ABCG8 Antibody (1B10A5) Summary
||WB, ICC/IF, IHC, IHC-P, IP
Protein G purified
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ABCG8 Antibody (1B10A5) Details
Full length mouse ABCG8 [Swiss-Prot# Q9DBM0]
Membrane; Multi-pass membrane protein
Mouse and human.
Read 1 rated 5 using NBP1-71706 in the following species:
||Read Publications using NBP1-71706 in the following species:
- Western Blot 1:1000
- Immunocytochemistry/Immunofluorescence 1:100
- Immunohistochemistry 1:100
- Immunohistochemistry-Paraffin 1:100
- Immunoprecipitation 25ug
This ABCG8 (clone 1B10A5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections.
Read 1 rated 5 using
NBP1-71706 in the following applications:
Read Publications using
NBP1-71706 in the following applications:
Frequently Purchased Together
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ABCG8 Antibody (NBP1-71706) has been mentioned in at least 5 publications
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Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Alternate Names for ABCG8 Antibody (1B10A5)
- ATP-binding cassette sub-family G member 8
- ATP-binding cassette, sub-family G (WHITE), member 8
- ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
- GBD4ATP-binding cassette, subfamily G, member 8
Related Products by Gene
, ATP-BINDING CASSETTE TRANSPORTER
Normal digestion is partially facilitated by the function of ABCG8 and its counterpart ABCG5, which together form a heterodimer which transports cholesterol out of the liver and into bile. Subsequently, cholesterol is eliminated from the bodies of healthy individuals. However, in cases of insulin resistance, this functionality is impaired, and digestion does not result in complete elimination of harmful cholesterol. Unsurprisingly, mutations in ABCG8 can lead to gallstone disease, biliary cancer, hypercholesterolemia and atherosclerosis. In studies related to diabetes, heart disease, and digestive cancers, ABCG8 is commonly assessed for normal expression, function and interaction with ABCG5.
Reviews for ABCG8 Antibody (NBP1-71706) (1)
Read what people are saying who have used ABCG8 Antibody (NBP1-71706).
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Western Blot.
Average Rating: 5
(Based on 1 reviews)
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|Sample Tested||See PMID 20210363|
|Blocking Details||See PMID 20210363|
|Detection Notes||See PMID 20210363|
|Comments||Independent review from antibodyresource.comPublished in PMID: 20210363.|
Publications for ABCG8 Antibody (NBP1-71706) (5)
Read what people are writing who have used ABCG8 Antibody (NBP1-71706).
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 3 applications: WB, ICC/IF, IP.
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|af GA, Yu L, Li WP, Gerard R, Tuma PL, Cohen JC, Hobbs HH. ABCG5 and ABCG8 are obligate heterodimers for protein trafficking and biliary cholesterol excretion. J Biol Chem;278(48):48275-82. 2003 Nov 28. [PMID: 14504269] (WB, Human, Mouse)
|Zhang DW, Graf GA, Gerard RD, Cohen JC, Hobbs HH. Functional asymmetry of nucleotide-binding domains in ABCG5 and ABCG8. J Biol Chem;281(7):4507-16. 2006 Feb 17. [PMID: 16352607] (WB, IP, Mouse)
|Wang J, Zhang DW, Lei Y, Xu F, Cohen JC, Hobbs HH, Xie XS. Purification and reconstitution of sterol transfer by native mouse ABCG5 and ABCG8. Biochemistry;47(18):5194-204. 2008 May 6. [PMID: 18402465] (WB, IP, ICC/IF, Mouse)
||ICC/IF, IP, WB
|Wang J, Grishin N, Kinch L, Cohen JC, Hobbs HH, Xie XS. Sequences in the nonconsensus nucleotide-binding domain of ABCG5/ABCG8 required for sterol transport. J Biol Chem;286(9):7308-14. 2011 Mar 4. [PMID: 21209088] (WB, Mouse)
|von Kampen O, Buch S, Nothnagel M et al. Genetic and functional identification of the likely causative variant for cholesterol gallstone disease at the ABCG5/8 lithogenic locus. Hepatology 2012 Aug 16 [PMID: 22898925] (ICC/IF, Human)
Product General Protocols
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FAQs for ABCG8 (3)
- I have two options for ABCG8 primary antibodies from Novus Biologicals which we can use for fluorescence microscopy. Both of them have not been used/ tested in caco-2 cells (human colon carcinoma cell line) which is our model but for the below: 1) Used for IHC-P in liver, tonsil tissues (species human) (http://www.novusbio.com/ABCG8-Antibody_NBP1-83388.html); 2) Used for IHC-P in mouse intestine (species - mouse) (http://www.novusbio.com/ABCG8-Antibody-1B10A5_NBP1-71706SS.html); Out of the above two which do you and your team think will be more applicable for our purpose.
- I have spoken to our team and we would recommend using NBP1-83388 and applying for our Innovators Reward Program. Because you are doing ICC staining and we have only tested in tissue. Since it is confirmed to react with human and stain tissue it should probably be a better candidate than testing one which has worked in mouse samples, but not been shown to detect the human form of the protein.
- In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
- For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
- I was wondering what does Format 7C mean as far as anitbodies go?
- 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)
Bioinformatics Tool for ABCG8
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