Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Human, mouse, rat. Other species have not been tested.
Applications:
Uses:
The antibodies included in this pack are useful for Western blot, immunohistochemistry and immunoprecipitation. See individual datasheets for suggested working dilutions.
Dilutions:
Immunohistochemistry, Immunoprecipitation, Western Blot
Unit Size:
2 vials
Concentration:
Concentration of individual antibodies may be found on the vial label.
Notes:
1 vial each of: NB100-116 and NB100-101.
Packaging:
Storage:
Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:
See individual datasheets
Preservative:
0.05% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually.
The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and other transcription factors. APE/ref-1 has also been shown to control p53 activity through redox alteration. APE is linked to apoptosis, associated with thioredoxin, and altered levels of APE/ref-1 have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.
Immunocytochemistry: APE1 Antibody (13B8E5C2) [NB100-116] - APE1 Antibody (13B8E5C2) [NB100-116] - Immunocytochemical detection of APE-ref-1 in breast cancer cell line MDS231 using NB100-116.
Immunohistochemistry: APE/Ref-1 Antibodies SuperNovus Pack [NB100-909] - Immunohistochemical staining of APE-ref-1 in prostate cancer, using NB100-101
Western Blot: APE/Ref-1 Antibodies SuperNovus Pack [NB100-909] - Western analysis of APE-ref-1 in HeLa and 3T3 cell lysates, using NB100-101
Western Blot: APE1 Antibody (13B8E5C2) [NB100-116] - Western Blot on Ovarian Cancer cell lines using NB100-116.