ABCA7 Antibody (NB400-163)

ABCA7 Antibody (7A1-144)

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ABCA7 Antibody (7A1-144) Summary:
Species:Mu
Applications:FACS, WB
Clonality:Monoclonal
Gene:ABCA7
Purity:Protein G purified
Host:Rat
Specificity:This is specific for ABCA7.
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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ABCA7 Antibody (7A1-144) Details:
Clone:7A1-144
Isotype:IgG2a Kappa
Immunogen:Mouse ABCA7 stably-transfected HeLa cells.
Localization:Cell Membrane and Cytoplasmic
Epitope:Extracellular determinants on ABCA7 of an unknown position.
Species Reactivity:

Mouse.

Applications:
Uses:By Western Blot a 240 kDa band is seen. NB 400-163 is not reactive in ELISA.
Dilutions:Flow Cytometry 1:500 - 1:4,000 , Western Blot 1:2,000
Unit Size:0.1 ml (also available: 0.025 ml (sample size))
Concentration:1.3 mg/ml
Conjugated Antibodies:5 Conjugated Antibodies
Packaging:
Storage:Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Buffer:Tris-glycine with 150mM NaCl
Preservative:0.05% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: ABCA7
Entrez10347 (Human)
27403 (Mouse)
Q91V24 (Mouse)
 
Background:

ABCA7, an ATP-binding cassette, sub-family A (ABC1), member 7, is highly homologous to ABCA1 and mediates cellular cholesterol and phospholipid release by apolipoproteins. The ABCA7 gene is regulated by sterol in the opposite direction to ABCA1 through SRE/SREBP2. The expression of ABCA7 by this regulation is associated with phagocytic activity. As well, it binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells and may mediate cholesterol efflux.

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Working with ABCA7

ABCA7 Antibody (7A1-144) Images (2)
FACS analysis of 6C (red) (negative control HeLa cells) and A7 (blue) (ABCA7 expressing HeLa cells) using NB 400-163 at a 1:2,000 dilution.Detection of ABCA7 using NB 400-163 at various dilutions in ABCA7 transfected HeLa lysates.
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Jump to: Conjugated Antibodies, Primary Antibodies, RNAi, Secondary Antibodies

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ABCA7 in Context


Research Areas related to ABCA7 (10):
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