58K Golgi Protein Antibody (NB600-412)
58K Golgi Protein Antibody (58K-9)
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anti-formimidoyltransferase-cyclodeaminase antibody, anti-Formiminotransferase-cyclodeaminase antibody, anti-Golgi Marker antibody, anti-58K Golgi protein antibody, anti-LCHC 1 antibody, anti-Glutamate formiminotransferase antibody, anti-FTCD antibody, anti-Formiminotransferase cyclodeaminase antibody, anti-Formimidoyltransferase cyclodeaminase antibody, |
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58K Golgi Protein Antibody (58K-9) Summary: | | Gene: | FTCD |
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| Purity: | Ascites |
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| Host: | Mouse |
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| | Specificity: | The product recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein. |
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| Publications: | Antibody has been mentioned in at least 7 Publications. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional 58K Golgi Protein Products 58K Golgi Protein Antibody (58K-9) Details: | Clone: | 58K-9 |
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| Isotype: | IgG1 |
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| Immunogen: | Golgi 58K protein purified from rat liver. |
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| Localization: | Cytoplasmic and Golgi Apparatus |
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| Marker: | Golgi Apparatus Marker |
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Human, mouse, rat, canine and bovine. |
| Uses: | This 58K golgi protein antibody (58K-9) is useful for Immunocytochemistry, Immunofluorescence, Immunohistochemistry on formalin-fixed paraffin-embedded sections and Western blot, where a band can be seen at 58 kDa. |
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| Dilutions: | Immunocytochemistry 1:50 - 1:100, Immunofluorescence 1:100 - 1:250, Immunohistochemistry-Paraffin 1:100 - 1:200, Western Blot 1:2000 - 1:5000 |
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| Unit Size: | 0.1 ml (also available: 0.025 ml (sample size)) |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles. |
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| Preservative: | 0.1% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The 58K Golgi Protein is one of the most commonly used Golgi markers. It is known to associate with the PLC gamma 1-PKC epsilon complex which leads to differentiation of certain myoblasts. It also interacts with forminotransferase cyclodeaminase and Sycl1, which is important for Golgi homeostasis during neurodegenerative events. In melanoma, autophagomes generated to feed and enhance melanoma progression display a high concentration of the 58K Golgi protein, probably as a result of ER stress. In colon cancer 58K Golgi Protein co-localizes with collagen XI, and seems to be a promising marker for immunohistopathology studies of colon cancer. Finally, the 58K Golgi protein seems to interact with growth hormone in normal circumstances; however, when growth hormone is misfolded, the interaction is ablated and the Golgi apparatus can become fragmented and incapable of normal ER-Golgi trafficking.
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Images (2) Reviews (0) Related Products (11) Exploring 58K Golgi Protein Working with 58K Golgi Protein
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Jump to: Publications, Images, Protocols/Procedures, Ask a Question
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Working with 58K Golgi Protein
58K Golgi Protein Antibody (58K-9) Images (2) - Golgi Complex Marker-Immunocytochemistry-NB600-412-img0004.jpg "Immunocytochemistry: 58K golgi protein Antibody (58K-9) - Golgi Complex Marker [NB600-412] - 58k golgi protein antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).") Immunocytochemistry: 58K golgi protein Antibody (58K-9) - Golgi Complex Marker [NB600-412] - 58k golgi protein antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red). |  - Golgi Complex Marker-Western Blot-NB600-412-img0003.jpg "Western Blot: 58K Golgi Protein Antibody (58K-9) - Golgi Complex Marker [NB600-412] - WB analysis of 58K golgi protein expression in rat liver tissue using NB600-412.") Western Blot: 58K Golgi Protein Antibody (58K-9) - Golgi Complex Marker [NB600-412] - WB analysis of 58K golgi protein expression in rat liver tissue using NB600-412. |
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Publications (7)58K Golgi Protein Antibody (58K-9) Product Specific:- Azad AK, Torrelles JB, Schlesinger LS. Mutation in the DC-SIGN cytoplasmic triacidic cluster motif markedly attenuates receptor activity for phagocytosis and endocytosis of mannose-containing ligands by human myeloid cells. J Leukoc Biol. 2008 Dec;84(6):1594-603. PubMed PMID: 18772280.
- Young KG, Pinheiro B, Kothary R. A Bpag1 isoform involved in cytoskeletal organization surrounding the nucleus. Exp Cell Res. 2006 Jan 15;312(2):121-34. PubMed PMID: 16289082.
more... - Hennig D, Scales SJ, Moreau A, Murley LL, De Mey J, Kreis TE. A formiminotransferase cyclodeaminase isoform is localized to the Golgi complex and can mediate interaction of trans-Golgi network-derived vesicles with microtubules. J Biol Chem. 1998 Jul 31;273(31):19602-11. [IF]
- Lippincott-Schwartz J, Cole NB, Marotta A, Conrad PA, Bloom GS. Kinesin is the motor for microtubule-mediated Golgi-to-ER membrane traffic. J Cell Biol. 1995 Feb;128(3):293-306.
- Gao YS, Alvarez C, Nelson DS, Sztul E. Molecular cloning, characterization, and dynamics of rat formiminotransferase cyclodeaminase, a Golgi-associated 58-kDa protein. J Biol Chem. 1998 Dec 11;273(50):33825-34. [WB, IF]
- Bloom GS, Brashear TA. A novel 58-kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem. 1989 Sep 25;264(27):16083-92. [WB, IF, rat]
- Bashour AM, Bloom GS. 58K, a microtubule-binding Golgi protein, is a formiminotransferase cyclodeaminase. J Biol Chem. 1998 Jul 31;273(31):19612-7. [WB, rat]
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| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
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