Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
The lysate was created in HEK293T cells, using plasmid ID RC201925 and based on accession number NM_012479. The protein contains a C-terminal DDK tag.
Lysate Details:
Type:
Overexpression
Life Stage:
Adult
Protein State:
Native
Tissue Condition:
Normal
Sub-cellular Fraction:
Whole
Applications:
Uses:
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Dilutions:
Western Blot
TMW:
28.1 kDa
Unit Size:
0.1 mg
Concentration:
1.0 mg/ml
Notes:
This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes. Please note that “1 mg/ml” refers to the total protein concentration and that the specific protein concentration is not determined at this time.
Packaging:
Storage:
Store at -80 °C. Avoid freeze-thaw cycles.
Buffer:
RIPA buffer
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.