293 Whole Cell Lysate Summary:
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Species:
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Hu
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Gene:
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Applications:
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WB, PAGE
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Purity:
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Whole antisera
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Clonality:
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Host:
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Specificity:
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293 whole cell extract lysate
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Publications:
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View Additional 293 Products
Lysate Details:
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Type:
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Cell
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Sub-cellular Fraction:
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Whole
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Uses:
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Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents . Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.
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Dilutions:
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SDS-Page
Western Blot
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Unit Size:
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0.5 mg
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Concentration:
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mg/ml
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Positive Controls:
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1 Positive Controls
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Packaging:
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Storage:
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Store at -80°C. Avoid freeze-thaw cycles.
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Buffer:
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1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8]
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Limitations:
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This product is for research use only and is not approved
for use in humans or in clinical diagnosis. Products are
guaranteed for 6 months from date of receipt, except for
peptides and proteins which are guaranteed for 3 months.
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Related Genes
Related Genes:
Background:
This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.
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Images (2)
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![Western Blot: 293 Whole Cell Lysate [NB800-PC6] - Lane 1: Coommassie stain of 0.02 ml 293 extract (NB 800-PC6), separated in a 4-20% gradient gel under reducing conditions. Lane 2: Molecular weight standard.](http://images.novusbio.com/images/293 Whole Cell Lysate-Western Blot-NB800-PC6-img0001.jpg "Western Blot: 293 Whole Cell Lysate [NB800-PC6] - Lane 1: Coommassie stain of 0.02 ml 293 extract (NB 800-PC6), separated in a 4-20% gradient gel under reducing conditions. Lane 2: Molecular weight standard.")
![SDS-Page: 293 Whole Cell Lysate [NB800-PC6] - Coommassie stained SDS-PAGE of 20 ug of A 549 WCL, Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.](http://images.novusbio.com/images2/293 Whole Cell Lysate-SDS-Page-NB800-PC6-img0002.jpg "SDS-Page: 293 Whole Cell Lysate [NB800-PC6] - Coommassie stained SDS-PAGE of 20 ug of A 549 WCL, Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.")
