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ABCA1 Antibody (NB400-105) (0.1 ml)

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	Catalog Number	NB400-105 (Sample Size Available)
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(see alternate names)

anti-MGC164864 antibody, anti-EC 3.6.3.41 antibody, anti-FLJ14958 antibody, anti-EC 3.6.3 antibody, anti-HDLDT1 antibody, anti-EC 2.8.1.8 antibody, anti-ATP-binding cassette transporter 1 antibody, anti-EC 2.7.7.8 antibody, anti-CERP antibody, anti-ATP-binding cassette 1 antibody, anti-ABC-1 antibody, anti-membrane-bound antibody, anti-Cholesterol efflux regulatory protein antibody, anti-ABC1ATP-binding cassette transporter A1 antibody, anti-TGDATP-binding cassette sub-family A member 1 antibody, anti-MGC165011 antibody, anti-ATP-binding cassette, sub-family A (ABC1), member 1 antibody

ABCA1 Antibody Summary:

Species:	Hu, Mu, Rt, Ha	Gene:	ABCA1
Applications:	WB, FACS, ICC, IF, IHC-P, IP	Purity:	Affinity purified
Clonality:	Polyclonal	Host:	Rabbit
Specificity:	This antibody is specific for ABCA1.
Publications:	NB400-105 has been mentioned in at least 129 Publications

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ABCA1 Antibody Details:

Immunogen:	Partial peptide sequence (peptide used resides somewhere between a.a 1100-1300) of the human ABCA1 gene. Actual immunogen sequence is proprietary information.
Localization:	Astrocyte Marker
Marker:	Astrocyte Marker

Species Reactivity:

NB 400-105 reacts with human, hamster, rat and mouse ABCA1. Other species have not been tested.

Applications:

Uses:	NB400-105 may be used for Western blot, histochemical applications, ICC/IF and Flow Cytometry. For western analysis a band representing ABCA1 is seen at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Other applications have been reported in literature using this antibody including immunofluorescence, immunohistochemistry, flow cytometry, and immunocytochemistry. NOTE: It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel.
Dilutions:	Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry-Paraffin 1:200, Immunoprecipitation, Western Blot 1:500
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	1.0 mg/ml
Positive Controls:	1 Positive Controls
Conjugated Antibodies:	5 Conjugated Antibodies

Packaging:

Storage:	Store at 4 °C. Do not freeze.
Buffer:	Tris-citrate pH 7-8
Preservative:	0.02% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: ABCA1

Entrez:	19 (Human)
	11303 (Mouse)
	313210 (Rat)
Uniprot:	O95477 (Human)

Related Genes:

Background:

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

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Reviews (2)

Anonymous on 9/27/2011

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ABCA1 Antibody Images (5)

	Western Blot: ABCA1 Antibody [NB400-105] - Western Blot analysis of ABCA1, using NB400-105. Samples: 40 ug of total cell post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol.

	Western Blot: ABCA1 antibody [NB400-105] - Detection of ABCA1 in mouse peritoneal macrophages using NB 400-105 (Lot L). ECL exposure, 1 min. Lane 4: T09 uninduced lysate Lane 5: T09 induced lysate

	Western Blot: ABCA1 Antibody [NB400-105] - Western blot analysis of RAW 264.7 Whole cell Lysate Kit [NBP1-30159] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2).

	Immunohistochemistry: ABCA1 antibody [NB400-105] - Detection of ABCA1 in prostate epithelium showing luminal and membrane staining using NB400-105.

	Immunocytochemistry: ABCA1 Antibody [NB400-105] - ABCA1 antibody was tested at 1:100 in HeLa cells with FITC (green). alpha-Tubulin antibody NB100-690 was tested at a 1:500 dilution using Mouse IgG secondary Antibody NB710-94914 at 1:1000 [HiLyte Fluor 555] (red). Nuclei were counterstained with Dapi (blue).

Reviews (2) Have you used this product? Review this product to earn Novus Rewards.

Reviewed by:
Anonymous 9/27/2011

Application:	Western Blot
Sample Tested:	Mouse
Species:	Mouse

Reviewed by:
Anonymous 6/15/2009

Application:	Western Blot
Sample Tested:	Rat astrocyte, Human Astrocyte, and Raw Mouse Neuron
Sample Loaded:	25ug to 50ug
Species:	Other
Species Other:	Rat, Human, and Mouse

Blocking Buffer:	5% Milk w/ TBST
Blocking Time:	1 hour
Blocking Temperature:	room temperature

Dilution Ratio:	1/1000
Incubation Time:	3 hours
Incubation Temperature:	room temperature
Incubation Dilution Buffer:	1:1000

Description:	anti-Rabbit IgG HRP
Dilution Ratio:	1/1000

Exposure Time:	1 hour
Positive Control:	HC/RA
Did you observe bands?:	Yes
Specific Bands:	200 kDa

Publications (129)

ABCA1 Antibody Product Specific:

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Fournier N, Attia N, Rousseau-Ralliard D, et al. Deleterious impact of elaidic trans fatty acid on ABCA1-mediated cholesterol efflux from mouse and human macrophages. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 2011 Oct 12. [PMID:
Boadu E, Nelson R, Francis GA. ABCA1-dependent mobilization of lysosomal cholesterol requires functional Niemann-Pick C2 but not Niemann-Pick C1 protein. Biochimica Et Biophysica Acta. 2011 Dec 10. [PMID: 22179027]
Zhang J, Kris-Etherton PM, Thompson JT, et al. Alpha-linolenic acid increases cholesterol efflux in macrophage-derived foam cells by decreasing stearoyl CoA desaturase 1 expression: evidence for a farnesoid-X-receptor mechanism of action. J Nutr Biochem. 2011 Jun 7. [PMID: 21658928]
Ting TC, Miyazaki-Anzai S, Masuda M, et al. Increased lipogenesis and stearate accelerate vascular calcification in calcifying vascular cells. J. Biol. Chem. 2011 Jul 8;286(27):23938-23949. [PMID: 21596756]
Storey SM, McIntosh AL, Huang H, et al. Intracellular Cholesterol Binding Proteins Enhance HDL-mediated Cholesterol Uptake in Cultured Primary Mouse Hepatocytes. American Journal of Physiology. Gastrointestinal and Liver Physiology. 2012 Jan 12. [PMID: 22241858]
McIntosh AL, Atshaves BP, Storey SM, et al. Loss of liver fatty acid binding protein significantly alters hepatocyte plasma membrane microdomains. Journal of Lipid Research. 2012 Jan 5. [PMID: 22223861]
Hossain MA, Ngeth S, Chan T, et al. Lipid-bound apolipoproteins in tyrosyl radical-oxidized HDL stabilize ABCA1 like lipid-free apolipoprotein A-I. BMC Biochemistry. 2012 Jan 1613(1):1. . [PMID: 22248050]
Cui HL, Grant A, Mukhamedova N, et al. HIV-1 Nef mobilizes lipid rafts in macrophages through a pathway that competes with ABCA1-dependent cholesterol efflux. Journal of Lipid Research. 2012 Jan 19. [PMID: 22262807]
Coleman EM, Walker TN, Hildreth JEK. Loss of Niemann Pick type C proteins 1 and 2 greatly enhances HIV infectivity and is associated with accumulation of HIV Gag and cholesterol in late endosomes/lysosomes. Virology Journal. 2012 Jan 249(1):31. . [PMID: 22273177]
Kammerer I, Ringseis R, Biemann R, et al. 13-hydroxy linoleic acid increases expression of the cholesterol transporters ABCA1, ABCG1 and SR-BI and stimulates apoA-I-dependent cholesterol efflux in RAW264.7 macrophages. Lipids in Health and Disease. 2011 Nov 3010(1):222. [PMID: 22129452]
Le Lay S, Rodriguez M, Jessup W, et al. Caveolin-1-mediated apolipoprotein a-I membrane binding sites are not required for cholesterol efflux. PLoS ONE. 2011.[PMID: 21858084]
Oligomeric ABCA1 and ApoA-I-Mediated Lipid Removal: Molecular Evidence that ApoA-I Interaction with ABCA1 Activatesthe Phosphatidylcholine Biosynthesis Pathway. J Lipid Res. 2011 Aug 16. [PMID: 21846716]
Wong BXW, Kyle RA, Croft KD, et al. Modulation of Macrophage Fatty Acid Content and Composition by Exposure to Dyslipidemic Serum in Vitro. Lipids. 2011 Apr;46(4):371-80.[PMID 21286835]
Hong C, Walczak R, Dhamko H, et al. Constitutive activation of LXR in macrophages regulates metabolic and inflammatory gene expression: identification of ARL7 as a direct target. J. Lipid Res. 2011 Mar;52(3):531-539.[PMID 21187453]
Hauff KD, Mitchell RW, Xu FY, et al. Mifepristone Treatment Results in Differential Regulation of Glycerolipid Biosynthesis in Baby Hamster Kidney Cells Expressing a Mifepristone-Inducible ABCA1.Lipids. 2011 Sep;46(9):795-804.[PMID 21710238]
Lee JY, Karwatsky J, Ma L, Zha X. ABCA1 increases extracellular ATP to mediate cholesterol efflux to apoA-I. Am J Physiol Cell Physiol. 2011 Oct;301(4):C886-94. [PMID 21697542]
Nikitina L, Wenger F, Baumann M, et al. Expression and localization pattern of ABCA1 in diverse human placental primary cells and tissues. Placenta. 2011 Jun;32(6):420-30.[PMID 21501868]
Terwel D, Steffensen KR, Verghese PB, et al. Critical Role of Astroglial Apolipoprotein E and Liver X Receptor-{alpha} Expression for Microglial A{beta} Phagocytosis.J Neurosci. 2011 May 11;31(19):7049-59. [PMID 21562267]
Chen J, Costa LG, Guizzetti M. Retinoic acid isomers up-regulate ATP binding cassette a1 and g1 and cholesterol efflux in rat astrocytes: implications for their therapeutic and teratogenic effects. J Pharmacol Exp Ther. 2011 Sep;338(3):870-8. [PMID 21628419]
Loane DJ, Washington PM, Vardanian L, et al. Modulation of ABCA1 by an LXR Agonist Reduces Beta-Amyloid Levels and Improves Outcome after Traumatic Brain Injury. J. Neurotrauma. 2011 Feb. [PMID: 21175399]
Elali A, Hermann DM. Liver X receptor activation enhances blood-brain barrier integrity in the ischemic brain and increases the abundance of ATP-binding cassette transporters ABCB1 and ABCC1 on brain capillary cells. Brain Pathol. 2011 Jul 18.[PMID 21767321]
Xue JH, Yuan Z, Wu Y, et al. High glucose promotes intracellular lipid accumulation in vascular smooth muscle cells by impairing cholesterol influx and efflux balance. Cardiovasc Res. 2010 Apr 1;86(1):141-50. [PMID: 20007688](Human, WB)
J. E. McLaren, C. J. Calder, B. P. McSharry, et al. The TNF-Like Protein 1A-Death Receptor 3 Pathway Promotes Macrophage Foam Cell Formation In Vitro. J. Immunol. 2010; 184 (10): 5827-5834. [PMID: 20410491]
Zhang Y, Yin L, Anderson J, Ma H, et al. Identification of novel pathways that control farnesoid X receptor-mediated hypocholesterolemia. J Biol Chem. 2010 Jan 29;285(5):3035-43. [PMID: 19996107] (Mouse, Rat, WB)
Sporstøl M, Mousavi SA, Eskild W, et al. ABCA1, ABCG1 and SR-BI: hormonal regulation in primary rat hepatocytes and human cell lines. BMC Mol Biol. 2007 Jan 22;8:5.[PMID: 17241464]
Crisafulli C, Di Paola R, Mazzon E, et al. Liver X receptor agonist treatment reduced splanchnic ischemia and reperfusion injury. J Leukoc Biol. 2009 Dec 22. [PMID: 20028773]
Maitra U, Parks JS, Li L. An innate immunity signaling process suppresses macrophage ABCA1 expression through IRAK-1-mediated downregulation of retinoic acid receptor alpha and NFATc2. Mol Cell Biol. 2009 Nov;29(22):5989-97. [PMID: 19752193]
Tang C, Liu Y, Kessler PS, et al. The macrophage cholesterol exporter ABCA1 functions as an anti-inflammatory receptor. J Biol Chem. 2009 Nov 20;284(47):32336-43. [PMID: 19783654]
Sirek AS, Liu L, Naples M, et al. Insulin Stimulates the Expression of Carbohydrate Response Element Binding Protein (ChREBP) by Attenuating the Repressive Effect of Pit-1, Oct-1/Oct-2, Unc-86 Homeodomain Protein Octamer Transcription Factor-1. Endocrinology 2009;150(8):3483-3492. [pubmed: 19359385]
Ronis MJ, Chen Y, Badeaux J, Badger TM. Dietary soy protein isolate attenuates metabolic syndrome in rats via effects on PPAR, LXR, and SREBP signaling. J Nutr.2009 Aug;139(8):1431-8. Epub 2009 Jun 10.
Duncan KG, Hosseini K, Bailey KR, et al. Expression of reverse cholesterol transport proteins ATP-binding cassette A1 (ABCA1) scavenger receptor BI (SR-BI) in the retina retinal pigment epithelium. Br J Ophthalmol 2009;93(8):1116-1120. [pubmed: 19304587]
Welch C, Santra MK, El-Assaad W, et al. Identification of a Protein, G0S2, That Lacks Bcl-2 Homology Domains Interacts with Antagonizes Bcl-2. Cancer Res 2009:0008-5472.CAN-0009-0128. [pubmed: 19706769]
Xu G, Watanabe T, Iso Y, et al. Preventive Effects of Heregulin-{beta}1 on Macrophage Foam Cell Formation Atherosclerosis. Circ Res 2009:CIRCRESAHA.109.193870. [pubmed: 19644050]
Liu J, Huan C, Chakraborty M, et al. Macrophage Sphingomyelin Synthase 2 Deficiency Decreases Atherosclerosis in Mice. Circ Res 2009;105(3):295-303. [pubmed: 19590047]
Kim HJ, Moradi H, Yuan J, et al. Renal mass reduction results in accumulation of lipids dysregulation of lipid regulatory proteins in the remnant kidney. Am J Physiol Renal Physiol 2009;296(6):F1297-1306. [pubmed: 19357177]
Bujold K, Rhainds D, Jossart C, et al. CD36-mediated cholesterol efflux is associated with PPAR{gamma} activation via a MAPK-dependent COX-2 pathway in macrophages. Cardiovasc Res 2009:cvp118. [pubmed: 19377069]
Ouvrier A, Cadet R, Vernet P, et al. LXR ABCA1 control cholesterol homeostasis in the proximal mouse epididymis in a cell specific manner. J Lipid Res 2009:M800657-JLR800200 [pubmed: 19395734]
Hans CP, Zerfaoui M, Naura AS, et al. Thieno[2,3-c]Isoquinolin-5-one, a Potent Poly(ADP-Ribose) Polymerase Inhibitor, Promotes Atherosclerotic Plaque Regression in High-Fat Diet-Fed Apolipoprotein E-Deficient Mice: Effects on Inflammatory Markers Lipid Content. J Pharmacol Exp Ther 2009;329(1):150-158 [pubmed: 19124646]
Bhatnagar S, Damron HA, Hillgartner FB. Fibroblast Growth Factor-19, a Novel Factor That Inhibits Hepatic Fatty Acid Synthesis. J Biol Chem 2009;284(15):10023-10033 [pubmed: 19233843]
Dang H, Liu Y, Pang W, et al. Suppression of 2,3-Oxidosqualene Cyclase by High Fat Diet Contributes to Liver X Receptor-{alpha}-mediated Improvement of Hepatic Lipid Profile. J Biol Chem 2009;284(10):6218-6226 [pubmed: 19119143]
Pelletier RM, Yoon SR, Akpovi CD, et al. Defects in the regulatory clearance mechanisms favor the breakdown of self-tolerance during spontaneous autoimmune orchitis. Am J Physiol Regulatory Integrative Comp Physiol 2009;296(3):R743-762 [pubmed: 19052317]
Bhatnagar S, Damron HA, Hillgartner FB. Fibroblast Growth Factor-19, a Novel Factor that Inhibits Hepatic Fatty Acid Synthesis. J Biol Chem. 2009 Apr 10;284(15):10023-33. [pubmed: 19233843]
Nakanishi S, Vikstedt R, Soderlund S, et al. Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity. J Lipid Res. 2009 Feb;50(2):183-92. [pubmed: 18787236]
Pou J, Rebollo A, Roglans N, Sánchez RM, Vázquez-Carrera M, Laguna JC, Pedro-Botet J, Alegret M. Ritonavir increases CD36, ABCA1 and CYP27 expression in THP-1 macrophages. Exp Biol Med (Maywood). 2008 Dec;233(12):1572-82. Epub 2008 Oct 10.
Cartagena CM, Ahmed F, Burns MP, Pajoohesh-Ganji A, Pak DT, Faden AI, Rebeck GW. Cortical injury increases cholesterol 24S hydroxylase (Cyp46) levels in the rat brain. J Neurotrauma. 2008 Sep;25(9):1087-98.
Robichaud JC, Francis GA, Vance DE. A role for hepatic scavenger receptor class B, type I in decreasing high density lipoprotein levels in mice that lack phosphatidylethanolamine N-methyltransferase. J Biol Chem. 2008 Dec 19;283(51):35496-506. Epub 2008 Oct 8.
Ni S, Ma L, Denis M, et al. ABCA1-mediated cholesterol efflux generates microparticles in addition to HDL through processes governed by membrane rigidity. J Lipid Res. 2009 Mar;50(3):456-66. [pubmed: 18941142]
Delvecchio CJ, Bilan P, Nair P, Capone JP. LXR-induced reverse cholesterol transport in human airway smooth muscle is mediated exclusively by ABCA1. Am J Physiol Lung Cell Mol Physiol. 2008 Nov;295(5):L949-57. Epub 2008 Sep 26.
Paul V, Meyer HH, Leidl K, Soumian S, Albrecht C. A novel enzyme immunoassay specific for ABCA1 protein quantification in human tissues and cells. J Lipid Res. 2008 Oct;49(10):2259-67. Epub 2008 Jun 9.
Peng D, Hiipakka RA, Dai Q, Guo J, Reardon CA, Getz GS, Liao S. Antiatherosclerotic effects of a novel synthetic tissue-selective steroidal liver X receptor agonist in low-density lipoprotein receptor-deficient mice. J Pharmacol Exp Ther. 2008 Nov;327(2):332-42. Epub 2008 Aug 22.
Mukhamedova N, Escher G, D'Souza W, Tchoua U, Grant A, Krozowski Z, Bukrinsky M, Sviridov D. Enhancing apolipoprotein A-I-dependent cholesterol efflux elevates cholesterol export from macrophages in vivo. J Lipid Res. 2008 Nov;49(11):2312-22. Epub 2008 Jul 12.
Tancevski I, Wehinger A, Demetz E, Eller P, Duwensee K, Huber J, Hochegger K, Schgoer W, Fievet C, Stellaard F, Rudling M, Patsch JR, Ritsch A. Reduced plasma high-density lipoprotein cholesterol in hyperthyroid mice coincides with decreased hepatic adenosine 5'-triphosphate-binding cassette transporter 1 expression. Endocrinology. 2008 Jul;149(7):3708-12. Epub 2008 Apr 3.
Kumar R, McClain D, Young R, Carlson GA. Cholesterol transporter ATP-binding cassette A1 (ABCA1) is elevated in prion disease and affects PrPC and PrPSc concentrations in cultured cells. J Gen Virol. 2008 Jun;89(Pt 6):1525-32.
Hastie C, Masters JR, Moss SE, Naaby-Hansen S. Interferon-{gamma} Reduces Cell Surface Expression of Annexin 2 Suppresses the Invasive Capacity of Prostate Cancer Cells. J Biol Chem. May 2, 2008;283(18):12595-603.
Bowden K, Ridgway ND. OSBP negatively regulates ABCA1 protein stability. J Biol Chem. 2008 Jun 27;283(26):18210-7. Epub 2008 May 1.
Frank PG, Pavlides S, Cheung MW, Daumer K, Lisanti MP. Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physiol. 2008 Jul;295(1):C242-8. Epub 2008 May 28.
Montoudis, A. et al. Intestinal fatty acid binding protein regulates mitochonfrion beta-oxidation cholesterol uptake. J. Lipid Res. January 30, 2008; Volume 49: 961-972. (WB)
Smoak K, Madenspacher J, Jeyaseelan S, Williams B, Dixon D, Poch KR, Nick JA, Worthen GS, Fessler MB. Effects of liver X receptor agonist treatment on pulmonary inflammation and host defense. J Immunol. 2008 Mar 1;180(5):3305-12.
Ouimet M, Wang M-D, Cadotte N, et al. Epoxycholesterol Impairs Cholesteryl Ester Hydrolysis in Macrophage Foam Cells, Resulting in Decreased Cholesterol Efflux. Arterioscler Thromb Vasc Biol 2008;28(6):1144-1150. (Western Blot)
Watanabe T, Nishio K, Kanome T, Matsuyama TA, Koba S, Sakai T, Sato K, Hongob S, Nose K, Ota H, Kobayashi Y, Katagiri T, Shichiri M, Miyazaki A. Impact of salusin-alpha and -beta on human macrophage foam cell formation and coronary atherosclerosis. Circulation. 2008 Feb 5;117(5):638-48. Epub 2008 Jan 22.
Henry-Berger J, Mouzat K, Baron S, Bernabeu C, Marceau G, Saru JP, Sapin V, Lobaccaro JM, Caira F. Endoglin (CD105) expression is regulated by the liver X receptor alpha (NR1H3) in human trophoblast cell line JAR. Biol Reprod. 2008 Jun;78(6):968-75. Epub 2008 Feb 14.
Zhou X, He W, Huang Z, Gotto AM Jr, Hajjar DP, Han J. Genetic deletion of low density lipoprotein receptor impairs sterol-induced mouse macrophage ABCA1 expression. A new SREBP1-dependent mechanism. J Biol Chem. 2008 Jan 25;283(4):2129-38. Epub 2007 Nov 20.
Yan D, Mäyränpää MI, Wong J, Perttilä J, Lehto M, Jauhiainen M, Kovanen PT, Ehnholm C, Brown AJ, Olkkonen VM. OSBP-related protein 8 (ORP8) suppresses ABCA1 expression and cholesterol efflux from macrophages. J Biol Chem. 2008 Jan 4;283(1):332-40. Epub 2007 Nov 8.
Jacobs RL, Lingrell S, Zhao Y, Francis GA, Vance DE. Hepatic CTP:phosphocholine cytidylyltransferase-alpha is a critical predictor of plasma high density lipoprotein and very low density lipoprotein. J Biol Chem. 2008 Jan 25;283(4):2147-55. Epub 2007 Nov 27.
Hastie C, Masters JR, Moss SE, Naaby-Hansen S. Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells. J Biol Chem. 2008 May 2;283(18):12595-603. Epub 2008 Jan 22.
Zhu M, Fu Y, Hou Y, et al. Laminar Shear Stress Regulates Liver X Receptor in Vascular Endothelial Cells. Arterioscler Thromb Vasc Biol. March 1, 2008;28(3):527-33. (Western Blot)
Wang N, Yvan-Charvet L, Lütjohann D, Mulder M, Vanmierlo T, Kim TW, Tall AR. ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain. FASEB J. 2008 Apr;22(4):1073-82. Epub 2007 Nov 26.
Out R, Jessup W, Le Goff W, Hoekstra M, Gelissen IC, Zhao Y, Kritharides L, Chimini G, Kuiper J, Chapman MJ, Huby T, Van Berkel TJ, Van Eck M. Coexistence of foam cells and hypocholesterolemia in mice lacking the ABC transporters A1 and G1. Circ Res. 2008 Jan 4;102(1):113-20. Epub 2007 Oct 25.
Babaev VR, Ishiguro H, Ding L, Yancey PG, Dove DE, Kovacs WJ, Semenkovich CF, Fazio S, Linton MF. Macrophage expression of peroxisome proliferator-activated receptor-alpha reduces atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation. 2007 Sep 18;116(12):1404-12. Epub 2007 Aug 27.
Bates SR, Tao JQ, Yu KJ, Borok Z, Crandall ED, Collins HL, Rothblat GH. Expression and biological activity of ABCA1 in alveolar epithelial cells. Am J Respir Cell Mol Biol. 2008 Mar;38(3):283-92. Epub 2007 Sep 20.
Talukdar S, Bhatnagar S, Dridi S, Hillgartner FB. Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-alpha gene transcription by the liver X receptor agonist T0-901317. J Lipid Res. 2007 Dec;48(12):2647-63. Epub 2007 Sep 6.
Hoe HS, Cooper MJ, Burns MP, Lewis PA, van der Brug M, Chakraborty G, Cartagena CM, Pak DT, Cookson MR, Rebeck GW. The metalloprotease inhibitor TIMP-3 regulates amyloid precursor protein and apolipoprotein E receptor proteolysis. J Neurosci. 2007 Oct 3;27(40):10895-905.
Hassan HH, Denis M, Lee DY, Iatan I, Nyholt D, Ruel I, Krimbou L, Genest J. Identification of an ABCA1-dependent phospholipid-rich plasma membrane apolipoprotein A-I binding site for nascent HDL formation: implications for current models of HDL biogenesis. J Lipid Res. 2007 Nov;48(11):2428-42. Epub 2007 Jul 26.
During A, Harrison EH. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. J Lipid Res. 2007 Oct;48(10):2283-94. Epub 2007 Jul 20.
Atshaves BP, McIntosh AL, Payne HR, Gallegos AM, Landrock K, Maeda N, Kier AB, Schroeder F. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. J Lipid Res. 2007 Oct;48(10):2193-211. Epub 2007 Jul 3.
Gaus K, Gratton E, Kable EP, Jones AS, Gelissen I, Kritharides L, Jessup W. Visualizing lipid structure and raft domains in living cells with two-photon microscopy. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15554-9. Epub 2003 Dec 12.
Gerbod-Giannone, M-C. et al. TNFa induces ABCA1 through NF-kappaB in macrophage in phagocytes ingesting apoptotic cells. PNAS. 103:3112-3117, 2006.
Trogan, E., et al. Gene expression changes in foam cells the role of chemokine receptor CCR7 during athersclerosis regerssion in Apo-E-deficient mice. PNAS. 103:3781-3786, 2006.
Reddy ST, Hama S, Ng C, Grijalva V, Navab M, Fogelman AM. ATP-binding cassette transporter 1 participates in LDL oxidation by artery wall cells. Arterioscler Thromb Vasc Biol. 2002 Nov 1;22(11):1877-83.
Forcheron F, Legedz L, Chinetti G, Feugier P, Letexier D, Bricca G, Beylot M. Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression. Arterioscler Thromb Vasc Biol. 2005 Aug;25(8):1711-7. Epub 2005 Jun 16.
Boisvert WA, Rose DM, Boullier A, Quehenberger O, Sydlaske A, Johnson KA, Curtiss LK, Terkeltaub R. Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size. Arterioscler Thromb Vasc Biol. 2006 Mar;26(3):563-9. Epub 2006 Jan 12.
Duong PT, Collins HL, Nickel M, Lund-Katz S, Rothblat GH, Phillips MC. Characterization of nascent HDL particles and microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res. 2006 Apr;47(4):832-43. Epub 2006 Jan 17.
Dong, J., et al. Adenovirus-mediated overexpression of sphingomyelin synthases 1 2 increases the atherogenic potenial in mice. J. Lipid Res. 47:1307-1314, 2006.
Zhang, C., et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J. Lipid Res. 2006.
Berfield, A.K., et al. IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride. Am. J. Physiol. Renal Physiol. 290:138-147, 2006.
Zager RA, Johnson AC, Hanson SY. Proximal tubular cholesterol loading after mitochondrial, but not glycolytic, blockade. Am J Physiol Renal Physiol. 2003 Dec;285(6):F1092-9. Epub 2003 Sep 2.
Dong J, Liu J, Lou B, Li Z, Ye X, Wu M, Jiang XC. Adenovirus-mediated overexpression of sphingomyelin synthases 1 and 2 increases the atherogenic potential in mice. J Lipid Res. 2006 Jun;47(6):1307-14. Epub 2006 Feb 28.
Khovidhunkit, W. et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research. 44: 1728-1736, 2003. [WB, mouse]
Su, Y., et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice Up-regulates ABC Transporters. Molecular Therapy. 0:1-8,2003. [WB, mouse]
Glaros, E.N., et al. Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-1 pathway. J. Biol. Chem. 280(26): 24515-24523, 2005. [WB, human]
Zeng, L., et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J. Biol. Chem. 279: 48801-48807, 2004. [WB, human]
O'Connell BJ, Denis M, Genest J. Cellular physiology of cholesterol efflux in vascular endothelial cells. Circulation. 2004 Nov 2;110(18):2881-8. Epub 2004 Oct 18.
Agassandian M, Mathur SN, Zhou J, Field FJ, Mallampalli RK. Oxysterols trigger ABCA1-mediated basolateral surfactant efflux. Am J Respir Cell Mol Biol. 2004 Aug;31(2):227-33. Epub 2004 Mar 23.
Dove DE, Su YR, Zhang W, Jerome WG, Swift LL, Linton MF, Fazio S. ACAT1 deficiency disrupts cholesterol efflux and alters cellular morphology in macrophages. Arterioscler Thromb Vasc Biol. 2005 Jan;25(1):128-34. Epub 2004 Oct 21.
Bared SM, Buechler C, Boettcher A, Dayoub R, Sigruener A, Grandl M, Rudolph C, Dada A, Schmitz G. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol Biol Cell. 2004 Dec;15(12):5399-407. Epub 2004 Oct 6.
Haidar, B., et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J. Biol. Chem. 279: 9963-9969, 2004. [IP, human]
Li. A., et al. Differential inhibition of macrophage foam-cell formation atherosclerosis in mice by PPAR alpha alpha, B/delta, gamma. J. Clin. Invest. 114: 1564-1576, 2004.
Ando H, Tsuruoka S, Yamamoto H, Takamura T, Kaneko S, Fujimura A. Effects of pravastatin on the expression of ATP-binding cassette transporter A1. J Pharmacol. Exp Ther. 2004 Oct;311(1):420-5. Epub 2004 Jun 21.
Koldamova, R., et al. The Liver Receptor Lig T0901317 Decreases Amyloid Beta Production in Vitro in a Mouse Model of Alzheimer';s Disease. The Journal of Biological Chemistry. 280(6): 4079-4088, 2005. [WB, mouse]
Gaus K, Kritharides L, Schmitz G, Boettcher A, Drobnik W, Langmann T, Quinn CM, Death A, Dean RT, Jessup W. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. FASEB J. 2004 Mar;18(3):574-6. Epub 2004 Jan 20.
Denis, M., et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry. 279(40):41529-41536, 2004. [IP, human]
Sahoo, D., et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research. 45: 1122-1131, 2004. [WB, rat]
Brown III, J., et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer's Disease. The Journal of Biological Chemistry. 279(33): 34674-34681, 2004. [WB, human]
Krimbou, L., et al. Molecular interactions between apoE ABCA1: impact on apoE lipidation. Journal of Lipid Research. 45:839-848, 2004. [IP, human]
Field, F., et al. LXR/RXR lig activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research. 45:905-913, 2004. [WB, human]
Sun, Y: Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694, 2003. [WB, mouse]
Wagner, B: Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 SREBP1 Gene Expression. Molecular Cellular Biology, Aug. 2003, pp. 5780-5789. [WB, mouse]
Bortnick, A: Identification characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878, 2003. [WB, human]
Denis, M., et al. Molecular Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry. 279(9):7384-7394,2004. [IP, human]
Gaus, K., et al. Visualizing lipid structure raft domains in living cells with two- photon microscopy. PNAS. 100(26): 15554-15559, 2003. [ICC, mouse/human]
Krimbou, et al. Molecular Interactions between Apolipoprotein E the ATP-binding Cassette Transporter A1 J Biol Chem. 45: 839-848, 2004. [IP, human]
Witting, S. et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry. 278(41):40121-40127, 2003. [WB, mouse]
Sun, Y., et al. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. Journal of Biological Chemistry. 278(30):27688-37694, 2003. [WB, mouse]
Koldamova, R., et al. 22R-Hydroxycholestrol 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression Cholesterol Efflux in Brain Cells Decrease Amyloid beta Secretion. Journal of Biological Chemistry. 278(15):13244-13256, 2003. [WB/IHC/IF, rat]
Fukumoto, H., et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry. 277(50):48508-48513, 2002. [WB, human/mouse]
Feng, B., et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry. 277(45):43271-73280, 2002. [WB, mouse]
Escher, G., et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry. 278(13):11015-11019, 2003. [WB, human/hamster]
Wang, N., et al. A PEST sequence in ABCA1 regulates degradation by calpain protease stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation.111(1): 99-107, 2003. [IP/WB, human]
Khovidhunkit, W., et al. Endotoxin down-regulates ABCG5 ABCG8 in mouse liver ABCA1 ABCG1 in J774 murine macrophages: differential role of LXR. Journal of Lipid Research. 44: 1728-1736, 2003. [WB, mouse]
Le Lay, S., et al. Regulation of ABCA1 expression cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res. 44(8): p. 1499-1507, 2003. [WB, mouse]
Basso, F., et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol plasma HDL cholestrol concentrations. Journal of Lipid Research. 44:296-302, 2003. [WB, mouse]
Terasaka, N., et al. T-0901317, a synthetic liver X receptor lig, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters. 536: 6-11, 2003. [IHC-P, mouse]
Haidar, B., et al. camp induces ABC1 phosphorylation activity promotes cholesterol efflux from fibroblasts. Journal of Lipid Research. 43(12):2087-2094,2002. [IP, human]
Burgess, J.W., et al. Trypsin-sensitive lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press. 277(35): 31318-31326, 2002. [WB, human/mouse]
Murthy, S., et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J. Lipid Research. 43: 1054-1064, 2002. [WB, human]

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Protocol specific for ABCA1 Antibody (NB400-105)

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Jump to: Conjugated Antibodies, Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

Related Products

Conjugated Antibodies

Name	Catalog#	Reactivity	Applications	Images	Publications
ABCA1 Antibody [DyLight 488]	NB400-105G	Hu, Mu, Rt	FACS, ICC, IF, IHC-P, IP, WB		(92)
ABCA1 Antibody [DyLight 550]	NB400-105R	Hu, Mu, Rt	FACS, ICC, IF, IHC-P, IP, WB		(92)
ABCA1 Antibody [DyLight 650]	NB400-105C	Hu, Mu, Rt	FACS, ICC, IF, IHC-P, IP, WB		(92)
ABCA1 Antibody [Biotin]	NB400-105B	Hu, Mu, Rt	FACS, ICC, IF, IHC-P, IP, WB	(3)	(23)
ABCA1 Antibody [HRP]	NB400-105H	Hu, Mu, Rt	FACS, ICC, IF, IHC-P, IP, WB	(3)	(23)

Positive Controls

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Raw 264.7 Whole Cell - T0901317 treated Kit 2 vials	NBP1-30159		WB	(1)

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
ABCA1 Peptide	NB400-105PEP				(1)
ABCA1 Partial Recombinant Protein	H00000019-Q01	Hu	ELISA, WB	(1)

Primary Antibodies (17)

Name	Catalog#	Reactivity	Applications	Images	Publications
ABCA1 Antibody (HJ1) - Astrocyte Marker	NB100-2068	Hu, Mu, Rt	IHC-P, WB	(2)	(5)
ABCA1 Antibody (3A1.891.3) - Astrocyte Marker	NB400-164	Hu(-), Mu	WB	(2)	(2)
ABCA1 Antibody (5A1-1422.11)	NB400-165	Mu	FACS	(1)	(2)
ABCA1 Antibody (A00121.01) - Astrocyte Marker	NB100-1663	Ch, Hu, Mu	IHC-P, PEP-ELISA, WB	(2)	(5)
ABCA1 Antibody	NBP1-69012	Hu, Mu	WB	(1)	(1)
... and 12 more. See All »

RNAi (1)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
ABCA1 RNAi	H00000019-R02	Hu		(1)	(4)

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Images	Publications
Rabbit IgG Antibody [HRP]	NB730-H	Rb	ELISA, ICC, IHC-P, WB		(6)
Rabbit IgG Antibody [FITC]	NB730-F	Rb	ICC, IHC-P		(1)
Rabbit IgG Antibody [Cy3]	NB120-6939	Rb	ELISA, FACS, ICC, IF, IHC-Fr, IHC-P		(2)
Rabbit IgG Antibody [Biotin]	NB730-B	Rb	ELISA, ICC, IHC-P, WB
Rabbit IgG, H/L Chains Antibody [DyLight 488]	NBP1-72944	Rb	FLISA, IF, WB	(3)
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ABCA1 in Context

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5/20/2010 MicroRNA And Host Gene Play Key Role ...