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AKT1 Antibody (NB600-467)

AKT1 Antibody

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AKT1 Antibody Summary:
Species:Hu
Applications:ELISA, IF, IHC-P, WB
Clonality:Polyclonal
Gene:AKT1
Purity:Whole antisera
Host:Rabbit
Specificity:AKT1
Publications:Antibody has at least 1 Background Reference.
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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AKT1 Antibody Details:
Isotype:IgG
Immunogen:Synthetic peptide corresponding to amino acids 460-480 of the C-terminal of human, rat, mouse and chicken AKT protein conjugated to KLH.
Localization:Cytoplasmic, and nuclear after activation by integrin linked protein kinase 1 (ILK1).
Species Reactivity:

Cross-reacts with Human. Expected to cross-react with Mouse, Rat and Chicken based on sequence homology. Not yet tested in other species.

Applications:
Uses:This antibody is useful for Western Blot, ELISA, immunofluorescence and Immunohistochemistry-Paraffin
Dilutions:ELISA 1:2000 - 1:10000, Immunofluorescence 1:100 - 1:1000, Immunohistochemistry-Paraffin 1:500 - 1:2000, Western Blot 1:500 - 1:2000
Unit Size:0.1 ml
Concentration:This product is unpurified. Concentration is not relevant.
Positive Controls:2 Positive Controls
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:0.02 M Potassium Phoshate, 0.15 M Sodium Chloride, pH 7.2 (Concentration 75 mg/mL by Refractometry)
Preservative:0.1% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: AKT1
Entrez207 (Human)
 
Background:

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation.
Images (4)
  • Immunofluorescence: AKT1 Antibody [NB600-467] - Anti-AKT was used in immunofluorescence microscopy at a 1:80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
  • Immunohistochemistry-Paraffin: AKT1 Antibody [NB600-467] - Rabbit anti-AKT was used at a 1:1,000 dilution to detect AKT by immunohistochemistry. In panel (A) normal colon tissue is stained. Panel (B) shows tumor tissue. Tissue was formalin-fixed and paraffin embedded.
  • Western Blot: AKT1 Antibody [NB600-467] - Akt (pan) and mouse anti-Akt pS473 specific antibodies followed by detection with DyLight 549 conjugated anti-rabbit IgG (green) and DyLight 649 conjugated anti-mouse IgG (red) secondary antibodies.
  • Western Blot: AKT1 Antibody [NB600-467] - Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western blot. Approximately 20 ug of an NIH/3T3 whole cell lysate was loaded on a 10% NuPage SDS-PAGE gel using MOPS buffer.

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Reviews (1)

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Kelly Smith on 11/9/2011

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Exploring AKT1

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Working with AKT1

1 Background References

1 Reviews from researchers like you
Jump to: Publications, Images, Reviews, Ask a Question

Working with AKT1

AKT1 Antibody Images (4)
Immunofluorescence: AKT1 Antibody [NB600-467] - Anti-AKT was used in immunofluorescence microscopy at a 1:80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.Immunohistochemistry-Paraffin: AKT1 Antibody [NB600-467] - Rabbit anti-AKT was used at a 1:1,000 dilution to detect AKT by immunohistochemistry. In panel (A) normal colon tissue is stained. Panel (B) shows tumor tissue. Tissue was formalin-fixed and paraffin embedded.
Western Blot: AKT1 Antibody [NB600-467] - Akt (pan) and mouse anti-Akt pS473 specific antibodies followed by detection with DyLight 549 conjugated anti-rabbit IgG (green) and DyLight 649 conjugated anti-mouse IgG (red) secondary antibodies.Western Blot: AKT1 Antibody [NB600-467] - Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western blot. Approximately 20 ug of an NIH/3T3 whole cell lysate was loaded on a 10% NuPage SDS-PAGE gel using MOPS buffer.
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Reviewed by:
Kelly Smith 11/9/2011
Application: Western Blot
Sample Tested: Mouse Spleen Lysate
Species: Mouse
 
Publications (1)

AKT1 Antibody Background References:

  1. Lawlor, M. A. Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival insulin responses. J. Cell Science 114:2903-2910.Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14.
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Jump to: Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

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AKT1 [p Thr308, p Thr309] Antibody PairDP0247HuPLA(1)

AKT1 [p Tyr315] Antibody PairDP0245HuPLA(1)

AKT1 [p Thr450] Antibody PairDP0246HuPLA(1)

AKT1 Antibody PairH00000207-AP11HuELISA(1)

AKT1 [p Ser124] Antibody PairDP0114HuPLA(4)

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Lysates (2)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
AKT1 LysateNBL1-07441WB(1)

AKT1 LysateH00000207-T01WB

Peptides and Proteins
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
AKT1 ProteinP3835HuFunc, PAGE

AKT1 Partial Recombinant ProteinH00000207-Q02HuELISA, WB(1)

AKT1 Partial Recombinant ProteinH00000207-Q01HuELISA, WB(1)

AKT1 Recombinant ProteinH00000207-P01HuELISA, WB(1)

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Primary Antibodies (55)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
AKT1 [p Ser473] AntibodyNB600-590HuELISA, ICC, IF, IHC-P, WB(6)(1)

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AKT1 Antibody (Y89)NB110-55448Hu, Mu, RtFACS, ICC, IHC-P, IP, WB(2)(1)

AKT1 Antibody (6F11)H00000207-M09HuELISA, IF, WB(6)

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RNAi (5)
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
AKT1 RNAiH00000207-R02VHu(2)(4)

AKT1 RNAiH00000207-R01VHu(2)(4)

AKT1 RNAiH00000207-R08Hu(1)(4)

AKT1 RNAiH00000207-R07Hu(1)(4)

AKT1 RNAiH00000207-R09Hu(1)(4)

Secondary Antibodies
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Rabbit IgG Antibody [HRP]NB730-HRbELISA, ICC, IHC-P, WB(5)

Rabbit IgG Antibody [FITC]NB730-FRbICC, IHC-P(1)

Rabbit IgG Antibody [Biotin]NB730-BRbELISA, ICC, IHC-P, WB

Rabbit IgG, H/L Chains Antibody [DyLight 488]NBP1-72944RbFLISA, IF, WB(3)

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AKT1 in Context

Research Areas related to AKT1 (2):
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