ATG4B Antibody

Protein Type: Peptide Species: Rt, Hu Applications: IF, WB, IHC Clonality: Polyclonal	Gene: ATG4B Purity: IgG purified Host: Rabbit
Specificity: TISSUE SPECIFICITY: Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. SIMILARITY: Belongs to the peptidase C54 family. Publications: Antibody has been mentioned in at least 1 publication.
See All ATG4B Products See All ATG4B Related Products: Peptides and Proteins Primary Antibodies
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Immunogen:	A synthetic peptide (CLERFFDSEDED) corresponding to the C-terminus of human ATG4B protein conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from of ATG4B protein in mouse and rat.
Localization:	Accession Number: ATG4 B_HUMAN ; ATG4 B_MOUSE ; ATG4 B_RAT

Entrez:	23192 (Human)
	316640 (Rat)
Related Genes:	ANXA3, GABARAPL2, GABARAP, GABARAPL1, AP3B1, PREP, ATG5, ATG3, EGR1, MAP1LC3B, CASP3, ATG7, ATG12, ATG4C, UFM1, TRP53, CASP1, ATG4A, TP53, GSTA4, EXOSC10, PCBD1, GNAS, PNLIP, ATG4D, RPLP2, BECN1, DCTN4, KHDRBS1, NUP62, GABRG1, USP7, UFSP2, BCL2, UCHL3, CFP, PIK3C3, HOXA10, ZFYVE1, UFSP1

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.

Reactivity:	Human, rat. Other species not yet tested.

Uses:	This antibody is useful for Western Blot, Immunofluorescence and Immunohistochemistry.
Dilutions:	immunohistochemistry 1:1000-1:2000, Western Blot 1:1000-1:2000, immunofluorescence, 1:1000-1:2000
TMW:	44 kDa
Positive Controls:	1 positive controls

Buffer:	Lyophilised
Storage:	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.