14-3-3 zeta Antibody Summary
| Immunogen |
Synthetic peptide, corresponding to amino acids 49-100 of Human 14-3-3 zeta. |
| Specificity |
14-3-3 zeta (A79) detects endogenous levels of 14-3-3 zeta protein. |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
YWHAZ |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin 1:50-1:200
- Western Blot 1:500-1:1000
|
| Application Notes |
Western blot (WB) analysis of 14-3-3Zeta(A79) pAb pAb in extracts from HeLa cells. Immunohistochemistry (IHC) analyzes of 14-3-3Zeta(A79) pAb in paraffin-embedded human brain tissue. |
| Theoretical MW |
28 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS (pH 7.2) |
| Preservative |
0.05% Sodium Azide |
| Concentration |
1.0 mg/ml |
| Purity |
Immunogen affinity purified |
Alternate Names for 14-3-3 zeta Antibody
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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