p27/Kip1 Antibody

Name: p27/Kip1 Antibody
Brand: Novus Biologicals
SKU: NB600-1442
Price: 449 USD
Availability: Discontinued

(1 Publication)

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Summary

Product Discontinued

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Catalog Number

NB600-1442
Availability

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p27/Kip1 Antibody Summary

Description	This antibody was prepared from monospecific antiserum by delipidation and defibrination.
Immunogen	This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length recombinant human p27/Kip1 protein.
Localization	Nuclear
Specificity	No reaction is observed against other related tumor supressor proteins.
Isotype	Serum
Clonality	Polyclonal
Host	Rabbit
Gene	CDKN1B
Purity	Delipidation and Defibrination
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

ELISA 1:10000-1:40000
Immunoprecipitation 1:100
Simple Western
Western Blot 1:1000-1:4000

Application Notes

This antibody is suitable for use in ELISA, immunoprecipitation, co-immunoprecipitation and western blotting. Expect a band at ~27 kDa in size corresponding - p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. No reactivity is observed with p27 from mouse. This antibody is suitable for immmunoprecipitation of in vitro translated protein and cell lysates and will co-precipitate associated proteins.

Control

26S proteasome subunit 9 Overexpression Lysate

Publications

Read Publication using NB600-1442.

Reactivity Notes

Antiserum will specifically react with p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse. Reactivity against p27 from other species hasn't been tested.

Packaging, Storage & Formulations

Storage	Store at -20C. Avoid freeze-thaw cycles.
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	0.01% Sodium Azide
Concentration	80 mg/ml
Purity	Delipidation and Defibrination

Alternate Names for p27/Kip1 Antibody

CDKN1B
CDKN4
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
cyclin-dependent kinase inhibitor 1B
Cyclin-dependent kinase inhibitor p27
Kip1
KIP1P27KIP1
MEN1B
MEN4
p27
p27Kip1

Background

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p27/Kip1 Antibody (NB600-1442)(1)

Showing Publication 1 - 1 of 1.

Publication using NB600-1442	Applications	Species
Kushwaha SS, Raichlin E, Sheinin Y et al. Sirolimus affects cardiomyocytes to reduce left ventricular mass in heart transplant recipients. Eur Heart J;29(22):2742-2750. 2008-01-01 [PMID: 18790727]

Reviews for p27/Kip1 Antibody (NB600-1442) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for p27/Kip1 Antibody (NB600-1442) (0)

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Control Lysate(s)

26S proteasome subunit 9 Overexpression Lysate

Secondary Antibodies

Isotype Controls

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Bioinformatics

Gene Symbol	CDKN1B
Entrez	5715(Human) 67151(Mouse) 161475(Rat)

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Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

p27/Kip1 Antibody

Species Glossary

Images

Product Details

Order Details

p27/Kip1 Antibody Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for p27/Kip1 Antibody

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for p27/Kip1 Antibody (NB600-1442)(1)

Reviews for p27/Kip1 Antibody (NB600-1442) (0)

Product General Protocols

Video Protocols

FAQs for p27/Kip1 Antibody (NB600-1442) (0)

Control Lysate(s)

Secondary Antibodies

Isotype Controls

Additional p27/Kip1 Products

Research Areas for p27/Kip1 Antibody (NB600-1442)

Blogs on p27/Kip1

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics