Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The lysate was created in HEK293T cells, using plasmid ID RC200903 and based on accession number NM_002813. The protein contains a C-terminal DDK tag.
Lysate Details:
Type:
Overexpression
Life Stage:
Adult
Protein State:
Native
Tissue Condition:
Normal
Sub-cellular Fraction:
Whole
Applications:
Uses:
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Dilutions:
Western Blot
TMW:
24.5 kDa
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Notes:
This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes. Please note that “1 mg/ml” refers to the total protein concentration and that the specific protein concentration is not determined at this time.
Packaging:
Storage:
Store at -80 °C. Avoid freeze-thaw cycles.
Buffer:
RIPA buffer
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.