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![Western Blot: 26S Proteasome Antibody [NB600-1442] - Western blot using affinity purified anti-p27 antibody shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight 800 conjugated Gt-a-Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lanes M). Other detection systems will yield similar results.](http://images.novusbio.com/images/26S Proteasome Antibody-Western Blot-NB600-1442-img0001.jpg "Western Blot: 26S Proteasome Antibody [NB600-1442] - Western blot using affinity purified anti-p27 antibody shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight 800 conjugated Gt-a-Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lanes M). Other detection systems will yield similar results.")
