160kDa Neurofilament Medium Antibody (NBP1-40724)

160kDa Neurofilament Medium Antibody (EP2460)

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160kDa Neurofilament Medium Antibody (EP2460) Summary:
Species:Hu
Applications:ICC, IHC-P, IP, WB
Clonality:Monoclonal
Gene:NEFM
Purity:IgG purified
Host:Rabbit
 
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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160kDa Neurofilament Medium Antibody (EP2460) Details:
Clone:EP2460
Isotype:IgG
Immunogen:A synthetic peptide corresponding to residues in human Neurofilament M was used as an immunogen.
Marker:Neuronal Marker
Species Reactivity:

Reacts in Human.

Applications:
Uses:This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 160kDa.
Dilutions:Western Blot 1:100000-500000, Immunohistochemistry-Paraffin 1:250-500, Immunocytochemistry 1:100-250, Immunoprecipitation 1:10
TMW:160 kDa
Unit Size: 0.1 ml
Concentration:Please see the vial label for concentration.
Packaging:
Storage:Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:50mM Tris-Glycine [pH 7.4] containing 0.15M NaCl, 40% Glycerol and 0.05% BSA
Preservative:0.01% Sodium Azide
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: NEFM
Swiss ProtP07197 (Human)
 
Background:

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.

 
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160kDa Neurofilament Medium Antibody (EP2460) Images (4)
Immunohistochemistry: 160kDa Neurofilament Medium Antibody (EP2460) [NBP1-40724] - Immunohistochemical analysis of paraffin-embedded human normal brain tissue using anti-Neurofilament MImmunohistochemistry: 160kDa Neurofilament Medium Antibody (EP2460) [NBP1-40724] - Immunohistochemical analysis of paraffin-embedded human glioma using anti-Neurofilament M
Western Blot: 160kDa Neurofilament Medium Antibody (EP2460) [NBP1-40724] - Western blot analysis on (A) 293T and (B) SH-SY5Y cell lysates using anti-Neurofilament MImmunofluorescence: 160kDa Neurofilament Medium Antibody (EP2460) [NBP1-40724] - Immunofluorescent staining of SH-SY-5Y cells using anti-Neurofilament M
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Jump to: Lysates, Primary Antibodies, Secondary Antibodies

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Lysates (1)
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160kDa Neurofilament Medium LysateNBL1-13578WB(1)

Primary Antibodies (13)
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160kDa Neurofilament Medium AntibodyNB300-133Av, Bv, Fe, Hu, Ma, Mu, Po, RtICC, IF, IHC-Fr, IHC-P, WB(1)(3)

160kDa Neurofilament Medium Antibody (3H11)NB300-134Av, Hu, Ma, MuIF, IHC-P, WB(2)(2)

160kDa Neurofilament Medium AntibodyNB300-222Ch, Fe, Fi, Hu, Mu, RtIF, WB(2)

160kDa Neurofilament Medium Antibody (NF-09)NB500-446Bv, Hu, Ma, Mu, Po, RtICC, IHC-P, WB(1)(1)

160kDa Neurofilament Medium AntibodyNBP1-87754HuIF, IHC-P, WB(1)

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Secondary Antibodies
NameCatalog#ReactivityApplicationsImagesPublicationsReviews
Rabbit IgG Antibody [HRP]NB730-HRbELISA, ICC, IHC-P, WB(5)

Rabbit IgG Antibody [FITC]NB730-FRbICC, IHC-P(1)

Rabbit IgG Antibody [Biotin]NB730-BRbELISA, ICC, IHC-P, WB

Rabbit IgG, H/L Chains Antibody [DyLight 488]NBP1-72944RbFLISA, IF, WB(3)

Rabbit IgG Antibody [Alkaline Phosphatase]NB730-APRbELISA, ICC, IHC-P, WB(1)

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