14-3-3 zeta [p Ser58] Antibody Pair Summary:

Species:	Hu
Applications:	PLA

Gene:	YWHAZ

Specificity:	Reacts with tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide.

Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.

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Peptides and Proteins	Primary Antibodies

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14-3-3 zeta [p Ser58] Antibody Pair Details:

Modification:	p Ser58

Species Reactivity:

Human

Applications:

Uses:	This product is useful for In Situ Proximity Ligation Assay (cell).
Dilutions:	Proximity Ligation Assay
Unit Size:	1 set
Concentration:	Concentration of individual antibodies may be found on the vial label.

Notes:

Antibody pair set content: 1. Phospho-YWHAZ S58 rabbit MaxPab affinity purified polyclonal antibody (20 ul) In PBS, 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol) 2. YWHAZ mouse purified polyclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.

Packaging:

Storage:	Store at -80 °C. Avoid freeze-thaw cycles.
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: YWHAZ

Entrez	7534 (Human)

Background:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]

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Working with 14-3-3 zeta

14-3-3 zeta [p Ser58] Antibody Pair Images (1)

Immunofluorescence: 14-3-3 zeta [phospho Ser58] Antibody Pair [DP0272] - Representative image of Proximity Ligation Assay (PLA) of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit affinity purified polyclonal antibody 1:1200 and mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents one single phosphorylated protein.

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Jump to: Lysates, Peptides and Proteins, Primary Antibodies

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Lysates (2)

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
14-3-3 zeta Lysate	H00007534-T01	Hu	WB	(2)
14-3-3 zeta Lysate	NBL1-17952		WB	(1)

Peptides and Proteins

Name	Catalog#	Reactivity	Applications	Images	Publications	Reviews
14-3-3 zeta Partial Recombinant Protein	H00007534-Q01		ELISA, WB

Primary Antibodies (11)

Name	Catalog#	Reactivity	Applications	Images	Publications
14-3-3 zeta Antibody	NBP1-61188	Hu, Mu, Rt	ELISA, IF, IHC, WB	(3)
14-3-3 zeta [p Ser58] Antibody	NB100-81766	Hu, Mu, Rt	IF, IHC-P, WB	(3)	(3)
14-3-3 zeta Antibody	NB100-91646	Hu, Mu, Rt	ELISA, IF, IHC-P, IP, WB	(2)
14-3-3 zeta Antibody	NBP1-72171	Hu, Mu, Rt	ELISA, IHC-P, IP, WB	(2)	(1)
14-3-3 zeta Antibody	NBP1-19623	Hu, Mu, Rt	IHC-P, WB	(2)
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14-3-3 zeta in Context

Research Areas related to 14-3-3 zeta (1):

Cell Cycle and Replication

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14-3-3 zeta Antibody Pair (DP0272)

14-3-3 zeta [p Ser58] Antibody Pair