AEC Chromogen/Substrate Kit Summary:
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| Specificity: | Peroxidase reacts with 3% Hydrogen Peroxide Substrate to degrade it, which in turn reacts with AEC to precipitate it at the positive sites giving red brown colored end product. |
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| Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional AEC Chromogen/Substrate Kit Products
| Uses: | To use as substrate/chromogen in conjunction with peroxidase based immunostaining systems. |
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| Unit Size: | 3 Vials |
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| Concentration: | Concentration is not relevant for this product. Please see the protocols for proper use of this product. |
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Notes:
Kit Components: 3mL concentrated AEC Buffer, 3mL concentrated AEC Chromogen and 3 mL of 3% Hydrogen Peroxide Substrate.
Packaging:
| Storage: | Store at 4 °C. Do not freeze. |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background:
Even though DAB is much more sensitive chromogen compared to amino ethyl carbazol (AEC) because of its carcinogenic nature, some labs avoid using DAB. To resolve this problem, we have designed an AEC chromogen in liquid format. AEC is a chromogen of choice when performing immunoperoxidase staining. It has been well accepted amongst the pathologist because of its relatively less toxic nature compared to DAP. It produces a red colored end product at the positive sites which gives a good contrast with blue hematoxylin counter stain. Specimens stained in AEC cannot be dehydrated in ethanol and hence should be mounted in aqueous based mounting medium.
