ABCG1 Antibody 0.1ml

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1. I wonder if someone could contact me about our trying to assess ABCA1, G1 and SRB1 on freshly isolated human monocytes by FACS.
2. I wonder if someone could contact me about our trying to assess ABCA1, G1 and SRB1 on freshly isolated human monocytes by FACS.
3. Do you have any recommendation for a blocking buffer for use of a primary antibody in Western Blot?
4. Could you please provide information on how to find positive controls for your primary antibodies?

• I wonder if someone could contact me about our trying to assess ABCA1, G1 and SRB1 on freshly isolated human monocytes by FACS.
  Essentially all the links I am providing to are our best antibodies against the targets you have mentioned. They have the most publications, customer reviews, and positive feedback. We have also verified all of them except the ABCG1 antibody in FACS, although the ABCG1 has been validated in ICC, so since it has been shown to be effective at staining cells that should be a positive indicator of working in FACS as well. http://www.novusbio.com/ABCA1-Antibody_NB400-105.html; http://www.novusbio.com/ABCG1-Antibody_NB400-132.html (not tested in FACS specifically, but in ICC); http://www.novusbio.com/SR-BI-Antibody_NB400-104.html; http://www.novusbio.com/SR-BI-Antibody_NB400-101.html; To stain the cells we do recommend using the corresponding FACS dilution, or ICC dilution as a starting point. Typically going with the more concentrated dilution will be best since you have said the protein expression in the monocytes is low.

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• I wonder if someone could contact me about our trying to assess ABCA1, G1 and SRB1 on freshly isolated human monocytes by FACS.
  Essentially all the links I am providing to are our best antibodies against the targets you have mentioned. They have the most publications, customer reviews, and positive feedback. We have also verified all of them except the ABCG1 antibody in FACS, although the ABCG1 has been validated in ICC, so since it has been shown to be effective at staining cells that should be a positive indicator of working in FACS as well. www.novusbio.com/ABCA1-Antibody_NB400-105.html, www.novusbio.com/ABCG1-Antibody_NB400-132.html (not tested in FACS specifically, but in ICC), www.novusbio.com/SR-BI-Antibody_NB400-104.html, www.novusbio.com/SR-BI-Antibody_NB400-101.html, To stain the cells we do recommend using the corresponding FACS dilution, or ICC dilution as a starting point. Typically going with the more concentrated dilution will be best since you have said the protein expression in the monocytes is low.

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• Do you have any recommendation for a blocking buffer for use of a primary antibody in Western Blot?
  The blocking buffer that is often employed in our Western Blot QC validation is either 3% BSA, or 5% NFDM (Non-fat Dry Milk). In certain cases a mixture of both BSA and NFDM will be employed. Ensure that BSA is used as the blocking buffer for antibodies that target phosphorylated post translational modifications. This is to ensure that the antibody does not non-specifically bind the casein found in NFDM.

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• Could you please provide information on how to find positive controls for your primary antibodies?
  The images on our datasheets will provide detailed information on potential positive control cells and/or tissues, which have been previously tested by the lab for antibody validation. We also provide a related products tab at the top of the datasheet, which will contain useful control information where available. In addition to these tools, we recommend checking tools such as www.uniprot.org or www.proteinatlas.org to determine tissues and cell lines that highly express your protein of interest.

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