ABCG8 Antibody [DyLight 550] 0.1 ml



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Ordering Information: NB400-110R

  • Catalog Number
    NB400-110R
  • Displayed Format
    DyLight 550
  • Other Formats
  • Size(s)
    0.1 ml
  • Availability
  • Price
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ABCG8 Antibody [DyLight 550] Summary

Species Human, Mouse (Negative)
Tested Applications WB
Clonality
Polyclonal
Host
Rabbit
Gene
ABCG8
Purity
Immunogen affinity purified
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ABCG8 Antibody [DyLight 550] Details

Format
DyLight 550 (A=562, E=576)
Immunogen
A synthetic peptide from the N-terminal region of human ABCG8 protein. [UniProt# Q9H221]
Localization
Integral membrane protein; multi-pass membrane protein

Species Reactivity

Human. Does not appear to react with mouse.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
Application Notes
This ABCG8 antibody is useful for Western blot. The antibody does not appear to be applicable in immunohistochemistry.
Other Available
Formats
Biotin Labeled NB400-110B
DyLight 488 Labeled NB400-110G
DyLight 650 Labeled NB400-110C
HRP Labeled NB400-110H

Contact Information

Product PDFs

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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM sodium borate
Unit Size
0.1 ml
Concentration
1.0 mg/ml
Preservative
No Preservative
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol ABCG8
Entrez
Uniprot

Alternate Names for ABCG8 Antibody [DyLight 550]

  • ATP-binding cassette sub-family G member 8
  • ATP-binding cassette, sub-family G (WHITE), member 8
  • ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
  • GBD4ATP-binding cassette, subfamily G, member 8
  • MGC142217
  • sterolin 2
  • sterolin-2
  • Sterolin-2
  • STSL
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Related Products by Gene

Background

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. ABCG8, expressed at high levels in the liver and intestine, normally cooperates with ABCG5 to limit intestinal absorption and promote biliary excretion of sterols. The mutated form of this transporter can lead to sterol accumulation and atherosclerosis or sitosterolemia, a rare autosomal recessive disorder, characterized by hyperabsorption of sterols and the inability to excrete sterols into bile.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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FAQs for ABCG8 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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Other Available Formats

Biotin Labeled NB400-110B
DyLight 488 Labeled NB400-110G
DyLight 650 Labeled NB400-110C
HRP Labeled NB400-110H

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Bioinformatics Tool for ABCG8

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Research Areas for ABCG8

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Blogs on ABCG8

Check out the latest blog posts on ABCG8.
Understanding Sitosterolemia: How ABCG5-ABCG8 Dimer Affects Blood Sterol Levels
The ATP binding cassette (ABC) transporter family is the largest and most diverse family of membrane transport proteins and, as the name suggests, uses the energy generated by ATP hydrolysis to transport substrates across membranes. Eukaryotic ABC tra...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

Exploring how ABCG8 Affects Heart Disease
The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th...  Read full blog post.