AKT1 Antibody (NB600-590)
AKT1 [p Ser473] Antibody
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Distributor Data...
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anti-CAKT antibody; anti-Murine thymoma viral (vakt) oncogene homolog 1 antibody; anti-PKB Akt antibody; anti-PKB alpha antibody; anti-PKB antibody; anti-PKBalpha antibody; anti-PRKBA antibody; anti-RAC alpha serine/threonine protein kinase antibody; anti, |
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AKT1 [p Ser473] Antibody Summary: | | Gene: | AKT1 |
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| Purity: | Immunogen affinity purified |
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| Host: | Rabbit |
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| | Specificity: | AKT [Ser473] |
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| Publications: | Antibody has been mentioned in at least 1 Publication. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional AKT1 Products AKT1 [p Ser473] Antibody Details: | Isotype: | IgG |
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| Immunogen: | Synthetic peptide: CHFPQFSYSAS conjugated to KLH, corresponding to amino acids 468-477 of Human AKT. |
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| Localization: | Cytoplasmic and Nuclear |
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| Modification: | p Ser473 |
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Cross-reacts with Human. Not yet tested in other species. |
| Uses: | This antibody is useful for ELISA, Western blot, immunofluorescence/immunocytochemistry, immunohistochemister-Paraffin. For immunohistochemistry, no pre-treatment of sample is required. |
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| Dilutions: | ELISA,
Immunocytochemistry,
Immunofluorescence,
Immunohistochemistry-Paraffin 1:100-1:500,
Western Blot 1:200-1:1000 |
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| Unit Size: | 0.1 mg |
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| Concentration: | 1.02 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 0.02M K Phosphate with 0.15M NaCl pH7.2 |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene (referenced from entrez gene).
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Images (6) Reviews (0) Related Products (105) Exploring AKT1 Working with AKT1
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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- human breast tumor tissue
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Antibody Pairs (6)Lysates (2)Peptides and ProteinsPrimary Antibodies (55)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| AKT1 Antibody (2E11) | H00000207-M03 | Hu, Mu, Rt | ELISA, IF, IHC-P, WB | (7) | | | | | AKT1 Antibody | NB600-467 | Hu | ELISA, IF, IHC-P, WB | (4) | (1) | | | | AKT1 Antibody (Y89) | NB110-55448 | Hu, Mu, Rt | FACS, ICC, IHC-P, IP, WB | (2) | (1) | | | | AKT1 Antibody (6F11) | H00000207-M09 | Hu | ELISA, IF, WB | (6) | | | | | AKT1 [p Ser473] Antibody (EP2109Y) | NB100-79891 | Hu, Mu, Rt(-) | ICC, IHC-P, WB | (3) | (4) | | | | ... and 50 more. See All » |
RNAi (5)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody [NB600-590] - Rabbit used at a 1:100 dilution to detect AKT in normal human breast tissue. AKT is weakly phosphorylated in normal tissue in the breast. The phosphorylated AKT is clearly localized in the cytoplasm.Signal was developed using Dako's Techmate streptavidin-biotin reagents. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/images/AKT1 [p Ser473] Antibody-Immunohistochemistry-Paraffin-NB600-590-img0005.jpg "Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody [NB600-590] - Rabbit used at a 1:100 dilution to detect AKT in normal human breast tissue. AKT is weakly phosphorylated in normal tissue in the breast. The phosphorylated AKT is clearly localized in the cytoplasm.Signal was developed using Dako's Techmate streptavidin-biotin reagents. Tissue was formalin-fixed and paraffin embedded.")
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody [NB600-590] - Rabbit anti-AKT pS473 was used at a 1:100 dilution to detect AKT in human breast tumor tissue. The staining is much stronger than the weak basal level of phosphorylation in normal breast.Signal was developed using Dako's Techmate streptavidinbiotin reagents. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/images/AKT1 [p Ser473] Antibody-Immunohistochemistry-Paraffin-NB600-590-img0001.jpg "Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody [NB600-590] - Rabbit anti-AKT pS473 was used at a 1:100 dilution to detect AKT in human breast tumor tissue. The staining is much stronger than the weak basal level of phosphorylation in normal breast.Signal was developed using Dako's Techmate streptavidinbiotin reagents. Tissue was formalin-fixed and paraffin embedded.")
![Immunofluorescence: AKT1 [p Ser473] Antibody [NB600-590] - Microscopy. Confocal image using Rabbit anti-AKT pS473 at a 1:40 dilution to detect phosphorylated AKT (green)in cardiomyocytes infected with adenovirus expressing with wild-type AKT in conjunction with a texas-red conjugated phalloidin (red) to label filamentous actin.](http://images.novusbio.com/images/AKT1 [p Ser473] Antibody-Immunofluorescence-NB600-590-img0004.jpg "Immunofluorescence: AKT1 [p Ser473] Antibody [NB600-590] - Microscopy. Confocal image using Rabbit anti-AKT pS473 at a 1:40 dilution to detect phosphorylated AKT (green)in cardiomyocytes infected with adenovirus expressing with wild-type AKT in conjunction with a texas-red conjugated phalloidin (red) to label filamentous actin.")
![Western Blot: AKT1 [p Ser473] Antibody [NB600-590] - Western blot: Rabbit anti-AKT pS473 was used at a 1:200 dilution A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase was used.](http://images.novusbio.com/images/AKT1 [p Ser473] Antibody-Western Blot-NB600-590-img0003.jpg "Western Blot: AKT1 [p Ser473] Antibody [NB600-590] - Western blot: Rabbit anti-AKT pS473 was used at a 1:200 dilution A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase was used.")
