14-3-3 zeta [p Ser58] Antibody Pair (DP0272)

14-3-3 zeta [p Ser58] Antibody Pair (DP0272) ( 1 set)

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	Catalog Number	DP0272
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14-3-3 zeta [p Ser58] Antibody Pair Summary:

Species:	Hu	Gene:	YWHAZ
Applications:	PLA	Purity:
Clonality:		Host:
Specificity:	Reacts with tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide.
Publications:

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14-3-3 zeta [p Ser58] Antibody Pair Details:

Modification:	p Ser58

Species Reactivity:

Human

Applications:

Uses:	This product is useful for In Situ Proximity Ligation Assay (cell).
Dilutions:	Proximity Ligation Assay
Unit Size:	1 set
Concentration:	Concentration of individual antibodies may be found on the vial label.

Notes:

Antibody pair set content: 1. Phospho-YWHAZ S58 rabbit MaxPab affinity purified polyclonal antibody (20 ul) In PBS, 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol) 2. YWHAZ mouse purified polyclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.

Packaging:

Storage:	Store at -80°C. Avoid freeze-thaw cycles.
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: YWHAZ

Entrez:	7534 (Human)

Related Genes:

Background:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]

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14-3-3 zeta [p Ser58] Antibody Pair Images (1)

Immunofluorescence: 14-3-3 zeta [phospho Ser58] Antibody Pair [DP0272] - Representative image of Proximity Ligation Assay (PLA) of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit affinity purified polyclonal antibody 1:1200 and mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents one single phosphorylated protein.