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ATF4 Antibody (NB100-852)

ATF4 Antibody

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ATF4 Antibody Summary:
Species:Hu, Mu, Po, Rt
Applications:PEP-ELISA
Clonality:Polyclonal
Gene:ATF4
Purity:Immunogen affinity purified
Host:Goat
Specificity:This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ATF4 Antibody Details:
Isotype:IgG
Immunogen:Synthetic peptide with sequence EEVRKARGKKRVP, representing the C-Terminus of the human protein (residues 339-351) according to NP_001666.
Species Reactivity:

Human. Predicted cross-reactivity based on sequence homology: Mouse, Rat, Pig

Applications:
Uses:This antibody id useful for Western Blot. Approx 40kDa band seen in Raji cell lysate.
Dilutions:Peptide ELISA detection limit 1:1000
TMW:38.6 kDa
Unit Size:0.1 mg
Concentration:0.5 mg/ml
Positive Controls:1 Positive Controls
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:Tris saline containing [pH7.3] with 0.5% BSA
Preservative:0.02% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ATF4
Entrez468 (Human)
11911 (Mouse)
79255 (Rat)
Swiss ProtP18848 (Human)
 
Background:

ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).
Images (1)
  • NB 100-852 staining (1µg/ml) of Raji lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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1 Related Research Areas

Working with ATF4

1 Background References
Jump to: Publications, Images, Ask a Question

Working with ATF4

ATF4 Antibody Images (1)
NB 100-852 staining (1µg/ml) of Raji lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Publications (1)

ATF4 Antibody Background References:

  1. Tsujimoto A et al. Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I. J Virol 65:1420-6 (1991).
Protocols
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ATF4 in Context

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