Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Partial peptide sequence (peptide used resides somewhere between a.a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information.
Localization:
Astrocyte Marker
Marker:
Astrocyte Marker
Species Reactivity:
NB 400-105 reacts with human, rat, and mouse ABCA1. Other species have not been tested.
Applications:
Uses:
NB 400-105 may be used for Western analysis where a band representing ABCA1 is seen at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Other applications have been reported in literature using this antibody including immunofluorescence, immunohistochemistry, flow cytometry, and immunocytochemistry. NOTE: It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel.
Dilutions:
Flow Cytometry ,
immunofluorescence ,
immunoprecipitation ,
Western Blot 1:500,
Immunocytochemistry ,
Immunohistochemistry-Paraffin 1:200
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
Pou J, Rebollo A, Roglans N, et al. Ritonavir Increases CD36, ABCA1 CYP27 Expression in THP-1 Macrophages. Experimental Biology Medicine 2008;233(12):1572-1582.
Cartagena, C., Ahmed, F., Burns, M., et al. Cortical Injury Increases Cholesterol 24S Hydroxylase(Cyp46) Levels in the Rat Brain. J. of Neurotrauma. Sept. 2008:1087-1098.
Robichaud JC, Francis GA, Vance DE. A Role for Hepatic Scavenger Receptor Class B, Type I in Decreasing High Density Lipoprotein Levels in Mice That Lack Phosphatidylethanolamine N-Methyltransferase. J Biol Chem 2008;283(51):35496-35506.
Delvecchio CJ, Bilan P, Nair P, et al. LXR-Induced Reverse Cholesterol Transport in Human Airway Smooth Muscle is Mediated Exclusively by ABCA1. Am J Physiol Lung Cell Mol Physiol 2008:90394.92008.
Paul, V., Meyer, H., Leidl, K., et al. A Novel Enzyme Immunoassay Specific for ABCA1 Protein Quantification in Human Tissues Cells. J. Lipid Res. October 2008; 49: 2259-2267.
Peng D, Hiipakka RA, Dai Q, et al. Antiatherosclerotic Effects of a Novel Synthetic Tissue-Selective Steroidal Liver X Receptor Agonist in Low-Density Lipoprotein Receptor-Deficient Mice. J Pharmacol Exp Ther 2008;327(2):332-342.
Mukhamedova N, Escher G, D';Souza W, et al. Enhancing apolipoprotein A-I-dependent cholesterol efflux elevates cholesterol export from macrophages in vivo. J Lipid Res 2008;49(11):2312-2322.
Tancevski I, Wehinger A, Demetz E, et al. Reduced Plasma High-Density Lipoprotein Cholesterol in Hyperthyroid Mice Coincides with Decreased Hepatic Adenosine 5';-Triphosphate-Binding Cassette Transporter 1 Expression. Endocrinology 2008;149(7):3708-3712.
Kumar R, McClain D, Young R, et al. Cholesterol transporter ATP-binding cassette A1 (ABCA1) is elevated in prion disease affects PrPC PrPSc concentrations in cultured cells. J Gen Virol 2008;89(6):1525-1532.
Hastie C, Masters JR, Moss SE, Naaby-Hansen S. Interferon-{gamma} Reduces Cell Surface Expression of Annexin 2 Suppresses the Invasive Capacity of Prostate Cancer Cells. J Biol Chem. May 2, 2008;283(18):12595-603.
Bowden K Ridgway ND. OSBP Negatively Regulates ABCA1 Protein Stability. J Biol Chem 2008;283(26):18210-18217.
Frank PG, Pavlides S, Cheung MWC, et al. Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physiol 2008;295(1):C242-248.
Montoudis, A. et al. Intestinal fatty acid binding protein regulates mitochonfrion beta-oxidation cholesterol uptake. J. Lipid Res. January 30, 2008; Volume 49: 961-972. (WB)
Smoak K, Madenspacher J, Jeyaseelan S, et al. Effects of Liver X Receptor Agonist Treatment on Pulmonary Inflammation Host Defense. J Immunol. March 1, 2008;180(5):3305-12.
Ouimet M, Wang M-D, Cadotte N, et al. Epoxycholesterol Impairs Cholesteryl Ester Hydrolysis in Macrophage Foam Cells, Resulting in Decreased Cholesterol Efflux. Arterioscler Thromb Vasc Biol 2008;28(6):1144-1150. (Western Blot)
Watanabe, T., K. Nishio, et al. Impact of Salusin-{alpha} -{beta} on Human Macrophage Foam Cell Formation Coronary Atherosclerosis. Circulation. 2008; 117: 638-648 p.
Henry-Berger J, Mouzat K, Baron S, et al. Endoglin (CD105) Expression Is Regulated by the Liver X Receptor Alpha (NR1H3) in Human Trophoblast Cell Line JAR. Biol Reprod 2008;78(6):968-975.
Zhou X, He W, Huang Z, Gotto AM, Jr., Hajjar DP, Han J. Genetic Deletion of Low Density Lipoprotein Receptor Impairs Sterol-induced Mouse Macrophage ABCA1 Expression: A NEW SREBP1-DEPENDENT MECHANISM. J Biol Chem. January 25, 2008; 283(4):2129-38.
Yan D, Mayranpaa MI, Wong J, et al. OSBP-related Protein 8 (ORP8) Suppresses ABCA1 Expression Cholesterol Efflux from Macrophages. J Biol Chem. January 4, 2008; 283(1):332-40.
Jacobs RL, Lingrell S, Zhao Y, Francis GA, Vance DE. Hepatic CTP:Phosphocholine Cytidylyltransferase-{alpha} Is a Critical Predictor of Plasma High Density Lipoprotein Very Low Density Lipoprotein. J Biol Chem. January 25, 2008; 283(4):2147-55.
Hastie C, Masters JR, Moss SE, Naaby-Hansen S. INFgamma reduces cell surface expression of annexin 2 suppresses the invasive capacity of prostate cancer cells. J Biol Chem. January 22, 2008:M800189200.
Zhu M, Fu Y, Hou Y, et al. Laminar Shear Stress Regulates Liver X Receptor in Vascular Endothelial Cells. Arterioscler Thromb Vasc Biol. March 1, 2008;28(3):527-33. (Western Blot)
Wang N, Yvan-Charvet L, Lutjohann D, et al. ATP-binding cassette transporters G1 G4 mediate cholesterol desmosterol efflux to HDL regulate sterol accumulation in the brain. FASEB J. 2007 November 26, 2007:fj.07-9944com.
Out, R., et al. Coexistence of Foam Cells Hypocholesterolemia in Mice Lacking the ABC Transporters A1 G1. Circ. Res. Jan 2008; 102: 113-120.
Babaev, V. R., H. Ishiguro, et al. (2007). Macrophage Expression of Peroxisome Proliferator Activated Receptor-{alpha} Reduces Atherosclerosis in Low-Density Lipoprotein Receptor Deficient Mice. 116: 1404-1412.
Bates, S. R., J.-Q. Tao, et al. (2007). Expression Biological Activity of ABCA1 in Alveolar Epithelial Cells: 2007-0020OC.
Talukdar, S., et al. Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-alpha gene transcription by the liver X receptor agonist TO-901317. J. Lipid Res., Dec 2007; 48:2647-2663.
Hoe, H., et al.The Metalloprotease Inhibitor TIMP-3 Regulates Amyloid Precursor Protein Apolipoprotein E Receptor Proteolysis. J. Neurosci.27: 10895-10905.Oct 2007.
Hassan, H. et al. Identification of an ABCA1-dependent phospholipid-rich plasma membrane apolipoprotein A-1 binding site for nascent HDL formation: implications for current models of HDL biogenesis. Journal of Lipid Research. 48: 2428-2442, 2007.
During, A., et al. Mechanisms of provitamin A (carotenoid) vitamin A (retinol) transport into out of intestinal Caco-2 cells. Journal of Lipid Research. 48: 2283-2294, 2007.
Atshaves, B., et al. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. Journal of Lipid research. 48: 2193-2211, 2007.
Gaus, K., et al. Visualizing lipid structure raft domains in living cells with two-photon microscopy. PNAS 100:15554-15559, 2003.
Gerbod-Giannone, M-C. et al. TNFa induces ABCA1 through NF-kappaB in macrophage in phagocytes ingesting apoptotic cells. PNAS. 103:3112-3117, 2006.
Trogan, E., et al. Gene expression changes in foam cells the role of chemokine receptor CCR7 during athersclerosis regerssion in Apo-E-deficient mice. PNAS. 103:3781-3786, 2006.
Reddy, S.T., et al. ATP-binding cassette transporter 1 participates in LDL oxidation by artery wall cells. 22:1877-1883, 2002.
Forcheron, F., et al. Genes of cholesterol metabolism in human atheroma: overexpression of perilipin genes promoting cholesterol storage repression of ABCA1 expression. . Thromb. Vasc. Biol. 25:1711-1717, 2005.
Duong. P.T., et al. Characterization of nascent HDL particles microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J. Lipid Res. 47:832-843, 2006.
Dong, J., et al. Adenovirus-mediated overexpression of sphingomyelin synthases 1 2 increases the atherogenic potenial in mice. J. Lipid Res. 47:1307-1314, 2006.
Zhang, C., et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J. Lipid Res. 2006.
Berfield, A.K., et al. IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride. Am. J. Physiol. Renal Physiol. 290:138-147, 2006.
Zager, R.A., et al. Proximal tubular cholesterol loading after mitochondrial but not glycolytic blockade. Am. J. Physiol. Renal Physiol. 285:1092-1099, 2003.
Dong, J. Adenovirus-mediated overexpression of sphingomylein synthases 1 2 increases the atherogenic potential in mice. Journal of Lipid Research Volume 47, 2006.
Khovidhunkit, W. et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research. 44: 1728-1736, 2003. [WB, mouse]
Schmid, K., et al. Transport of cholesterol across a BeWo cell monolayer: implications for net transport of sterol from maternal to fetal circulation. Journal of Lipid Research. 44: 1909-1918, 2003. [WB, human]
Su, Y., et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice Up-regulates ABC Transporters. Molecular Therapy. 0:1-8,2003. [WB, mouse]
Glaros, E.N., et al. Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-1 pathway. J. Biol. Chem. 280(26): 24515-24523, 2005. [WB, human]
Zeng, L., et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J. Biol. Chem. 279: 48801-48807, 2004. [WB, human]
O'Connell B, et al. Cellular Physiology of Cholesterol Efflux in Vascular Endothelial cells. Circulation. 110: 2881-2888, 2004.
Agassian, M., et al. Oxysterols Trigger ABCA1-Mediated Basolateral Surfactant Efflux. Am. J. Respir. Cell Mol. Biol. 31: 227-233, 2004.
Dove, D. ACAT1 Deficiency Disrupts Cholesterol Efflux Alters Cellular Morphology in Macrophages. Arterioscler. Thromb. Vasc. Biol. 25: 128-134, 2004.
Bared, S., et al. Association of ABCA1 with Syntaxin 13 Flotillin-1 Enhanced Phagocytosis in Tangier Cells. Mol. Biol. Cell. 15: 5399-5407, 2004.
Haidar, B., et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J. Biol. Chem. 279: 9963-9969, 2004. [IP, human]
Li. A., et al. Differential inhibition of macrophage foam-cell formation atherosclerosis in mice by PPAR alpha alpha, B/delta, gamma. J. Clin. Invest. 114: 1564-1576, 2004.
o, H., et al. Effects of Pravastatin on the Expression of ATP-Binding Cassette Transporter A1. J. Pharmacol. Exp. Ther. 311: 420-425, 2004.
Koldamova, R., et al. The Liver Receptor Lig T0901317 Decreases Amyloid Beta Production in Vitro in a Mouse Model of Alzheimer';s Disease. The Journal of Biological Chemistry. 280(6): 4079-4088, 2005. [WB, mouse]
Gaus, K., et al. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. The FASEB Journal. 10.1096/fr.03-0486fje., 2004.
Denis, M., et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry. 279(40):41529-41536, 2004. [IP, human]
Sahoo, D., et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research. 45: 1122-1131, 2004. [WB, rat]
Brown III, J., et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer's Disease. The Journal of Biological Chemistry. 279(33): 34674-34681, 2004. [WB, human]
Krimbou, L., et al. Molecular interactions between apoE ABCA1: impact on apoE lipidation. Journal of Lipid Research. 45:839-848, 2004. [IP, human]
Field, F., et al. LXR/RXR lig activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research. 45:905-913, 2004. [WB, human]
Sun, Y: Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694, 2003. [WB, mouse]
Wagner, B: Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 SREBP1 Gene Expression. Molecular Cellular Biology, Aug. 2003, pp. 5780-5789. [WB, mouse]
Bortnick, A: Identification characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878, 2003. [WB, human]
Denis, M., et al. Molecular Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry. 279(9):7384-7394,2004. [IP, human]
Gaus, K., et al. Visualizing lipid structure raft domains in living cells with two- photon microscopy. PNAS. 100(26): 15554-15559, 2003. [ICC, mouse/human]
Krimbou, et al. Molecular Interactions between Apolipoprotein E the ATP-binding Cassette Transporter A1 J Biol Chem. 45: 839-848, 2004. [IP, human]
Witting, S. et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry. 278(41):40121-40127, 2003. [WB, mouse]
Sun, Y., et al. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. Journal of Biological Chemistry. 278(30):27688-37694, 2003. [WB, mouse]
Koldamova, R., et al. 22R-Hydroxycholestrol 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression Cholesterol Efflux in Brain Cells Decrease Amyloid beta Secretion. Journal of Biological Chemistry. 278(15):13244-13256, 2003. [WB/IHC/IF, rat]
Fukumoto, H., et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry. 277(50):48508-48513, 2002. [WB, human/mouse]
Feng, B., et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry. 277(45):43271-73280, 2002. [WB, mouse]
Escher, G., et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry. 278(13):11015-11019, 2003. [WB, human/hamster]
Wang, N., et al. A PEST sequence in ABCA1 regulates degradation by calpain protease stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation.111(1): 99-107, 2003. [IP/WB, human]
Khovidhunkit, W., et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: differential role of LXR. Journal of Lipid Research. 44: 1728-1736, 2003. [WB, mouse]
Le Lay, S., et al. Regulation of ABCA1 expression cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res. 44(8): p. 1499-1507, 2003. [WB, mouse]
Basso, F., et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol plasma HDL cholestrol concentrations. Journal of Lipid Research. 44:296-302, 2003. [WB, mouse]
Terasaka, N., et al. T-0901317, a synthetic liver X receptor lig, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters. 536: 6-11, 2003. [IHC-P, mouse]
Haidar, B., et al. camp induces ABC1 phosphorylation activity promotes cholesterol efflux from fibroblasts. Journal of Lipid Research. 43(12):2087-2094,2002. [IP, human]
Burgess, J.W., et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press. 277(35): 31318-31326, 2002. [WB, human/mouse]
Murthy, S., et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J. Lipid Research. 43: 1054-1064, 2002. [WB, human]