AKT1 Antibody (NB110-57279)
AKT1 Antibody (E45)
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anti-C AKT antibody, anti-vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody, anti-RAC Serine/Threonine Protein Kinase antibody, anti-RAC PK Alpha antibody, anti-RAC antibody, anti-RAC Alpha antibody, anti-Protein Kinase B Alpha antibody, anti-PRKBA antibody, anti-PKB antibody, anti-Oncogene AKT1 antibody, anti-MGC9965 antibody, |
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AKT1 Antibody (E45) Summary: | | Gene: | AKT1 |
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| Purity: | IgG purified |
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| Host: | Rabbit |
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| | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional AKT1 Products AKT1 Antibody (E45) Details: | Clone: | E45 |
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| Isotype: | IgG |
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| Immunogen: | A synthetic peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. |
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Reacts in Human, Mouse, Rat. Predicted to cross-react with bovine, based on sequence homology. |
| Uses: | This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 60kDa. |
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| Dilutions: | Western Blot 1:10000-50000, Immunohistochemistry-Paraffin 1:1000-1:2500, Immunocytochemistry 1:1000, Immunoprecipitation 1:100 |
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| TMW: | 60 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Akt1/PKBalpha is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBalpha is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBalpha inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBalpha regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBalpha isoforms have been identified.
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Images (2) Reviews (0) Related Products (105) Exploring AKT1 Working with AKT1
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Jump to: Publications, Images, Protocols/Procedures, Ask a Question
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Working with AKT1
AKT1 Antibody (E45) Images (2) Immunohistochemistry: Akt1/PKBa Antibody (E45) [NB110-57279] - Immunohistochemical analysis of paraffin-embedded squamous lung carcinoma using anti-Akt1/PKBa (cat. #110-57279). |  Western Blot: Akt1/PKBa Antibody (E45) [NB110-57279] - Analysis on NIH3T3 cell lysate using anti- Akt1 pan. |
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Publications (1)AKT1 Antibody (E45) Background References:- Burgering, B.M. P.J. Coffer. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376: 599
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Jump to: Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsAntibody Pairs (6)Lysates (2)Peptides and ProteinsPrimary Antibodies (85)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| AKT1 [p Ser473] Antibody | NB600-590 | Hu | ELISA, ICC, IF, IHC-P, WB | (6) | (1) | | | | AKT1 Antibody (2E11) | H00000207-M03 | Hu, Mu, Rt | ELISA, IF, IHC-P, WB | (7) | | | | | AKT1 Antibody | NB600-467 | Hu | ELISA, IF, IHC-P, WB | (4) | (1) | | | | AKT1 Antibody (Y89) | NB110-55448 | Hu, Mu, Rt | FACS, ICC, IHC-P, IP, WB | (2) | (1) | | | | AKT1 Antibody (6F11) | H00000207-M09 | Hu | ELISA, IF, WB | (6) | | | | | ... and 80 more. See All » |
RNAi (5)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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