293 Whole Cell Lysate 0.5 mg

293 NB800-PC6

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Ordering Information: NB800-PC6

Catalog NumberNB800-PC6
  • Size(s)0.5 mg
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293 Whole Cell Lysate Summary

 
Species Human
Tested Applications WB, PAGE
Purity Whole antisera
Specificity 293 whole cell extract lysate
Guarantee Plus All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.

Lysate Details for 293

 
Type Cell
Subcellular Fraction Whole

Applications/Dilutions

 
Dilutions
  • Western Blot
  • SDS-Page
Application Notes

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents . Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Positive Controls

1 Positive Control

Product PDFs

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Controls & Support Products

Packaging, Storage & Formulations

 
Storage Store at -80C. Avoid freeze-thaw cycles.
Buffer 1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8]
Unit Size 0.5 mg
Concentration Please contact technical services for concentration.
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Background

 
This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.