ABCG1 Antibody (NB400-132)
ABCG1 Antibody
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anti-White protein homolog ATP binding cassette transporter 8 antibody, anti-homolog of Drosophila white antibody, anti-sub family G WHITE member 1 antibody, anti-ATP-binding cassette transporter member 1 of subfamily G antibody, anti-ABC transporter 8 antibody, anti-ABC8 antibody, anti-wht1 antibody, anti-WHITE1 antibody, anti-White protein homolog antibody, anti-MGC34313 antibody, anti-ATP-binding cassette transporter 8 antibody, anti-ATP-binding cassette sub-family G member 1 antibody, anti-ATP-binding cassette antibody, |
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ABCG1 Antibody Summary: | | Gene: | ABCG1 |
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| Purity: | Peptide affinity purified |
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| Host: | Rabbit |
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| | Specificity: | The specificity of this antibody is for ABCG1.
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| Publications: | Antibody has been mentioned in at least 29 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional ABCG1 Products ABCG1 Antibody Details: | Immunogen: | A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). |
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This antibody reacts with mouse and human protein. Other species have not been tested. There is 100%/100% identity/homology of the immunogen used for this production and the human protein sequence. There is 84%/89% for mouse and 78%/88% for rat. |
| Uses: | Western blot and histochemistry. This ABCG1 antibody is useful for Western blot on mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) and immunohistochemistry. The theoretical molecular weight of ABCG1 is ~75.6 kDa, as seen by Western blot. Preliminary results suggest that this ABCG1 antibody may be also used for immunofluorescence on mouse ABCG1 overexpressed in HEK93 cells. It is not recommended for immunoprecipitation. |
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| Dilutions: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot 1:500 |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | 1.0 mg/ml |
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| Conjugated Antibodies: | 6 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | Tris-citrate pH 7.0-8.0 |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages and is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. In contrast, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1.
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Images (2) Reviews (0) Related Products (21) Exploring ABCG1 Working with ABCG1
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Jump to: Publications, Images, Protocols/Procedures, Ask a Question
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Working with ABCG1
ABCG1 Antibody Images (2) Immunohistochemistry: ABCG1 antibody [NB400-132] - IHC analysis of lung, alveolar macrophages using NB400-132. |  WB: ABCG1 antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. Ln1: positive ABCG1 control, 22-(R)-hydrocholesterol treated macrophages (2), T0901713 treated macrophages (3), and T091713 treated macrophages(4). |
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Publications (29)ABCG1 Antibody Product Specific:- Wong BXW, Kyle RA, Croft KD, et al. Modulation of Macrophage Fatty Acid Content and Composition by Exposure to Dyslipidemic Serum in Vitro. Lipids. 2011 Apr;46(4):371-80.[PMID 21286835]
- Edgel KA, McMillen TS, Wei H, et al. Obesity and weight loss result in increased adipose tissue ABCG1 expression in db/db mice. Biochim Biophys Acta. 2011 Dec 10. [PMID: 22179025]
more... - Chen J, Costa LG, Guizzetti M. Retinoic acid isomers up-regulate ATP binding cassette a1 and g1 and cholesterol efflux in rat astrocytes: implications for their therapeutic and teratogenic effects. J Pharmacol Exp Ther. 2011 Sep;338(3):870-8. [PMID 21628419]
- Ji A, Meyer JM, Cai L, et al. Scavenger receptor SR-BI in macrophage lipid metabolism. Atherosclerosis. 2011 Apr 8.[PMID 21481393]
- Uto-Kondo H, Ayaori M, Ogura M, et al. Coffee consumption enhances high-density lipoprotein-mediated cholesterol efflux in macrophages. Circ Res. 2010 Mar 5;106(4):779-87. [PMID: 20075335 ](Mouse, WB)
- Nagelin MH, Srinivasan S, Nadler JL, et al. Murine 12/15-lipoxygenase regulates ATP-binding cassette transporter G1 protein degradation through p38- and JNK2-dependent pathways. J Biol Chem. 2009 Nov 6;284(45):31303-14. [PMID: 19713213]
- Welch C, Santra MK, El-Assaad W, et al. Identification of a Protein, G0S2, That Lacks Bcl-2 Homology Domains Interacts with Antagonizes Bcl-2. Cancer Res 2009:0008-5472.CAN-0009-0128. [pubmed: 19706769]
- Sirek AS, Liu L, Naples M, et al. Insulin Stimulates the Expression of Carbohydrate Response Element Binding Protein (ChREBP) by Attenuating the Repressive Effect of Pit-1, Oct-1/Oct-2, Unc-86 Homeodomain Protein Octamer Transcription Factor-1. Endocrinology 2009;150(8):3483-3492. [pubmed: 19359385]
- Ronis MJ, et al. Dietary soy protein isolate attenuates metabolic syndrome in rats via effects on PPAR, LXR, and SREBP signaling. J Nutr. 2009 Aug;139(8):1431-8. Epub 2009 Jun 10.
- Xu G, Watanabe T, Iso Y, et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res 2009:CIRCRESAHA.109.193870. [pubmed: 19644050]
- Liu J, Huan C, Chakraborty M, et al. Macrophage Sphingomyelin Synthase 2 Deficiency Decreases Atherosclerosis in Mice. Circ Res 2009;105(3):295-303. [pubmed: 19590047]
- Ranalletta, M., et al. Decreased atherosclerosis in low-density lipoprotein receptor knockout mice transplanted with Abcg1-/- bone marrow. Arterioscler Thromb Vasc Biol. 2006 Oct;26(10):2308-15. Epub 2006 Aug 17.
- Li, T., Chen, W., Chiang, J. Y. L. PXR induces CYP27A1 and regulates cholesterol metabolism in the intestine. J Lipid Res. 2007 Feb;48(2):373-84. Epub 2006 Nov 6.
- Bujold K., et al. CD36-mediated cholesterol efflux is associated with PPARgamma activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res. 2009 Aug 1;83(3):457-64. Epub 2009 Apr 17.
- Ouvrier A, Cadet R, Vernet P, et al. LXR ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell specific manner. J Lipid Res 2009:M800657-JLR800200 [pubmed: 19395734]
- Nakanishi S, Vikstedt R, Soderlund S, et al. Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity. J Lipid Res. 2009 Feb;50(2):183-92. [pubmed: 18787236]
- Delvecchio CJ, Bilan P, Nair P, et al. LXR-induced reverse cholesterol transport in human airway smooth muscle is mediated exclusively by ABCA1. Am J Physiol Lung Cell Mol Physiol. 2008 Nov;295(5):L949-57. Epub 2008 Sep 26.
- Mauldin JP, et al. Reduced expression of ATP-binding cassette transporter G1 increases cholesterol accumulation in macrophages of patients with type 2 diabetes mellitus. Circulation. 2008 May 27;117(21):2785-92. Epub 2008 May 19.
- Wojcik AJ, et al. A critical role for ABCG1 in macrophage inflammation and lung homeostasis. J Immunol. 2008 Mar 15;180(6):4273-82
- Ouimet M, Wang M-D, Cadotte N, et al. Epoxycholesterol impairs cholesteryl ester hydrolysis in macrophage foam cells, resulting in decreased cholesterol efflux. Arterioscler Thromb Vasc Biol. 2008 Jun;28(6):1144-50. Epub 2008 Mar 27.
- Hu, W., et al. Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo. J Lipid Res. 2008 Feb;49(2):386-93. Epub 2007 Nov 21.
- Burgess, B.L., et al. ABCG1 influences the brain cholesterol biosynthetic pathway but does not affect amyloid precursor protein or apolipoprotein E metabolism in vivo. J Lipid Res. 2008 Jun;49(6):1254-67. Epub 2008 Feb 26.
- Zhou X., et al. Genetic deletion of low density lipoprotein receptor impairs sterol-induced mouse macrophage ABCA1 expression. A new SREBP1-dependent mechanism. J Biol Chem. 2008 Jan 25;283(4):2129-38. Epub 2007 Nov 20.
- Tarr, P.T., et al. ABCG1 and ABCG4 are coexpressed in neurons and astrocytes of the CNS and regulate cholesterol homeostasis through SREBP-2. J Lipid Res. 2008 Jan;49(1):169-82. Epub 2007 Oct 4.
- Yvan-Charvet, L., et al. SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL. J Lipid Res. 2008 Jan;49(1):107-14. Epub 2007 Oct 24.
- Out, R., et al. Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporters A1 and G1. Circ Res. 2008 Jan 4;102(1):113-20. Epub 2007 Oct 25.
- Wang, M., et al. Different cellular traffic of LDL-cholesterol and acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways. J Lipid Res. 2007 Mar;48(3):633-45. Epub 2006 Dec 5.
- Tansley, Gavin H., et al. The cholesterol transporter ABCG1 modulates the subcellular distribution and proteolytic processing of beta-amyloid precursor protein. J Lipid Res. 2007 May;48(5):1022-34. Epub 2007 Feb 10.
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ABCG1 Antibody [DyLight 488] | NB400-132G | Hu, Mu | IHC, WB | | (22) | | | | ABCG1 Antibody [DyLight 550] | NB400-132R | Hu, Mu | IHC, WB | | (22) | | | | ABCG1 Antibody [DyLight 650] | NB400-132C | Hu, Mu | IHC, WB | | (22) | | | | ABCG1 Antibody [Biotin] | NB400-132B | Hu, Mu | IHC, WB | (2) | (18) | | | | ABCG1 Antibody [HRP] | NB400-132H | Hu, Mu | IHC, WB | (2) | (18) | | |
Peptides and ProteinsPrimary Antibodies (11)RNAi (7)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | Rabbit IgG Antibody [Cy3] | NB120-6939 | Rb | ELISA, FACS, ICC, IF, IHC-Fr, IHC-P | | (1) | | | | See All » |
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