ABCG1 Antibody (NB400-132B)
ABCG1 Antibody [Biotin]
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anti-White protein homolog ATP binding cassette transporter 8 antibody, anti-homolog of Drosophila white antibody, anti-sub family G WHITE member 1 antibody, anti-ATP-binding cassette transporter member 1 of subfamily G antibody, anti-ABC transporter 8 antibody, anti-ABC8 antibody, anti-wht1 antibody, anti-WHITE1 antibody, anti-White protein homolog antibody, anti-MGC34313 antibody, anti-ATP-binding cassette transporter 8 antibody, anti-ATP-binding cassette sub-family G member 1 antibody, anti-ATP-binding cassette antibody |
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ABCG1 Antibody [Biotin] Summary: | | Gene: | ABCG1 |
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| Purity: | Peptide affinity purified |
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| Host: | Rabbit |
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| | Specificity: | The specificity of this is for ABCG1.
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| Publications: | Antibody has been mentioned in at least 18 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional ABCG1 Products ABCG1 Antibody [Biotin] Details: | Conjugate: | Biotin |
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| Immunogen: | A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). |
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This antibody reacts with mouse and human protein. Other species have not been tested. There is 100%/100% identity/homology of the immunogen used for this production and the human protein sequence. There is 84%/89% for mouse and 78%/88% for rat. |
| Uses: | This antibody is useful for Western blot on mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) and immunohistochemistry. The theoretical molecular weight of ABCG1 is ~75.6 kDa, as seen by Western blot. Preliminary results suggest that this antibody may be also used for immunofluorescence on mouse ABCG1 overexpressed in HEK93 cells. It is not recommended for immunoprecipitation. |
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| Dilutions: | immunohistochemistry ,
Western Blot |
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| Unit Size: | 0.1 ml |
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| Concentration: | 1.0 mg/ml |
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| Conjugated Antibodies: | 5 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | PBS |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages and is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. In contrast, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1.
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Images (2) Reviews (0) Related Products (21) Exploring ABCG1 Working with ABCG1
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Jump to: Publications, Images, Ask a Question
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Working with ABCG1
ABCG1 Antibody [Biotin] Images (2) Detection of ABCG1 in mouse peritoneal macrophages (40 ug), using NB 400-132. Lane 1: positive ABCG1 control, lane 2: 10 uM 22-(R)-hydrocholesterol treated macrophages, Lane 3: 100 nM T0901713 (LXRa ligand) treated macrophages, and Lane 4: 500 nM T091713 (LXRa ligand) treated macrophages. |  Staining of lung, alveolar macrophages using NB400-132. |
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Publications (18)ABCG1 Antibody [Biotin] Product Specific:- Wang, M.-D., Kiss, R. S., Franklin, V., McBride, H. M., Whitman, S. C., Marcel, Y. L. (2007) Different cellular traffic of LDL-cholesterol acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways, 48, 633-645.
- Tansley, G. H., Burgess, B. L., Bryan, M. T., Su, Y., Hirsch-Reinshagen, V., Pearce, J., Chan, J. Y., Wilkinson, A., Evans, J., Naus, K. E., McIsaac, S., Bromley, K., Song, W., Yang, H.-C., Wang, N., DeMattos, R. B., Wellington, C. L. (2007) The cholesterol transporter ABCG1 modulates the subcellular distribution proteolytic processing of beta -amyloid precursor protein, M600542-JLR600200.
more... - Ranalletta, M., Wang, N., Han, S., Yvan-Charvet, L., Welch, C., Tall, A. R. (2006) Decreased Atherosclerosis in Low-Density Lipoprotein Receptor Knockout Mice Transplanted With Abcg1-/- Bone Marrow, 26, 2308-2315.
- Li, T., Chen, W., Chiang, J. Y. L. (2007) PXR induces CYP27A1 regulates cholesterol metabolism in the intestine, 48, 373-384.
- Burgess, B., Parkinson, P., Racke, M., et al. ABCG1 influences the brain cholesterol biosynthetic pathway but does not affect amyloid precursor protein or apolipoprotein E metabolism in vivo. J. Lipid Res.2008; 49: 1254-1267.
1. Avallone, R., Demers, A., Rodrigue-Way, A., Bujold, K., Harb, D., Anghel, S., Wahli, W., Marleau, S., Ong, H., Tremblay, A. (2006) A Growth Hormone-Releasing Peptide that Binds Scavenger Receptor CD36 Ghrelin Receptor Up-Regulates Sterol Transporters Cholesterol Efflux in Macrophages through a Peroxisome Proliferator-Activated Receptor {gamma}-Dependent Pathway, 20, 3165-3178.
- Mauldin JP, Nagelin MH, Wojcik AJ, et al. Reduced Expression of ATP-Binding Cassette Transporter G1 Increases Cholesterol Accumulation in Macrophages of Patients With Type 2 Diabetes Mellitus. Circulation. May 27, 2008;117(21):2785-92.
- Wojcik AJ, Skaflen MD, Srinivasan S, Hedrick CC. A Critical Role for ABCG1 in Macrophage Inflammation Lung Homeostasis. J Immunol. March 15, 2008;180(6):4273-82.
- Ouimet M, Wang M-D, Cadotte N, et al. Epoxycholesterol Impairs Cholesteryl Ester Hydrolysis in Macrophage Foam Cells, Resulting in Decreased Cholesterol Efflux. Arterioscler Thromb Vasc Biol 2008;28(6):1144-1150.
- Hu, W., S. Abe-Dohmae, et al. (2008). Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo. J. Lipid Res. 49: 386-393.
- Burgess, B. L., P. F. Parkinson, et al. (2008). ABCG1 influences brain cholesterol synthesis but does not affect amyloid precursor protein or apolipoprotein E metabolism in vivo. J. Lipid Res.: M700481-JLR200.
- Zhou X, He W, Huang Z, Gotto AM, Jr., Hajjar DP, Han J. Genetic Deletion of Low Density Lipoprotein Receptor Impairs Sterol-induced Mouse Macrophage ABCA1 Expression: A NEW SREBP1-DEPENDENT MECHANISM. J Biol Chem. 2008 January 25, 2008;283(4):2129-38.
- Hu, W., et al. Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo. J. Lipid Res. 49: 386-393, 2008.
- Tarr, P.T., et al. ABCG1 ABCG4 are coexpressed in neurons astrocytes of the CNS regulate cholesterol homeostatsis through SREBP-2. J. Lipid Res. 49: 169-182 (2008)[Western blot, Mouse]
- Yvan-Charvet, L., et al. SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL. J. Lipid Res. 49: 107-114 (2008)[Western blot, Mouse]
- Out, R., et al. Coexistence of Foam Cells Hypocholesterolemia in Mice Lacking the ABC Transporters A1 G1. Circ. Res. Jan 2008; 102: 113-120
- Li, Tiangang., et al. Manuscripts: PXR induces CYP27A1 regulates cholesterol metabolism in the intestine. 10.1194?jlr.M600282-JLR200, 2006.
- Wang, M., et al. quot;Different cellular traffic of LDL-cholesterol acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways.quot; Journal of Lipid Research. 48: 633-645, 2007.
- Tansley, Gavin H., et al. quot;The Cholesterol Transporter ABCG1 Modulates The Subcellular Distribution Protecolytic Processing of B-Amyloid Precursor Protein.quot; Journal of Lipid Research. doi: 10.1194/jlr.M600542-JLR200, 2007.
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Jump to: Conjugated Antibodies, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ABCG1 Antibody [DyLight 488] | NB400-132G | Hu, Mu | IHC, WB | | (22) | | | | ABCG1 Antibody [DyLight 550] | NB400-132R | Hu, Mu | IHC, WB | | (22) | | | | ABCG1 Antibody [DyLight 650] | NB400-132C | Hu, Mu | IHC, WB | | (22) | | | | ABCG1 Antibody [HRP] | NB400-132H | Hu, Mu | IHC, WB | (2) | (18) | | | | ABCG1 Antibody [HiLyte Fluor 488] | NB400-132L | Hu, Mu | IHC, WB | (2) | (18) | | |
Peptides and ProteinsPrimary Antibodies (10)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ABCG1 Antibody | NB400-132 | Hu, Mu | ICC, IF, IHC-P, WB | (2) | (29) | | | | ABCG1 Antibody (EP1366Y) | NB110-55438 | Hu, Mu, Rt | ICC, IHC-P, WB | (2) | (4) | | | | ABCG1 Antibody (2E11) | H00009619-M01 | Hu | ELISA | (1) | | | | | ABCG1 Antibody (2H8) | H00009619-M03 | Hu | ELISA | | | | | | ABCG1 Antibody [DyLight 488] | NB400-132G | Hu, Mu | IHC, WB | | (22) | | | | ... and 5 more. See All » |
RNAi (7)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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