Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
This product is useful for In Situ Proximity Ligation Assay (cell).
Dilutions:
Proximity Ligation Assay
Unit Size:
1 set
Concentration:
Concentration of individual antibodies may be found on the vial label.
Packaging:
Storage:
Store at -80 °C. Avoid freeze-thaw cycles.
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
This dual recognition antibody pair set comes with two antibodies, one against the ATF4 protein, and the other against the specific S245 phosphorylated site of ATF4 for use in in situ Proximity Ligation Assay (in situ PLA;). See Publication Reference below.
Protein detection using proximity-dependent DNA ligation assays. Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, Legren U. Nat Biotechnol 2002 May;20(5):473-7.
Cytokine detection by antibody-based proximity ligation. Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Legren U, Fredriksson S. Proc Natl Acad Sci U S A 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
Direct observation of individual endogenous protein complexes in situ by proximity ligation. Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, Legren U. brNat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29. Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method. Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, ersson AC, Wahlby C, Gullberg M,Botling J, Sjoblom T, Markova B, Ostman A, Legren U, Soderberg O.Mol Cell Proteomics 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.