ABCA1 Antibody (5A1-1422.11) Summary:

Species:	Mu
Applications:	FACS
Clonality:	Monoclonal

Gene:	ABCA1
Purity:	Ascites
Host:	Rat

Specificity:	This is specific for ABCA1.
Publications:	Antibody has at least 2 Background References.

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

View Additional ABCA1 Products

Peptides and Proteins	Primary Antibodies
RNAi

» View all ABCA1 Related Products

ABCA1 Antibody (5A1-1422.11) Details:

Clone:	5A1-1422.11
Isotype:	IgG2a Kappa
Immunogen:	Mouse ABCA1-expressing HeLa stable transfectants.
Localization:	Cell Membrane
Marker:	Astrocyte Marker
Epitope:	Extracellular determinants on ABCA1 of an unknown position.

Species Reactivity:

Mouse.

Applications:

Uses:	NB 400-165 may be used for Flow Cytometry. NB 400-165 is not reactive in Western Blot or ELISA.
Dilutions:	Flow Cytometry 1:1,000 to 1:30,000,
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	This product is unpurified. Concentration is not relevant.
Positive Controls:	1 Positive Controls

Packaging:

Storage:	Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles.
Preservative:	0.1% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: ABCA1

Entrez	19 (Human)
	11303 (Mouse)

Background:

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

Images (1)

See all images...

Reviews (0)

Be the first to review this product for a reward!

Working with ABCA1

ABCA1 Antibody (5A1-1422.11) Images (1)

FACS analysis of Raw (red) and Raw-cAMP (blue) cells using NB 400-165 at a dilution of 1:4,000

Reviews (0) Have you used this product? Review this product to earn Novus Rewards.

Publications (2)

ABCA1 Antibody (5A1-1422.11) Background References:

Oram, JF Heinecke, JW. Physiol Rev. 85:1343-72 (2005)
Trompier, D Chimini, G. ABCA1, Alliance for Cellular Signaling Nature Publishing Group, Molecular pages. doi: 10.1038/mp.a002593.01 [website: http://www.signaling-gateway.org/molecule/](2005)

Protocols

All General Protocols

Ask a Scientist

Can't find an answer to your question about ABCA1? Ask us by using live chat or by submitting your questions below:

Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

Related Products

Positive Controls

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Raw 264.7 Whole Cell - T0901317 treated Kit 2 vials	NBP1-30159		WB	(1)

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
ABCA1 Peptide	NB400-105PEP				(1)
ABCA1 Partial Recombinant Protein	H00000019-Q01	Hu	ELISA, WB	(1)

Primary Antibodies (22)

Name	Catalog#	Reactivity	Applications	Images	Publications
ABCA1 Antibody	NB400-105	Ha, Hu, Mu, Rt	FACS, ICC, IF, IHC-P, IP, WB	(4)	(113)
ABCA1 Antibody (HJ1) - Astrocyte Marker	NB100-2068	Hu, Mu, Rt	IHC-P, WB	(2)	(4)
ABCA1 Antibody (3A1.891.3) - Astrocyte Marker	NB400-164	Hu(-), Mu	WB	(1)	(2)
ABCA1 Antibody (A00121.01) - Astrocyte Marker	NB100-1663	Ch, Hu, Mu	IHC-P, PEP-ELISA, WB	(2)	(3)
ABCA1 Antibody	PAB12504	Ha, Hu, Mu, Rt	FACS, WB
... and 17 more. See All »

RNAi (1)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
ABCA1 RNAi	H00000019-R02	Hu		(1)	(4)

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Images
Rat IgG, H/L Chains Antibody [DyLight 800]	NBP1-72993	Rt	DB, FLISA, IF, WB	(2)
Rat IgG Antibody	NB120-7092	Rt	ELISA, FLISA, IHC-P, IP, WB	(2)
Rat IgG Antibody [Cy3.5]	NB120-6954	Rt	FACS, ICC, IF, IHC-Fr, IHC-P
Rat IgG Antibody [Cy2]	NB120-6952	Rt	FACS, ICC, IF, IHC-Fr, IHC-P
Rat IgG, H/L Chains Antibody [DyLight 549]	NBP1-72812	Rt	FLISA, IF, WB	(2)
See All »

Jump to: Novus Explorer, Research Areas

ABCA1 in Context

Research Areas related to ABCA1 (10):

Novus Explorer for ABCA1 gene

Try our bioinformatic tool that shows the relationships between ABCA1 and other genes, diseases, pathways and post-translational modifications.

ABCA1 Antibody (NB400-165)

ABCA1 Antibody (5A1-1422.11)