293 Whole Cell Lysate 0.5 mg

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Western Blot: 293 Whole Cell Lysate [NB800-PC6] - Lane 1: Coommassie stain of 0.02 ml 293 extract (NB 800-PC6), separated in a 4-20% gradient gel under reducing conditions. Lane 2: Molecular weight standard.
SDS-Page: 293 Whole Cell Lysate [NB800-PC6] - Coommassie stained SDS-PAGE of 20 ug of A 549 WCL, Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.
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Ordering Information: NB800-PC6

  • Catalog Number
    NB800-PC6
  • Size(s)
    0.5 mg
  • Availability
  • Price
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293 Whole Cell Lysate Summary

Species Human
Tested Applications WB, PAGE
Purity
Whole antisera
Specificity
293 whole cell extract lysate
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Lysate Details for 293

Type
Cell
Subcellular Fraction
Whole

Applications/Dilutions

Dilutions
  • Western Blot
  • SDS-Page
Application Notes
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents . Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.
Positive Controls

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Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8]
Unit Size
0.5 mg
Concentration
Please contact technical services for concentration.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Background

This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

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Publications for 293 Lysate (NB800-PC6) (0)

There are no publications for 293 Lysate (NB800-PC6).
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Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Product General Protocols

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Video Protocols

WB Video Protocol

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FAQs for 293 (0)

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Applications WB

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Species Human
Applications WB, IP, PAGE

     1 Review

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Applications WB

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Applications WB, PAGE

NB800-PC11
Western Blot: Raji Whole Cell Lysate [NB800-PC11] - Coommassie stain of 0.02 ml of NB 800-PC11 separated on a 4-20% gradient gel under reducing conditions (lane 2). MW standard is shown in lane 1.SDS-Page: Raji Whole Cell Lysate [NB800-PC11] - Coommassie stained SDS-PAGE of 20 ug of A 549 WCL, Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.


Applications WB, PAGE

NBP1-30157
Western Blot: Raw 264.7 Whole Cell Lysate [NBP1-30157] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2) [NBP1-30157].


Applications WB

NB800-PC9
Western Blot: HeLa Nuclear Cell Lysate [NB800-PC9] - Analysis of Histone H3 (NBP1-30141) uisng HeLa nuclear lysate [NB800-PC9].SDS-Page: HeLa Nuclear Cell Lysate [NB800-PC9] - Staining of HeLa nuclear lysate using NB800-PC9.


Applications WB, PAGE

NBP1-78158


Applications WB

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