(16):5353-66. [PMID 21690574]
more... - Lee J-E, Oh H-A, Song H, et al. Autophagy Regulates Embryonic Survival During Delayed Implantation.Endocrinology. 2011 May;152(5):2067-75. [PMID 21363932]
- Wang RC, Levine B. Calcipotriol Induces Autophagy in HeLa Cells and Keratinocytes. J Invest Dermatol. 2011. [PMID 21228817]
- Fujimoto K, Hanson PT, Tran H, et al. Autophagy Regulates Pancreatic Beta Cell Death in Response to Pdx1 Deficiency and Nutrient Deprivation. J Biol Chem 2009;284(40):27664-27673. [PMID: 19654319]
- Lee J-A, Gao F-B. Inhibition of Autophagy Induction Delays Neuronal Cell Loss Caused by Dysfunctional ESCRT-III in Frontotemporal Dementia. J Neurosci 2009;29(26):8506-8511. [pubmed: 19571141]
- Cadwell K, Liu JY, Brown SL, et al. A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells. Nature. 2008 Nov 13;456(7219):259-63. Epub 2008 Oct 5.
- Pyo, J., Nah, J., Kim, H., et al. Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death. Autophagy. 2008 Apr 1;4(3):315-21.
- Wang, Y., R. Singh, et al. Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus. J Biol Chem. 2008 Feb 22;283 (8):4766-77. Epub 2007 Dec 11.
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Jump to: Conjugated Antibodies, Positive Controls, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ATG5 Antibody [HRP] | NB110-53818H | Bv, Hu, Mk, Mu, Po, Rt, Xp, Ze | ICC, IHC, WB | | (3) | | | | ATG5 Antibody [DyLight 488] | NB110-53818G | Bv, Hu, Mk, Mu, Po, Rt, Xp, Ze | IHC, WB | | (3) | | | | ATG5 Antibody [DyLight 550] | NB110-53818R | Bv, Hu, Mk, Mu, Po, Rt, Xp, Ze | IHC, WB | | (3) | | | | ATG5 Antibody [DyLight 650] | NB110-53818C | Bv, Hu, Mk, Mu, Po, Rt, Xp, Ze | IHC, WB | | (3) | | | | ATG5 Antibody [Biotin] | NB110-53818B | Bv, Hu, Mk, Mu, Po, Rt, Xp, Ze | IHC, WB | | (3) | | |
Positive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ATG5 Lysate | NBL1-07799 | | WB | (1) | | | | Other Positive Controls- Mouse wildtype ES cell lysate
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Lysates (1)Peptides and ProteinsPrimary Antibodies (27)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ATG5 Antibody | R-111-100 | Hu | IF, IHC-P, WB | (3) | (2) | | | | ATG5 Antibody | R-138-500 | Hu | IF, IHC-P, WB | | (1) | | | | ATG5 Antibody | NBP1-76866 | Hu, Mu, Rt | ELISA, IHC-P, WB | (2) | | | | | ATG5 Antibody | NBP1-76992 | Hu, Mu, Rt | ELISA, IHC-P, WB | (2) | | | | | ATG5 Antibody | NB300-368 | Hu, Mu, Rt | ICC, IP, WB | (1) | (10) | | | | ... and 22 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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