ABCA1 Antibody (NB400-105B)
ABCA1 Antibody [Biotin]
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anti-ATP binding Cassette Transporter 1 antibody, anti-ATP-binding cassette sub-family A (ABC1) member 1 antibody, anti-OTTHUMP00000021833 antibody, anti-MGC165011 antibody, anti-MGC164864 antibody, anti-Membrane bound antibody, anti-FLJ14958 antibody, anti-ATP binding cassette sub-family A member 1 antibody, anti-ATP binding cassette sub family A ABC1 member 1 antibody, anti-ATP binding cassette sub family A member 1 antibody, anti-ATP binding Cassette 1 antibody, anti-ABCA 1 antibody, anti-TD antibody, anti-ABC 1 antibody, anti-TGD antibody, anti-HDLDT1 antibody, anti-Cholesterol Efflux Regulatory Protein antibody, anti-CERP antibody, anti-ATP-binding Cassette Transporter 1 antibody, anti-ATP-binding Cassette 1 antibody, anti-ABC-1 antibody, anti-ABC1 antibody, anti-ABC Transporter 1 antibody, |
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ABCA1 Antibody [Biotin] Summary: | | Gene: | ABCA1 |
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| Purity: | Affinity purified |
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| Host: | Rabbit |
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| | Specificity: | This is specific for ABCA1. |
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| Publications: | Antibody has been mentioned in at least 23 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional ABCA1 Products ABCA1 Antibody [Biotin] Details: | Conjugate: | Biotin |
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| Immunogen: | Partial peptide sequence (peptide used resides somewhere between a.a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information. |
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| Localization: | Astrocyte Marker |
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| Marker: | Astrocyte Marker |
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NB 400-105 reacts with human, rat, and mouse ABCA1. Other species have not been tested. |
| Uses: | NB 400-105 may be used for Western analysis where a band representing ABCA1 is seen at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Other applications have been reported in literature using this antibody including immunofluorescence, immunohistochemistry, flow cytometry, and immunocytochemistry. |
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| Dilutions: | Flow Cytometry ,
immunofluorescence ,
immunoprecipitation ,
Western Blot 1:500,
Immunocytochemistry ,
Immunohistochemistry-Paraffin 1:200 |
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| Unit Size: | 0.1 ml |
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| Concentration: | 1.0 mg/ml |
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| Conjugated Antibodies: | 4 Conjugated Antibodies |
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Packaging: | Storage: | Store at 4 °C. Do not freeze. |
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| Buffer: | PBS |
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| Preservative: | 0.02% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
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Images (3) Reviews (0) Related Products (25) Exploring ABCA1 Working with ABCA1
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Jump to: Publications, Images, Ask a Question
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Working with ABCA1
ABCA1 Antibody [Biotin] Images (3) Western Blot: ABCA1 Antibody [NB400-105B] - Western Blot analysis of ABCA1, using NB400-105. Samples: 40 ug of total cell post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol. |  Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate |  Detection of ABCA1 in prostate epithelium showing luminal and membrane staining using NB400-105. |
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Publications (23)ABCA1 Antibody [Biotin] Product Specific:- Brown III, J., et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer
- Krimbou, L., et al. Molecular interactions between apoE ABCA1: impact on apoE lipidation. Journal of Lipid Research. 45:839-848, 2004. [IP, human]
more... - Field, F., et al. LXR/RXR lig activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research. 45:905-913, 2004. [WB, human]
- Sun, Y: Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694, 2003. [WB, mouse]
- Wagner, B: Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 SREBP1 Gene Expression. Molecular Cellular Biology, Aug. 2003, pp. 5780-5789. [WB, mouse]
- Bortnick, A: Identification characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878, 2003. [WB, human]
- Denis, M., et al. Molecular Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry. 279(9):7384-7394,2004. [IP, human]
- Gaus, K., et al. Visualizing lipid structure raft domains in living cells with two- photon microscopy. PNAS. 100(26): 15554-15559, 2003. [ICC, mouse/human]
- Krimbou, et al. Molecular Interactions between Apolipoprotein E the ATP-binding Cassette Transporter A1 J Biol Chem. 45: 839-848, 2004. [IP, human]
- Witting, S. et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry. 278(41):40121-40127, 2003. [WB, mouse]
- Sun, Y., et al. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. Journal of Biological Chemistry. 278(30):27688-37694, 2003. [WB, mouse]
- Koldamova, R., et al. 22R-Hydroxycholestrol 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression Cholesterol Efflux in Brain Cells Decrease Amyloid beta Secretion. Journal of Biological Chemistry. 278(15):13244-13256, 2003. [WB/IHC/IF, rat]
- Fukumoto, H., et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry. 277(50):48508-48513, 2002. [WB, human/mouse]
- Feng, B., et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry. 277(45):43271-73280, 2002. [WB, mouse]
- Escher, G., et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry. 278(13):11015-11019, 2003. [WB, human/hamster]
- Wang, N., et al. A PEST sequence in ABCA1 regulates degradation by calpain protease stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation.111(1): 99-107, 2003. [IP/WB, human]
- Khovidhunkit, W., et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: differential role of LXR. Journal of Lipid Research. 44: 1728-1736, 2003. [WB, mouse]
- Le Lay, S., et al. Regulation of ABCA1 expression cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res. 44(8): p. 1499-1507, 2003. [WB, mouse]
- Basso, F., et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol plasma HDL cholestrol concentrations. Journal of Lipid Research. 44:296-302, 2003. [WB, mouse]
- Terasaka, N., et al. T-0901317, a synthetic liver X receptor lig, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters. 536: 6-11, 2003. [IHC-P, mouse]
- Haidar, B., et al. camp induces ABC1 phosphorylation activity promotes cholesterol efflux from fibroblasts. Journal of Lipid Research. 43(12):2087-2094,2002. [IP, human]
- Burgess, J.W., et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press. 277(35): 31318-31326, 2002. [WB, human/mouse]
- Murthy, S., et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J. Lipid Research. 43: 1054-1064, 2002. [WB, human]
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Jump to: Conjugated Antibodies, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsConjugated Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ABCA1 Antibody [DyLight 488] | NB400-105G | Hu, Mu, Rt | FACS, ICC, IF, IHC-P, IP, WB | | (92) | | | | ABCA1 Antibody [DyLight 550] | NB400-105R | Hu, Mu, Rt | FACS, ICC, IF, IHC-P, IP, WB | | (92) | | | | ABCA1 Antibody [DyLight 650] | NB400-105C | Hu, Mu, Rt | FACS, ICC, IF, IHC-P, IP, WB | | (92) | | | | ABCA1 Antibody [HRP] | NB400-105H | Hu, Mu, Rt | FACS, ICC, IF, IHC-P, IP, WB | (3) | (23) | | |
Peptides and ProteinsPrimary Antibodies (22)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| ABCA1 Antibody | NB400-105 | Ha, Hu, Mu, Rt | FACS, ICC, IF, IHC-P, IP, WB | (4) | (113) | | | | ABCA1 Antibody (HJ1) - Astrocyte Marker | NB100-2068 | Hu, Mu, Rt | IHC-P, WB | (2) | (4) | | | | ABCA1 Antibody (3A1.891.3) - Astrocyte Marker | NB400-164 | Hu(-), Mu | WB | (1) | (2) | | | | ABCA1 Antibody (5A1-1422.11) | NB400-165 | Mu | FACS | (1) | (2) | | | | ABCA1 Antibody (A00121.01) - Astrocyte Marker | NB100-1663 | Ch, Hu, Mu | IHC-P, PEP-ELISA, WB | (2) | (3) | | | | ... and 17 more. See All » |
RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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