ABCA1 Antibody (NB400-164)
ABCA1 Antibody (3A1.891.3) - Astrocyte Marker
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anti-ATP binding Cassette Transporter 1 antibody, anti-ATP-binding cassette sub-family A (ABC1) member 1 antibody, anti-OTTHUMP00000021833 antibody, anti-MGC165011 antibody, anti-MGC164864 antibody, anti-Membrane bound antibody, anti-FLJ14958 antibody, anti-ATP binding cassette sub-family A member 1 antibody, anti-ATP binding cassette sub family A ABC1 member 1 antibody, anti-ATP binding cassette sub family A member 1 antibody, anti-ATP binding Cassette 1 antibody, anti-ABCA 1 antibody, anti-TD antibody, anti-ABC 1 antibody, anti-TGD antibody, anti-HDLDT1 antibody, anti-Cholesterol Efflux Regulatory Protein antibody, anti-CERP antibody, anti-ATP-binding Cassette Transporter 1 antibody, anti-ATP-binding Cassette 1 antibody, anti-ABC-1 antibody, anti-ABC1 antibod, anti-ABC Transporter 1 antibody, |
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ABCA1 Antibody (3A1.891.3) - Astrocyte Marker Summary: | | Gene: | ABCA1 |
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| Purity: | Whole antisera |
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| Host: | Rat |
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| | Specificity: | This is specific for ABCA1. |
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| Publications: | Antibody has been mentioned in at least 2 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional ABCA1 Products ABCA1 Antibody (3A1.891.3) - Astrocyte Marker Details: | Clone: | 3A1.891.3 |
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| Isotype: | IgG1 Kappa |
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| Immunogen: | An A1-N3 peptide (within residues 200-250) of the mouse ABCA1 protein covalently linked to chicken egg albumin by means of glutaraldehyde. |
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| Localization: | Cell Membrane |
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| Marker: | Astrocyte Marker |
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| Epitope: | N-terminal loop |
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Mouse. It does not react with human protein. |
| Uses: | NB 400-164 may be used for Western analysis, where a band is seen at ~250 kDa. This antibody does not appear to work in analysis. **ABCA1 is known to aggregate. Do not boil samples. Use a reducing buffer: 2X sample buffer): 8M Urea, 2% SDS, 715 mM BME, 1% glycerol, 0.001% Bromophenol Blue, 125 mM Tris-HCl [pH 6.8]. |
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| Dilutions: | Western Blot 1:1,000-1:3,000 |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Aliquot and store at -20 °C or -80 °C. Avoid freeze-thaw cycles. |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
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Images (1) Reviews (1) Related Products (25) Exploring ABCA1 Working with ABCA1
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Jump to: Publications, Images, Reviews, Ask a Question
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Working with ABCA1
ABCA1 Antibody (3A1.891.3) - Astrocyte Marker Images (1) Detection of ABCA1 using NB 400-164 at various dilutions in ABCA1 transfected HeLa lysates. |
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Reviewed by:
Anonymous 10/25/2011
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Application:
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Western Blot
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Sample Tested:
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Brain microvessels total proteins
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Sample Loaded:
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20 micrograms
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Species:
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Mouse
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Results:
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We have obtained a specific band of Abca1 at ~ 250 kDa when using this antibody for studying Abca1 expression at the mouse blood-brain barrier. Quantity of protein loading was 20 micrograms.
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Blocking Buffer:
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10 % Milk in TTBS
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Blocking Time:
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1 hour
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Blocking Temperature:
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Room temperature
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Dilution Ratio:
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1:1500
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Incubation Time:
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2 hours
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Incubation Temperature:
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Room temperature
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Incubation Dilution Buffer:
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TTBS
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Description:
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Rabbit anti-rat secondary antibody
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Dilution Ratio:
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1:5000
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Did you observe bands?:
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Yes
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Specific Bands:
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250 kDa
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Publications (2)
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Jump to: Positive Controls, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive ControlsPeptides and ProteinsPrimary Antibodies (22)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rat IgG, H/L Chains Antibody [DyLight 800] | NBP1-72993 | Rt | DB, FLISA, IF, WB | (2) | | | | | Rat IgG Antibody | NB120-7092 | Rt | ELISA, FLISA, IHC-P, IP, WB | (2) | | | | | Rat IgG Antibody [Cy3.5] | NB120-6954 | Rt | FACS, ICC, IF, IHC-Fr, IHC-P | | | | | | Rat IgG Antibody [Cy2] | NB120-6952 | Rt | FACS, ICC, IF, IHC-Fr, IHC-P | | | | | | Rat IgG, H/L Chains Antibody [DyLight 549] | NBP1-72812 | Rt | FLISA, IF, WB | (2) | | | | | See All » |
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