Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
Human and mouse. Other species have not been tested.
Applications:
Uses:
The antibodies included in this pack are useful for Western blot and immunocytochemistry. See individual datasheets for suggested working dilutions.
Dilutions:
Immunocytochemistry, Immunofluorescence, Western Blot
Unit Size:
2 vials
Concentration:
Concentration of individual antibodies may be found on the vial label.
Notes:
1 vial each of: NB100-304 and NB100-305.
Packaging:
Storage:
Store at 4 °C. Do not freeze.
Buffer:
Tris-citrate/phosphate buffer, pH 7 to 8
Preservative:
0.09% Sodium Azide
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
We know how important it is to your research to have access to antibodies specific for your model. As it is often impossible to know which antibody will work best for a specific sample and application, we now offer SuperNovus Packs. These packs include different antibodies to the same protein to give you the ability to decide for yourself. The SuperNovus Packs are all offered at a discount price compared to purchasing each antibody individually. P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. This also suggests a role in DNA repair, maintaining genomic stability.
Immunofluorescence: 53BP1 antibody [NB100-304] - 53BP1 foci in proliferating MO59K cells; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours. Images captured in a Kodak digital image system on a Leica fluorescent microscope.
Immunofluorescence: 53BP1 antibody [NB100-304] - 53BP1 foci in proliferating MEFs; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours. Images captured in a Kodak digital image system on a Leica fluorescent microscope.
Flow Cytometry: 53BP1 Antibody [NB100-304] - Flow cytometric detection of 53bp1. 1 million Jurkat cells were fixed, permeabilized, and stained with 1.5 mcg/ml anti-53bp1 NB100-304 in a 150 mcl reaction. Isotype control (black), anti-53bp1 (red).
Western Blot: 53BP1 Antibody [NB100-304] - Detection of Human [deleted mouse] 53BP1 by Western Blot on Whole cell lysate from U2OS or 293T cells resolved on a 3 to 8% tris-acetate gel using NB100-304.
Western Blot: 53BP1 Antibody [NB100-305] - Western Blot analysis of human 53BP1, using NB100-305. Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel.