293 Whole Cell Lysate (NB800-PC6)
293 Whole Cell Lysate
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293 Whole Cell Lysate Summary: | | | Specificity: | 293 whole cell extract lysate |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional 293 Whole Cell Products Lysate Details: | Type: | Cell | Life Stage: | Adult | | Tissue: | Digestive/Waste | Tissue Condition: | Normal | | Sub-cellular Fraction: | Whole |
| Uses: | Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Heat lysate to 95 degrees C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating.
The recommended loading volume per lane is 0.01-0.02 ml depending on the size format of your gel. |
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| Dilutions: | Western Blot |
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| Unit Size: | 0.5 mg |
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| Concentration: | 1 mg/ml |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at -80 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8] |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
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Images (1) Reviews (0) Related Products (3)
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Working with 293 Whole Cell
293 Whole Cell Lysate Images (1) Western Blot: 293 Whole Cell Lysate [NB800-PC6] - Lane 1: Coommassie stain of 0.02 ml 293 extract (NB 800-PC6), separated in a 4-20% gradient gel under reducing conditions. Lane 2: Molecular weight standard. |
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Jump to: Positive Controls, Lysates
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Related ProductsPositive Controls| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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Other Positive Controls- 293 lysates are only applicable as a positive control for specific antibodies.
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Lysates (3)
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