ATG4B Lysate (NBL1-07797)

ATG4B Lysate

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ATG4B Lysate Summary:
Applications:WB
Gene:ATG4B
Purity:Protein
Specificity:Homo sapiens ATG4 autophagy related 4 homolog B (S. cerevisiae) (ATG4B), transcript variant 2, mRNA.
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ATG4B Lysate Details:
Immunogen:The lysate was created in HEK293T cells, using plasmid ID RC216453 and based on accession number NM_178326. The protein contains a C-terminal DDK tag.
Epitope:C-terminal Myc/DDK
Lysate Details:
Type: OverexpressionLife Stage: Adult
Protein State: Native Tissue Condition: Normal
Sub-cellular Fraction: Whole
Applications:
Uses:This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Dilutions:Western Blot
TMW:42.4 kDa
Unit Size:0.1 mg
Concentration:1.0 mg/ml
Notes:
This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes. Please note that “1 mg/ml” refers to the total protein concentration and that the specific protein concentration is not determined at this time.
Packaging:
Storage:Store at -80 °C. Avoid freeze-thaw cycles.
Buffer:RIPA buffer
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ATG4B
Entrez23192 (Human)
Swiss ProtNM_178326 (Human)
 
Background:

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a.

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ATG4B Lysate Images (1)
Western Blot: ATG4B Lysate [NBL1-07797] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for ATG4B
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