ABCG1 Antibody 0.1 ml

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Western Blot: ABCG1 Antibody [NB400-132] - Western blot analysis of Raw 264.7 (A), and Raw 264.7 + 10uM T09 18 hr (B) cell lysate using ABCG1 antibody at a concentration of 2 ug/ml.
Immunohistochemistry: ABCG1 Antibody [NB400-132] - Analysis of lung, alveolar macrophages.
Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in mouse peritoneal macrophages. Ln1: positive ABCG1 control, 22-®-hydrocholesterol treated macrophages (2), T0901713 treated macrophages (3), and T091713 treated macrophages(4).
Western Blot: ABCG1 Antibody [NB400-132] - Detection of ABCG1 in J774 mouse macrophages. Image provided by Dr. Angeliki Chroni of the National Centre for Scientific Research Demokritos.
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  • Catalog Number
    NB400-132
  • Displayed Format
    Unconjugated
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ABCG1 Antibody Summary

Species Human, Mouse, Rat, Hamster
Tested Applications WB, IHC, IHC-P, In vitro
Clonality
Polyclonal
Host
Rabbit
Gene
ABCG1
Purity
Immunogen affinity purified
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ABCG1 Antibody Details

Immunogen
A synthetic peptide made to an internal region of human ABCG1 (between residues 300-400). [UniProt# P45844]

Species Reactivity

Human, Mouse, Rat and Hamster.
Reviewed Species Read 2 rated 5
using NB400-132 in the following species:

Publications Read Publications using NB400-132 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunohistochemistry 1:100-1:400
  • Immunohistochemistry-Paraffin 1:100-1:400
  • In vitro assay
Application Notes
This ABCG1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot. In Western blot mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression) were tested, where a band was observed at ~90 kDa. Preliminary results suggest that this antibody does not appear to work in Immunoprecipitation.In vivo assay was reported in scientific literature.
Other Available
Formats
Alexa Fluor (R) 405 Labeled NB400-132AF405
Alexa Fluor (R) 488 Labeled NB400-132AF488
Alexa Fluor (R) 647 Labeled NB400-132AF647
Alexa Fluor (R) 700 Labeled NB400-132AF700
Biotin Labeled NB400-132B
DyLight 405 Labeled NB400-132V
DyLight 488 Labeled NB400-132G
DyLight 550 Labeled NB400-132R
DyLight 650 Labeled NB400-132C
FITC Labeled NB400-132F
HRP Labeled NB400-132H
Control Peptide
ABCG1 Peptide (NB400-132PEP)
Reviewed Applications
Read 2 Review rated 5
using
NB400-132 in the following applications:

Publications
Read Publications using
NB400-132 in the following applications:

Contact Information

Product PDFs

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Reviews

Average Rating: 5
Recent Reviews
WB Tested In Mouse
Reviewed On: 08/26/2013
Rated: 5

ABCG1 NB400-132
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Publications

ABCG1 Antibody (NB400-132) has been mentioned in at least 72 publications.
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Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-citrate/phosphate, pH 7-8
Unit Size
0.1 ml
Concentration
1 mg/ml
Preservative
0.1% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol ABCG1
Entrez
Uniprot

Alternate Names for ABCG1 Antibody

  • ABC transporter 8
  • ABC8ATP-binding cassette sub-family G member 1
  • ATP-binding cassette transporter 8
  • ATP-binding cassette, sub-family G (WHITE), member 1
  • homolog of Drosophila white
  • MGC34313
  • White protein homolog
  • white protein homolog (ATP-binding cassette transporter 8), 10ATP-binding cassette transporter member 1 of subfamily G
  • WHITE1
  • WHT1
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Related Products by Gene

Background

ABCG1 (ATP-binding cassette sub-family G member 1), a transporter protein primarily involved in macrophage lipid homeostasis, localizes to intracellular compartments associated with ER /Golgi membranes, and its expression is highest in macrophage-rich tissue such as spleen, lung, and thymus as well as in brain. ABCG1 form heterodimers with heterologous ABCG partners and functional heterodimerization with ABCG4 has been of great interest because brain cells, mainly neurons and astrocytes, depict high expression of both transporters. ABCG1 involves in the export of a variety of lipids (in conjunction with ABCA1) including cholesterol and phospholipids from macrophages to HDL. Macrophage lipid export is the first step in the reverse cholesterol transport pathway that exports cholesterol from peripheral cells to circulating lipoproteins for eventual excretion via liver. Cholesterol export to lipoprotein acceptors is compromised in macrophage foam cells, one of the early hallmarks of atherosclerosis. Moreover, ABCG1 plays role in T cells/inflammation, brain lipid homeostasis and its expression increases in macrophages of Tangier disease patients.

Reviews for ABCG1 Antibody (NB400-132) (2) 52

Read what people are saying who have used ABCG1 Antibody (NB400-132).

We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 review tested in 1: Western Blot.
Average Rating: 5
(Based on 2 reviews)

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
WB Human Anonymous 10/08/2014
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Summary

ApplicationWestern Blot
Sample TestedABCG1 transfected HEK293 cell lysate and controls (untransfected cells)
SpeciesHuman
Western Blot ABCG1 NB400-132
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Excellent
WB Mouse Angeliki Chroni 08/26/2013
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Summary

ApplicationWestern Blot
SpeciesMouse
Special ApplicationsExcellent antibody for Western Blot. I followed the protocol provided by Novus on the datasheet for NB400-132.
Pub Med ID23826352

Publications for ABCG1 Antibody (NB400-132) (72)

Read what people are writing who have used ABCG1 Antibody (NB400-132).

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 4 applications: WB, IHC, IHC-P, In vitro.

Have you published using ABCG1 Antibody (NB400-132)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(33)
IHC
(2)
IHC-P
(1)
In vitro
(1)
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Human
(15)
Mouse
(19)
Rat
(2)
Hamster
(1)
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Publication Applications Species
Zhou X et al. Genetic deletion of low density lipoprotein receptor impairs sterol-induced mouse macrophage ABCA1 expression. A new SREBP1-dependent mechanism. J Biol Chem. 283(4):2129-38. 2008 Jan 25. [PMID: 18029360]
Wojcik AJ et al. A critical role for ABCG1 in macrophage inflammation and lung homeostasis. J Immunol;180(6):4273-82. 2008 Mar 15. [PMID: 18322240]
Mauldin JP et al. Reduced expression of ATP-binding cassette transporter G1 increases cholesterol accumulation in macrophages of patients with type 2 diabetes mellitus. Circulation;117(21):2785-92. 2008 May 27. [PMID: 18490524]
Tallada Iborra R, Machado-Lima A, Castilho G et al. Advanced Glycation in macrophages induces intracellular accumulation of 7-ketocholesterol and total sterols by decreasing the expression of ABCA-1 and ABCG-1. Lipids in Health and Disease;10(1):172. 2011 Sep 29. [PMID: 21957962]
Edgel KA, McMillen TS, Wei H et al. Obesity and weight loss result in increased adipose tissue ABCG1 expression in db/db mice. Biochim Biophys Acta. 2011 Dec 10. [PMID: 22179025]
Ku CS, Park Y, Coleman SL, Lee J. Unsaturated fatty acids repress expression of ATP binding cassette transporter A1 and G1 in RAW 264. 7 macrophages. The Journal of Nutritional Biochemistry. 2011 Dec 29. [PMID: 22209005]
Ayaori M, Yakushiji E, Ogura M, Nakaya K, Hisada T, Uto-Kondo H, Takiguchi S, Terao Y, Sasaki M, Komatsu T, Iizuka M, Yogo M, Uehara Y, Kagechika H, Nakanishi T, Ikewaki K. Retinoic acid receptor agonists regulate expression of ATP-binding cassette transporter G1 in macrophages. Biochim Biophys Acta;1821(4):561-572. 2012 Feb 14. [PMID: 22353356]
Reboulleau A, Robert V, Vedie B et al. Involvement of cholesterol efflux pathway in the control of cardiomyocytes cholesterol homeostasis. Journal of Molecular and Cellular Cardiology. 2012 June 3. [PMID: 22668787] (WB, Rat) WB Rat
Out, R et al. Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporters A1 and G1. Circ Res;102(1):113-20. 2008 Jan 4. [PMID: 17967783]
Bujold K et al. CD36-mediated cholesterol efflux is associated with PPARgamma activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res;83(3):457-64. 2009 Aug 1. [PMID: 19377069]
Nagelin MH, Srinivasan S, Nadler JL et al. Murine 12/15-lipoxygenase regulates ATP-binding cassette transporter G1 protein degradation through p38- and JNK2-dependent pathways. J Biol Chem;284(45):31303-14. 2009 Nov 6. [PMID: 19713213]
Uto-Kondo H, Ayaori M, Ogura M et al. Coffee consumption enhances high-density lipoprotein-mediated cholesterol efflux in macrophages. Circ Res;106(4):779-87. 2010 Mar 5. [PMID: 20075335] (WB, Mouse) WB Mouse
Ji A, Meyer JM, Cai L et al. Scavenger receptor SR-BI in macrophage lipid metabolism. Atherosclerosis. 2011 Apr 8. [PMID: 21481393]
Corcoran MP, Lichtenstein AH, Meydani M et al. The effect of 17{beta}-estradiol on cholesterol content in human macrophages is influenced by the lipoprotein milieu. J Mol Endocrinol. 2011 Jun 6. [PMID: 21646386] (WB, Human) WB Human
Ranalletta, M et al. Decreased atherosclerosis in low-density lipoprotein receptor knockout mice transplanted with Abcg1-/- bone marrow. Arterioscler Thromb Vasc Biol;26(10):2308-15. 2006 Oct. [PMID: 16917103]
Li, T, Chen, W, Chiang, J Y L. PXR induces CYP27A1 and regulates cholesterol metabolism in the intestine. J Lipid Res;48(2):373-84. 2007 Feb. [PMID: 17088262]
Wang, M et al. Different cellular traffic of LDL-cholesterol and acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways. J Lipid Res;48(3):633-45. 2007 Mar. [PMID: 17148552]
Tansley, Gavin H et al. The cholesterol transporter ABCG1 modulates the subcellular distribution and proteolytic processing of beta-amyloid precursor protein. J Lipid Res;48(5):1022-34. 2007 May. [PMID: 17293612]
Tarr, PT et al. ABCG1 and ABCG4 are coexpressed in neurons and astrocytes of the CNS and regulate cholesterol homeostasis through SREBP-2. J Lipid Res;49(1):169-82. 2008 Jan. [PMID: 17916878]
Yvan-Charvet, L et al. SR-BI inhibits ABCG1-stimulated net cholesterol efflux from cells to plasma HDL. J Lipid Res;49(1):107-14. 2008 Jan. [PMID: 17960026]
Hu, W et al. Biogenesis of HDL by SAA is dependent on ABCA1 in the liver in vivo. J Lipid Res;49(2):386-93. 2008 Feb. [PMID: 18033752]
Burgess, BL et al. ABCG1 influences the brain cholesterol biosynthetic pathway but does not affect amyloid precursor protein or apolipoprotein E metabolism in vivo. J Lipid Res;49(6):1254-67. 2008 Jun. [PMID: 18314463]
Ouimet M, Wang M-D, Cadotte N et al. Epoxycholesterol impairs cholesteryl ester hydrolysis in macrophage foam cells, resulting in decreased cholesterol efflux. Arterioscler Thromb Vasc Biol;28(6):1144-50. 2008 Jun. [PMID: 18369155]
Nakanishi S, Vikstedt R, Soderlund S et al. Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity. J Lipid Res;50(2):183-92. 2009 Feb. [PMID: 18787236]
Delvecchio CJ, Bilan P, Nair P et al. LXR-induced reverse cholesterol transport in human airway smooth muscle is mediated exclusively by ABCA1. Am J Physiol Lung Cell Mol Physiol;295(5):L949-57. 2008 Nov. [PMID: 18820007]
Ronis MJ et al. Dietary soy protein isolate attenuates metabolic syndrome in rats via effects on PPAR, LXR, and SREBP signaling. J Nutr;139(8):1431-8. 2009 Aug. [PMID: 19515742]
Wong BXW, Kyle RA, Croft KD et al. Modulation of Macrophage Fatty Acid Content and Composition by Exposure to Dyslipidemic Serum in Vitro. Lipids;46(4):371-80. 2011 Apr. [PMID: 21286835]
Chen J, Costa LG, Guizzetti M. Retinoic acid isomers up-regulate ATP binding cassette a1 and g1 and cholesterol efflux in rat astrocytes: implications for their therapeutic and teratogenic effects. J Pharmacol Exp Ther;338(3):870-8. 2011 Sep. [PMID: 21628419]
Morales CR, Ni X, Smith CE et al. ABCA17 mediates sterol efflux from mouse spermatozoa plasma membranes. Histol Histopathol 27(3). 2012 Mar. [PMID: 22237709] (In vitro, Mouse) In vitro Mouse
Zhao JF, Ching LC, Huang YC, Chen CY, Chiang AN, Kou YR, Shyue SK, Lee TS. Molecular mechanism of curcumin on the suppression of cholesterol accumulation in macrophage foam cells and atherosclerosis. Mol Nutr Food Res;56(5):691-701. 2012 May. [PMID: 22648616] (WB, Mouse) WB Mouse
Sirek AS, Liu L, Naples M et al. Insulin Stimulates the Expression of Carbohydrate Response Element Binding Protein (ChREBP) by Attenuating the Repressive Effect of Pit-1, Oct-1/Oct-2, Unc-86 Homeodomain Protein Octamer Transcription Factor-1. Endocrinology;150(8):3483-3492. 2009 [PMID: 19359385]
Ouvrier A, Cadet R, Vernet P et al. LXR ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell specific manner. J Lipid Res :M800657-JLR800200. 2009 [PMID: 19395734] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Liu J, Huan C, Chakraborty M et al. Macrophage Sphingomyelin Synthase 2 Deficiency Decreases Atherosclerosis in Mice. Circ Res;105(3):295-303. 2009 [PMID: 19590047]
Xu G, Watanabe T, Iso Y et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res :CIRCRESAHA.109.193870. 2009 [PMID: 19644050]
Yasuyo Okada, Kiyomi Ueyama, Jyun-ichi Nishikawa, Masanori Semma, Atsushi Ichikawa. Effect of 6-O-alpha-maltosyl-beta cyclodextrin and its cholesterol inclusion complex on cellular cholesterol levels and ABCA1 and ABCG1 expression in mouse mastocytoma P-815 cells, . Carbohydrate Research, 10.1016/j.carres.2012.04.019. 2012 [PMID: 22677519] (WB, Mouse) WB Mouse
Welch C, Santra MK, El-Assaad W et al. Identification of a Protein, G0S2, That Lacks Bcl-2 Homology Domains Interacts with Antagonizes Bcl-2. Cancer Res 2009:0008-5472CAN-0009-0128. [PMID: 19706769]
Morbitzer D, Namgaladze D, Brune B. Phospholipase A(2)-Modified Low-Density Lipoprotein Activates Liver X Receptor in Human Macrophages Cell Biochem Biophys 2012 Feb 26 [PMID: 22367754] (WB, Human) WB Human
Meurs I, Calpe-Berdiel L, Habets KLL, Zhao Y, Korporaal SJA et al. Effects of Deletion of Macrophage ABCA7 on Lipid Metabolism and the Development of Atherosclerosis in the Presence and Absence of ABCA1 PLoS ONE 2012 [PMID: 22403608] (WB, Mouse) WB Mouse
Cui W, SunY ,Wang Z et al. Liver X receptor activation attenuates inflammatory response and protects cholinergic neurons in APP/PS1 transgenic mice Neuroscience Mar 5 2012 [PMID: 22425753] (WB, Mouse) WB Mouse
Tang S-L, Chen W-J, Yin K, et el. PAPP-A Negatively Regulates ABCA1, ABCG1 and SR-B1 Expression by inhibiting LXR alpha through the IGF-I-mediated signaling pathway Atherosclerosis 2012 Jun [PMID: 22503545] (WB, Human) WB Human
El Roz A, Bard JM, Huvelin JM, Nazih H. LXR Agonists and ABCG1-dependent Cholesterol Efflux in MCF-7 Breast Cancer Cells: Relation to Proliferation and Apoptosis Anticancer Res 2012 Jul [PMID: 22753765]
L Marko, G Paragh, P Ugocsai et al. Keratinocyte ATP binding cassette transporter expression is regulated by ultraviolet light Biology 2012 (WB, IHC, Human) IHC, WB Human
Beyea, M, Reaume, S, Sawyez, C et al. The Oxysterol 24(S),25-Epoxycholesterol Attenuates Human Smooth Muscle-Derived Foam Cell Formation Via Reduced Low-Density Lipoprotein Uptake and Enhanced Cholesterol Efflux J Am Heart Assoc 2012 (WB, Human) WB Human
Wang SH, Yuan SG, Peng DQ, Zhao SP. HDL and ApoA-I Inhibit Antigen Presentation-mediated T Cell Activation by Disrupting Lipid Rafts in Antigen Presenting Cells Atherosclerosis 2012 Jul 27 [PMID: 22862966] (WB, Mouse) WB Mouse
Nagashima M, Watanabe T, Terasaki M et al. Native incretins prevent the development of atherosclerotic lesions in apolipoprotein E knockout mice Diabetologia 2011 Oct [PMID: 21786155] (WB, Mouse) WB Mouse
Chen J, Zhang X, Kusumo H et al. Cholesterol efflux is differentially regulated in neurons and astrocytes: Implications for brain cholesterol homeostasis Biochim Biophys Acta 2012 Sep 23 [PMID: 23010475] (WB, Rat) WB Rat
Gelissen IC, Sharpe LJ, Sandoval C et al. Protein kinase A modulates the activity of a major human isoform of ABCG1 J Lipid Res 2012 Oct [PMID: 22872754]
Iizuka M, Ayaori M, Uto-Kondo H et al. Astaxanthin enhances ATP-binding cassette transporter A1/G1 expressions and cholesterol efflux from macrophages J Nutr Sci Vitaminol 2012 [PMID: 22790567] (WB, Mouse, Human) WB Mouse, Human
Marko L, Paragh G, Ugocsai P et al. Keratinocyte ATP binding cassette transporter expression is regulated by ultraviolet light J Photochem Photobiol B 2012 Nov 5 [PMID: 22982209] (WB, IHC, Human) IHC, WB Human
Maitra U, Li L. Molecular mechanisms responsible for the reduced expression of cholesterol transporters from macrophages by low-dose endotoxin Arterioscler Thromb Vasc Biol 2013 Jan [PMID: 23117655] (WB, Mouse) WB Mouse
Ishibashi M, Filomenko R, Rebe C et al. Knock-down of the oxysterol receptor LXRalpha impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRbeta activation Biochem Pharmacol 2013 Jan 9 [PMID: 23313547] (WB, Human) WB Human
El Roz A, Bard JM, Huvelin JM, Nazih H. The anti-proliferative and pro-apoptotic effects of the trans9,trans11 conjugated linoleic acid isomer on MCF-7 breast cancer cells are associated with LXR activation. Prostaglandins Leukot Essent Fatty Acids 2013 Jan 31 [PMID: 23375583] (WB, Human) WB Human
Gu HM, Li G, Gao X, Berthiaume LG, Zhang DW. Characterization of palmitoylation of ATP binding cassette transporter G1: effect on protein trafficking and function. Biochim Biophys Acta 2013 Feb 3 [PMID: 23388354]
Horie T, Baba O, Kuwabara Y et al. MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice. J Am Heart Assoc 2012 [PMID: 23316322] (WB, Mouse) WB Mouse
de Beer MC, Wroblewski JM, Noffsinger VP et al. The Impairment of Macrophage-to-Feces Reverse Cholesterol Transport during Inflammation Does Not Depend on Serum Amyloid A. J Lipids 2013 [PMID: 23431457] (WB, Mouse) WB Mouse
Khan OM, Akula MK, Skalen K et al. Targeting GGTase-I Activates RHOA, Increases Macrophage Reverse Cholesterol Transport, and Reduces Atherosclerosis in Mice. Circulation 2013 Feb 19 [PMID: 23334894] (WB, Mouse) WB Mouse
Mulay V, Wood P, Manetsch M et al. Inhibition of Mitogen-Activated Protein Kinase Erk1/2 Promotes Protein Degradation of ATP Binding Cassette Transporters A1 and G1 in CHO and HuH7 Cells. PLoS One 2013 Apr 25 [PMID: 23634230] (WB, Hamster, Human) WB Hamster, Human
Doddapattar P, Radovic B, Patankar JV et al. Xanthohumol ameliorates atherosclerotic plaque formation, hypercholesterolemia, and hepatic steatosis in ApoE-deficient mice. Mol Nutr Food Res 2013 May 3 [PMID: 23650230] (WB, Mouse) WB Mouse
Huang CX, Zhang YL, Wang Jf et al. MCP-1 impacts RCT by repressing ABCA1, ABCG1, and SR-BI through PI3K/Akt posttranslational regulation in HepG2 cells. J Lipid Res 2013 May [PMID: 23402987]
Fang L, Choi SH, Baek JS et al. Control of angiogenesis by AIBP-mediated cholesterol efflux. Nature 2013 Jun 6 [PMID: 23719382] (WB, Human) WB Human
Nakaya K, Ayaori M, Uto-Kondo H et al. Overexpression of stearoyl-coenzyme A desaturase 1 in macrophages promotes reverse cholesterol transport. Biochim Biophys Acta 2013 Aug [PMID: 23747827] (WB, Mouse) WB Mouse
Daniil G, Zannis VI, Chroni A. Effect of apoA-I Mutations in the Capacity of Reconstituted HDL to Promote ABCG1-Mediated Cholesterol Efflux. PLoS One 2013 Jun 27 [PMID: 23826352] (WB, Mouse, Human) WB Mouse, Human
Truong TQ, Aubin D, Falstrault L et al. SR-BI, CD36, and caveolin-1 contribute positively to cholesterol efflux in hepatic cells. Cell Biochem Funct 2010 Aug [PMID: 20629037]
Nicod N, Parker RS. Vitamin E Secretion by Caco-2 Monolayers to APOA1, but Not to HDL, Is Vitamer Selective. J Nutr. 2013 Oct [PMID: 23946344] (WB, Human) WB Human
Zhou MS, Chadipiralla K, Mendez AJ et al. Nicotine potentiates proatherogenic effects of oxLDL by stimulating and upregulating macrophage CD36 signaling. Am J Physiol Heart Circ Physiol. 2013 Aug [PMID: 23748423] (WB, Human) WB Human
Yu F, Du F, Wang Y et al. Bone Marrow Deficiency of MCPIP1 Results in Severe Multi-Organ Inflammation but Diminishes Atherogenesis in Hyperlipidemic Mice. PLoS One. 2013 Nov 6 [PMID: 24223214] (WB, Mouse) WB Mouse
Okabe A, Urano Y, Itoh S et al. Adaptive responses induced by 24S-hydroxycholesterol through liver X receptor pathway reduce 7-ketocholesterol-caused neuronal cell death. Redox Biol 2013 Dec 4 [PMID: 24371802] (WB, Human) WB Human
Du RH, Qin SY, Shi LS et al. Fumigaclavine C activates PPARgamma pathway and attenuates atherogenesis in ApoE-deficient mice. Atherosclerosis 3/5/2014 [PMID: 24637412] (WB, Mouse) WB Mouse
Tai LM, Koster KP, Luo J et al. Amyloid-b Pathology and APOE Genotype Modulate Retinoid X Receptor Agonist Activity in vivo. J Biol Chem. 2014 Sep 12 [PMID: 25217640]
Frisdal E, Lay SL, Hooton H et al. Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity. Diabetes. 2014 Sep 23 [PMID: 25249572]
Kuo CH, Leon L, Chung EJ, Huang RT. Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles. Journal of Materials Chemistry B. 2014 Sep 05
Miroshnikova VV, Demina EP, Mayorov NV. The expression of ABCG1 transporter gene in peripheral blood mononuclear cells of patients with atherosclerosis. Cell and Tissue Biology. 2014

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FAQs for ABCG1 (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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NBP1-59949
Western Blot: ATP6V0A1 Antibody [NBP1-59949] - Hela cell lysate, concentration 0.2-1 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB

NBP2-03934
Western Blot: RFC2 Antibody (4C11) [NBP2-03934] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC2.Immunohistochemistry-Paraffin: RFC2 Antibody (4C11) [NBP2-03934] Staining of paraffin-embedded Human tonsil using anti-RFC2 mouse monoclonal antibody.Flow Cytometry: RFC2 Antibody (4C11) [NBP2-03934] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RFC2 antibody, and then analyzed by flow cytometry.

Mouse Monoclonal
Species Human
Applications IHC-P, IHC, FLOW...MORE

AF6457

Sheep Polyclonal
Species Human
Applications WB

NBP1-33083
Western Blot: RFC4 Antibody [NBP1-33083] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.Immunocytochemistry/Immunofluorescence: RFC4 Antibody [NBP1-33083] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.Immunohistochemistry-Paraffin: RFC4 Antibody [NBP1-33083] - Paraffin-embedded MDAMB-468 Xenograft, using antibody at 1:100 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications IHC-P, IHC, ICC/IF...MORE

NB400-144
Western Blot: CD36 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug).  Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.Immunocytochemistry/Immunofluorescence: CD36 Antibody [NB400-144] - CD36 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Immunohistochemistry-Paraffin: CD36 Antibody [NB400-144] - Staining of human adipocytes

Rabbit Polyclonal
Species Human, Mouse, Bovine...MORE
Applications IHC-P, IHC-Fr, IHC...MORE

     1 Review

23 Publications
NB110-60531
Western Blot: ApoE Antibody (WUE-4) [NB110-60531] - 50 ug protein per lane for liver lysate and 0.5 ul of plasma at 9% SDS. Samples are loaded onto the gel as the following order: human liver lysate, mouse liver lysate, rat liver lysate, human plasma, and mouse plasma. Image from confirmed customer review.Flow Cytometry: ApoE Antibody (WUE-4) [NB110-60531] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ApoE antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.Western Blot: ApoE Antibody (WUE-4) [NB110-60531] - Detection of ApoE in human tissue lysate using NB110-60531. Lane 1: liver Lane 2: brain

Mouse Monoclonal
Species Human, Mouse, Rat (Negative)
Applications IP, IHC, FLOW...MORE

     1 Review

4 Publications
NBP1-45478
Western Blot: CETP Antibody [NBP1-45478] - Analysis of CETP expression in lysate from mouse small intestine (Lane 1) and

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications IHC, WB

NBP2-22124
Western Blot: ABCG2 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in 1) human small intestine, 2) human placenta and 3) mouse placenta tissue extracts.Immunocytochemistry/Immunofluorescence: ABCG2 Antibody (3G8) [NBP2-22124] - Immunofluorescence analysis of Hela cells using ABCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Immunohistochemistry-Paraffin: ABCG2 Antibody (3G8) [NBP2-22124] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.

Mouse Monoclonal
Species Human, Mouse, Primate
Applications WB, ELISA, FLOW...MORE

NBP2-23488
Western Blot: ABCG5 Antibody (1B5E10) [NBP2-23488] - Western blot analysis of ABCG5 in (1) HL7702, (2) RAJI and (3) Jurkat cell lysate.Immunohistochemistry: ABCG5 Antibody (1B5E10) [NBP2-23488] - IHC analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.Flow Cytometry: ABCG5 Antibody (1B5E10) [NBP2-23488] - Flow cytometry analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red).

Mouse Monoclonal
Species Human
Applications ICC/IF (-), WB, ELISA...MORE

NB600-582
Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Western blot analysis of SREBP1 expression in HeLa lysate using NB600-582.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB

12 Publications
NBP2-15229
Western Blot: ABCG4 Antibody [NBP2-15229] - Sample (30 ug of whole cell lysate) A: MCF-7 7.5% SDS PAGE; antibody diluted at 1:5000.

Rabbit Polyclonal
Species Human
Applications WB

     1 Review

NBP1-78159
Immunocytochemistry/Immunofluorescence: LDL Receptor Antibody (C7) [NBP1-78159] - Image from a customer review on porcine intestinal epithelial cells IPEC-J2.Immunohistochemistry: LDL Receptor Antibody (C7) [NBP1-78159] - Analysis of LDL Receptor on human liver.Immunocytochemistry/Immunofluorescence: LDL Receptor Antibody (C7) [NBP1-78159] - Antibody was tested in HepG2 cells with FITC (green). Nuclei were counterstained with Dapi (blue).

Mouse Monoclonal
Species Human, Bovine, Porcine
Applications WB, ELISA, ICC/IF...MORE

     1 Review

6 Publications
NBP1-30032
Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.

Mouse Monoclonal
Species Human, Mouse, Bovine...MORE
Applications WB, IHC, IHC-Fr

2 Publications

Bioinformatics Tool for ABCG1

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Research Areas for ABCG1

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Blogs on ABCG1

Check out the latest blog posts on ABCG1.
ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

ABCG1: Easy as 123
ABCG1 (ATP-binding cassette sub-family G member 1) is a transporter protein that is primarily involved in macrophage lipid homeostasis. It is a member of the superfamily of ATP-binding cassette (ABC) transporters and localizes to intracellular compart...  Read full blog post.

ABCG2: A Tumor Protector
ABCG2 is a member of the ATP-binding cassette (ABC) transporter superfamily. Among ABC transporters ABCG2 is particularly interesting for its potential role in protecting cancer stem cells and its complex oligomeric structure (1). The ABC transporters...  Read full blog post.