ABCG8 Antibody (1B10A5) 0.025 ml

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Western Blot: ABCG8 Antibody (1B10A5) [NBP1-71706] - Analysis of ABCG8 expression in ABCG8 lysate using NBP1-71706.
Immunohistochemistry: ABCG8 Antibody (1B10A5) [NBP1-71706] - Immunohistochemical analysis of ABCG8 in mouse intestine using NBP1-71706.
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Ordering Information: NBP1-71706

  • Catalog Number
    NBP1-71706
  • Displayed Format
    Unconjugated
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ABCG8 Antibody (1B10A5) Summary

Species Human, Mouse
Tested Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Monoclonal
Host
Mouse
Gene
ABCG8
Purity
Protein G purified
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ABCG8 Antibody (1B10A5) Details

Clone
1B10A5
Isotype
IgG
Immunogen
Full length mouse ABCG8 [Swiss-Prot# Q9DBM0]
Localization
Membrane; Multi-pass membrane protein

Species Reactivity

Mouse and human.
Reviewed Species Read 1 rated 5
using NBP1-71706 in the following species:

Publications Read Publications using NBP1-71706 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 25ug
Application Notes
This ABCG8 (clone 1B10A5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded sections.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NBP1-71706AF405
Alexa Fluor (R) 488 Labeled NBP1-71706AF488
Alexa Fluor (R) 647 Labeled NBP1-71706AF647
Alexa Fluor (R) 700 Labeled NBP1-71706AF700
Biotin Labeled NBP1-71706B
DyLight 405 Labeled NBP1-71706V
DyLight 488 Labeled NBP1-71706G
DyLight 550 Labeled NBP1-71706R
DyLight 650 Labeled NBP1-71706C
FITC Labeled NBP1-71706F
HRP Labeled NBP1-71706H
Reviewed Applications
Read 1 rated 5
using
NBP1-71706 in the following applications:

Publications
Read Publications using
NBP1-71706 in the following applications:

  • 5 publications
  • 2 publications
  • 2 publications

Contact Information

Product PDFs

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Reviews

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Publications

ABCG8 Antibody (NBP1-71706) has been mentioned in at least 7 publications.
Earn rewards if you have published using ABCG8 Antibody (NBP1-71706).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-glycine, 150 mM NaCl
Unit Size
0.025 ml
Concentration
1 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol ABCG8
Entrez
Uniprot

Alternate Names for ABCG8 Antibody (1B10A5)

  • ATP-binding cassette sub-family G member 8
  • ATP-binding cassette, sub-family G (WHITE), member 8 (sterolin 2)
  • ATP-binding cassette, sub-family G (WHITE), member 8
  • GBD4ATP-binding cassette, subfamily G, member 8
  • MGC142217
  • sterolin 2
  • sterolin-2
  • STSL
Show More

Related Products by Gene

Background

Normal digestion is partially facilitated by the function of ABCG8 and its counterpart ABCG5, which together form a heterodimer which transports cholesterol out of the liver and into bile. Subsequently, cholesterol is eliminated from the bodies of healthy individuals. However, in cases of insulin resistance, this functionality is impaired, and digestion does not result in complete elimination of harmful cholesterol. Unsurprisingly, mutations in ABCG8 can lead to gallstone disease, biliary cancer, hypercholesterolemia and atherosclerosis. In studies related to diabetes, heart disease, and digestive cancers, ABCG8 is commonly assessed for normal expression, function and interaction with ABCG5.

Reviews for ABCG8 Antibody (NBP1-71706) (1) 51

Read what people are saying who have used ABCG8 Antibody (NBP1-71706).

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Western Blot.
Average Rating: 5
(Based on 1 reviews)

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
WB Human Anonymous 12/24/2014
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 20210363
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20210363

Details

Detection NotesSee PMID 20210363

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 20210363.

Publications for ABCG8 Antibody (NBP1-71706) (7)

Read what people are writing who have used ABCG8 Antibody (NBP1-71706).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: WB, ICC/IF, IP.

Have you published using ABCG8 Antibody (NBP1-71706)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(5)
ICC/IF
(2)
IP
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(5)
All Species
Publication Applications Species
af GA, Yu L, Li WP, Gerard R, Tuma PL, Cohen JC, Hobbs HH. ABCG5 and ABCG8 are obligate heterodimers for protein trafficking and biliary cholesterol excretion. J Biol Chem;278(48):48275-82. 2003 Nov 28. [PMID: 14504269] (WB, Human, Mouse) WB Human, Mouse
Zhang DW, Graf GA, Gerard RD, Cohen JC, Hobbs HH. Functional asymmetry of nucleotide-binding domains in ABCG5 and ABCG8. J Biol Chem;281(7):4507-16. 2006 Feb 17. [PMID: 16352607] (WB, IP, Mouse) IP, WB Mouse
Wang J, Zhang DW, Lei Y, Xu F, Cohen JC, Hobbs HH, Xie XS. Purification and reconstitution of sterol transfer by native mouse ABCG5 and ABCG8. Biochemistry;47(18):5194-204. 2008 May 6. [PMID: 18402465] (WB, IP, ICC/IF, Mouse) ICC/IF, IP, WB Mouse
Wang J, Grishin N, Kinch L, Cohen JC, Hobbs HH, Xie XS. Sequences in the nonconsensus nucleotide-binding domain of ABCG5/ABCG8 required for sterol transport. J Biol Chem;286(9):7308-14. 2011 Mar 4. [PMID: 21209088] (WB, Mouse) WB Mouse
von Kampen O, Buch S, Nothnagel M et al. Genetic and functional identification of the likely causative variant for cholesterol gallstone disease at the ABCG5/8 lithogenic locus. Hepatology 2012 Aug 16 [PMID: 22898925] (ICC/IF, Human) ICC/IF Human
Xie Yan, Fung Ho Yee Joyce, Newberry Elizabeth P et al. Hepatic Mttp deletion reverses gallstone susceptibility in L-Fabp knockout mice. J Lipid Res. 2014 Jan 28 [PMID: 24474819] (Mouse) Mouse
Johnson BJ, Lee JY, Pickert A, Urbatsch IL. Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5/G8 Biochemistry. 2010 Apr 27 [PMID: 20210363] (WB, Human) WB Human

Product General Protocols

View specific protocols for ABCG8 Antibody (NBP1-71706): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for ABCG8 (1)

  1. I have two options for ABCG8 primary antibodies from Novus Biologicals which we can use for fluorescence microscopy. Both of them have not been used/ tested in caco-2 cells (human colon carcinoma cell line) which is our model but for the below: 1) Used for IHC-P in liver, tonsil tissues (species human) (http://www.novusbio.com/ABCG8-Antibody_NBP1-83388.html); 2) Used for IHC-P in mouse intestine (species - mouse) (http://www.novusbio.com/ABCG8-Antibody-1B10A5_NBP1-71706SS.html); Out of the above two which do you and your team think will be more applicable for our purpose.
    • I have spoken to our team and we would recommend using NBP1-83388 and applying for our Innovators Reward Program. Because you are doing ICC staining and we have only tested in tissue. Since it is confirmed to react with human and stain tissue it should probably be a better candidate than testing one which has worked in mouse samples, but not been shown to detect the human form of the protein.

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Bioinformatics Tool for ABCG8

Discover related pathways, diseases and genes to ABCG8. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for ABCG8

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Blogs on ABCG8

Check out the latest blog posts on ABCG8.
Understanding Sitosterolemia: How ABCG5-ABCG8 Dimer Affects Blood Sterol Levels
The ATP binding cassette (ABC) transporter family is the largest and most diverse family of membrane transport proteins and, as the name suggests, uses the energy generated by ATP hydrolysis to transport substrates across membranes. Eukaryotic ABC tra...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.

Exploring how ABCG8 Affects Heart Disease
The high prevalence of atherosclerosis in developed nations is not breaking news; it has been well established that heart disease is the leading cause of death in the United States, and it presents a major socioeconomic burden. Investigators across th...  Read full blog post.