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53BP1 Antibody (NB100-904) (0.1 ml)

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	Catalog Number	NB100-904 (Sample Size Available)
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(see alternate names)

anti-tumor suppressor p53-binding protein 1 antibody, anti-p53-binding protein 1 antibody, anti-tumor protein 53-binding protein, 1 antibody, anti-tumor protein p53-binding protein, 1 antibody, anti-MGC138366 antibody, anti-p202 antibody, anti-p53BP1,53BP1FLJ41424 antibody, anti-tumor protein p53 binding protein 1 antibody

53BP1 Antibody Summary:

Species:	Hu, Mu	Gene:	TP53BP1
Applications:	WB, ICC, IHC-P	Purity:	Affinity purified
Clonality:	Polyclonal	Host:	Rabbit
Specificity:	NB100-904 is specific for human 53BP1 protein.
Publications:	NB100-904 has been mentioned in at least 24 Publications

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53BP1 Antibody Details:

Immunogen:	A synthetic peptide, which represented a portion of human tumor protein p53-binding protein 1 encoded in exon 11, 12 and 19 (LocusLink ID 7158). This antibody is a cocktail hybrid of NB 100-304 and NB 100-305.

Species Reactivity:

Reacts with human in Western Blot, Immunohistochemistry, and immunocytochemistry. Reacts with mouse in immunocytochemistry (Western Blot has not been done using this antibody on mouse protein).

Applications:

Uses:	This 53BP1 antibody is useful for Western Blot where a band at ~220 kD is seen on SDS-PAGE, Immunocytochemistry, and IHC-paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Irradiated lysates demonstrate a gel shift. This antibody has not yet been tested in immunoprecipitation.
Dilutions:	Immunocytochemistry 1:100 - 1:1000, Immunohistochemistry-Paraffin 1:400, Western Blot 1:1000 - 1:10000
Unit Size:	0.1 ml (also available: 0.025 ml (sample size))
Concentration:	1.0 mg/ml
Positive Controls:	1 Positive Controls
Conjugated Antibodies:	5 Conjugated Antibodies

Packaging:

Storage:	Store at 4°C. Do not freeze.
Buffer:	Tris-citrate/phosphate (pH 7-8) buffer.
Preservative:	0.1% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: TP53BP1

Entrez:	7158 (Human)
Uniprot:	Q12888 (Human)

Related Genes:

Background:

P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.

Images (3)



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53BP1 Antibody Images (3)

	Western Blot: 53BP1 Antibody [NB100-904] - Figure 1 illustrates Western blot detection of 53BP1 (220 kDa) from untreated U2OS cell lysate using NB100-904.

	Immunohistochemistry: 53BP1 Antibody [NB100-904] - IHC analysis of 53BP1 in human renal cancer using DAB with hematoxylin counterstain.

	Immunocytochemistry: 53BP1 Antibody [NB100-904] - 53BP1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 594 (red).

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Publications (24)

53BP1 Antibody Product Specific:

Hewitt, G. et al. Telomeres are favoured targets of a persistent DNA damage response in ageing and stress-induced senescence. Nat. Commun (2012). [PMID: 22426229]
Velummailum R. Involvement of NLRP5 in the maintenance of genome integrity in murine oocytes [Internet]. 2011

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Protocols

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Jump to: Conjugated Antibodies, Positive Controls, Antibody Packs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies

Related Products

Conjugated Antibodies

Name	Catalog#	Reactivity	Applications
53BP1 Antibody [DyLight 550]	NB100-904R	Hu, Mu	ICC, WB
53BP1 Antibody [HRP]	NB100-904H	Hu, Mu	ICC, WB
53BP1 Antibody [DyLight 488]	NB100-904G	Hu, Mu	ICC, WB
53BP1 Antibody [DyLight 650]	NB100-904C	Hu, Mu	ICC, WB
53BP1 Antibody [Biotin]	NB100-904B	Hu, Mu	ICC, WB

Positive Controls

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
Other Positive Controls U205 cell lysates

Antibody Packs (1)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
53BP1 Antibody Pack 2 vials	NB100-926	Hu, Mu	ICC, IF, WB	(6)	(1)

Lysates (1)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
53BP1 Lysate	NBP2-10450

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
53BP1 Partial Recombinant Protein	H00007158-Q01	Hu	ELISA, WB	(1)
53BP1 Peptide	NB100-305PEP

Primary Antibodies (22)

Name	Catalog#	Reactivity	Applications	Images	Publications
53BP1 Antibody	NB100-304	Hu, Mu	FACS, ICC, WB	(6)	(23)
53BP1 Antibody	NB100-305	Hu, Mu	ICC, IF, WB	(3)	(13)
53BP1 [p Ser25] Antibody	NB100-1803	Hu, Mu	FACS, IP, PLA, WB	(2)	(4)
53BP1 Antibody	NBP1-19447	Hu, Mu, Rt	IF, IHC-P, WB	(1)
53BP1 Antibody (1B9)	H00007158-M01	Hu	ELISA	(1)
... and 17 more. See All »

RNAi (1)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
53BP1 RNAi	H00007158-R01	Hu		(1)	(4)

Secondary Antibodies

Name	Catalog#	Reactivity	Applications	Images	Publications
Rabbit IgG Antibody [Cy3]	NB120-6939	Rb	FACS, FLISA, ICC, IF, IHC-Fr, IHC-P, MiAr		(2)
Rabbit IgG Antibody [HRP]	NB730-H	Rb	ELISA, ICC, IHC-P, WB		(6)
Rabbit IgG Antibody [FITC]	NB730-F	Rb	ICC, IHC-P		(1)
Rabbit IgG Antibody [Biotin]	NB730-B	Rb	ELISA, ICC, IHC-P, WB
Rabbit IgG, H/L Chains Antibody [DyLight 488]	NBP1-72944	Rb	FLISA, IF, WB	(3)
See All »

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53BP1 in Context

Research Areas related to 53BP1 (6):